OriGene Technologies, Inc.
  邮箱:  密码: 忘记密码?
注册 | 购物车 | 主页
搜索:    
About FL cDNA Clones RNAi Gene Expression Lysates and Proteins Antibodies Tissue IVD 原料 Other Products Tech Support
orange
按通路检索:
按应用检索:
按蛋白家族检索:
按产品线检索:
按抗体类型检索:
按应用种属检索:
按宿主来源检索:
TrueMABTM Antibodies - Made against Authentic Protein Antigens

Anti-CHEK2 TRUEMAB Antibody Clone OTI5C4

div

Specifications Related Products Product Reviews FAQs
Catalog No. Description Amount Price Shipping
TA500398
  • CHEK2 (CHK2) mouse monoclonal antibody, clone OTI5C4 (formerly 5C4)
100ul 2500 现货 Add to Shopping Cart
TA500398S
  • CHEK2 (CHK2) mouse monoclonal antibody, clone OTI5C4 (formerly 5C4)
30ul850现货Add to Shopping Cart
CF500398
  • CHEK2 (CHK2) mouse monoclonal antibody, clone OTI5C4 (formerly 5C4)
100 ug4275.00现货Add to Shopping Cart
   TrueMABTM Antibodies
* Delivery is in business days
WB(3)
IP(1)
IHC(6)
IF(2)
FC(1)
Also for CHEK2 (Locus ID 11200)
Gene NameHomo sapiens checkpoint kinase 2 (CHEK2), transcript variant 1 Synonyms:CDS1; CHK2; hCds1; HuCds1; LFS2; PP1425; RAD53
ImmunogenFull length human recombinant protein of human CHEK2 (NP_009125) produced in HEK293T cell.
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone NameClone OTI5C4 IsotypeIgG1
Species ReactivityHuman , Dog Concentration1.43 mg/ml
Purification Purified from mouse ascites fluids by affinity chromatography
Guaranteed Application *WB, IHC, IF, IP, FC Suggested DilutionsWB 1:500~1000, IHC 1:150, IF 1:50~100, FLOW 1:100, IP 2ug/500ul
BackgroundIn response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Related Pathway
Cell cycle

* OriGene provides validated application data and protocol, with money back guarantee.


WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHEK2 (RC201278, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHEK2.
spacer

WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-CHEK2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
spacer

WB Image
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and CHEK2-Knockout hela cells (KO,Cat# LC810324) were separated by SDS-PAGE and immunoblotted with anti-CHEK2 monoclonal antibody TA500398. Then the blotted membrane was stripped and reprobed with anti-?-actin (TA811000) as a loading control.(1:500)
spacer

IHC Image
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500398)
spacer

IHC Image
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500398)
spacer

IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500398)
spacer

IHC Image
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500398)
spacer

IHC Image
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500398)
spacer

IHC Image
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500398)
spacer

IF Image
Anti-CHEK2 mouse monoclonal antibody (TA500398) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CHEK2(RC201278).
spacer

IF Image
Immunofluorescent staining of HT29 cells using anti-CHEK2 mouse monoclonal antibody (TA500398).
spacer

IP Image
Immunoprecipitation(IP) of CHEK2 by using TrueMab monoclonal anti-CHEK2 antibodies (Negative control: IP without adding anti-CHEK2 antibody.). For each experiment, 500ul of DDK tagged CHEK2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-CHEK2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
spacer

FC Image
Flow cytometric Analysis of Jurkat cells, using anti-CHEK2 antibody(TA500398),(Red), compared to a nonspecific negative control antibody,(Blue).
spacer



 

spacer
全国免费电话:400-6744-363(手机),800-9904-363(座机)订货邮箱:orderscn@origene.com,techsupport@origene.cn
电话:+86-10-59755312 传真:+86-10-59755322-888 地址:北京市经济技术开发区科创14街99号汇龙森科技园11栋
京ICP证07031721号 Copyright © 2017 北京傲锐东源(OriGene Technologies)生物科技有限公司,All Rights Reserved. 法律声明.