pAAVS1-Puro-DNR

CAT＃: GE100024

Donor vector with AAVS1 homologous arms, C-terminal Myc-DDK tag, Puro resistance, ready for transgene cloning

Custom Cloning into Donor Vector Request

Datasheet
SDS
Vector Sequence

CNY 7,510.00

货期*
现货
规格
    • 10 ug
Cited in 7 publications.

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Purified recombinant protein of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E.coli, sterile-filtered, suitable for cell culture
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Purified recombinant protein of mutant(D10A) of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E. coli
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Specifications

Product Data
Function AAVS1 donor vector
Features
  • Donor vector for targeted transgene insertion into human AAVS1 site
  • Contains left and right AAVS1 homologous sequences (550 bp each side)
  • Multiple cloning sites for transgene cloning, compatible for shuttling from the TrueORF clones
  • Puromycin mammalian selection under PGK promoter
  • Targeted insertion when cotransfected with pCas-Guide-AAVS1 (SKU GE100023)
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (7)

The use of this Vectors has been cited in the following citations:
GigaAssay – An adaptable high-throughput saturation mutagenesis assay platform ,null, Genomics ,PubMed ID 35905834
Safe harbor-targeted CRISPR-Cas9 homology-independent targeted integration for multimodality reporter gene-based cell tracking ,null, Science Advances ,PubMed ID 33523917
Repurposing of KLF5 activates a cell cycle signature during the progression from a precursor state to Oesophageal Adenocarcinoma ,Rogerson, C;Ogden, S;Britton, E;Ang, Y;Sharrocks, A;, ;, bioRxiv ,PubMed ID 32880368 [pAAVS1-Puro-DNR]
A Safe Harbor-Targeted CRISPR/Cas9 Homology Independent Targeted Integration (HITI) System for Multi-Modality Reporter Gene-Based Cell Tracking ,Kelly, JJ;Saee-Marand, M;Nystrom, NN;Chen, Y;, bioRxiv [pAAVS1-Puro-DNR]
Repurposing of KLF5 activates a cell cycle signature during the progression from Barrett\'s oesophagus to oesophageal adenocarcinoma. ,Rogerson, C;Ogden, S;Britton, E;Ang, Y;Sharrocks, AD;, bioRxiv [pAAVS1-Puro-DNR]
Non-Invasive Fluorescent Monitoring of Ovarian Cancer in an Immunocompetent Mouse Model ,Wilson, AL;Wilson, KL;Bilandzic, M;Moffitt, LR;Makanji, M;Gorrell, MD;Oehler, MK;Rainczuk, A;Stephens, AN;Plebanski, M;, Cancers (Basel) 2018 ,PubMed ID 30602661 [pAAVS1-Puro-DNR]
Safe Harbor Targeted CRISPR-Cas9 Tools for Molecular-Genetic Imaging of Cells in Living Subjects. ,null, The CRISPR journal ,PubMed ID 31021241

Documents

Product Manuals
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SDS
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