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OriGene联合CELL杂志共同出版的
CRISPR论文集
Video overview of cDNA clone
offering
Video brochure for cDNA clone
OriGene cDNA clones in recent publications
Epilepsy-associated GRIN2A mutations reduce NMDA receptor trafficking and agonist potency - molecular profiling and functional rescue Sci Rep 2017 [GRIN2A]

Matrix metalloproteinase processing of PTHrP yields a selective regulator of osteogenesis, PTHrP1-17 Oncogene 2017 [PTH1R]

Whole-Exome Sequencing of Congenital Glaucoma Patients Reveals Hypermorphic Variants in GPATCH3, a New Gene Involved in Ocular and Craniofacial Development. Sci Rep 2017 [GPATCH3]

Ziploc-ing the Structure 2.0: endoplasmic reticulum resident peptidyl prolyl isomerases show different activities toward hydroxyproline J. Biol. Chem. 2017 [FKBP2]

Home CRISPR/Cas9

CRISPR/Cas9 Genome Editing

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Click to view the details of CRISPR/CAS9
Genome-editing
Knockout Kit
CRISPR/Cas
Vectors
gRNA Cloning and
Donor Construction
CRISPR/Cas
Starter Kit

Features

Knockout Kit Finder
Search by gene symbol or keyword.
  • 靶向基因ORF区域5'段的基因敲除试剂盒
  • 针对同一基因,提供2条向导RNA的 pCas-Guide 载体以便保证有效的切割
  • 预设计好的带有同源臂的供体载体
  • 敲入GFP和Puromycin作为筛选标记
  • pCas-Guide-scramble 作为阴性对照
CAS9 Antibody
TA190309 New!

应用

  • 敲入GFP报告基因,可以用于启动子研究
  • 染色体水平的基因敲除

Scheme of Genome-editing knockout kit using CRISPR

Diagram-Gene Editing RecombII

Overview

OriGene公司最新推出基因组编辑工具“CRISPR/Cas9系统”,该系统灵活简单、可以对特定基因组位点进行切割置换的,特异性高、细胞毒性低。CRISPR/Cas9系统是利用Cas9核酸酶在向导RNA的指引下通过识别基因组的任何位点保守的间隔相邻基序(PAM,序列为NGG),在PAM的上游对DNA进行定点切割。使用pCas-Guide系统可以很简便的为内源性基因标记标签。除此之外,pCas-Guide系统的供体载体(rescue donor vectors)也可以用于其他的基因组工程,如基因抑制(突变,缺失,插入),用于启动子研究的荧光素酶报告基因置换或绿色荧光蛋白基因置换,基因敲入以及用于治疗的基因修复等。

Learn from a 4-min Youtube Video

Frequently Asked Questions

Validation data for pCas-Guide system PDF

References

 

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