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Home CRISPR/Cas9 Cas9 Insertion at AAVS1

Cas9 Insertion at AAVS1

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Cas9 stable human cell lines for CRISPR mediated genome editing, the best way is to insert Cas9 at a safe harbor location, AAVS1 locus. CRISPR technology can be used to achieve this. Two components are needed: All-in-one AAVS1 gRNA targeting vector and Cas9 AAVS1 donor vector. AAVS1 targeting vector cuts AAVS1 locus, Cas9 and a selection cassette from the donor vector containing the AAVS1 homologous arms will integrate into the AAVS1 locus.


AAVS1 gRNA Vector

Cat # Product Name Description Price Availability Manual
GE100023 pCas-Guide-AAVS1 AAVS1 gRNA vector, validated AAVS1 targeting sequence cloned in pCas-Guide vector ¥3420 2-3周 Manual

Cas9 AAVS1 Donor Vectors

Cat # Product Name Description Price Availability Manual
GE100037 pAAVS1-Cas9-Puro-DNR Cas9 expression vector in AAVS1 donor vector, puromycin selection (10 ug) ¥3420 2-3周 Manual
GE100038 Cas9 AAVS1 insertion kit (Puro) Vector kit to insert Cas9 at AAVS1 locus (puro selection), including AAVS1 gRNA vector (cat# GE100023), Cas9-Puro donor (cat# GE100037) and a scramble gRNA control (cat# GE100003) ¥6365 2-3周 Manual
GE100039 pAAVS1-Cas9-BSD-DNR Cas9 expression vector in AAVS1 donor vector, blasticidin selection (10ug) ¥3420 2-3周 Manual
GE100040 Cas9 AAVS1 insertion kit (BSD) Vector kit to insert Cas9 at AAVS1 locus (blasticidin selection), including AAVS1 gRNA vector (cat# GE100023), Cas9-BSD donor (cat# GE100039), and a scramble gRNA control (cat# GE100003) ¥6365 2-3周 Manual

Cas9 AAVS1 Insertion Diagram

AAVS1_simple_diagram

Related Products

AAVS1 Transgene Insertion CRISPR Vectors Gene Knockout Kits


 

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