Chk1 (CHEK1) Mouse Monoclonal Antibody [Clone ID: 2G1D5]

Specifications

Product Data
Clone Name	2G1D5
Applications	ELISA, WB
Recommend Dilution	Western Blot: 1/500 - 1/2000. ELISA: 1/10000.
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant fragment of CHK1 expressed in E. Coli.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.03% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	56 kDa
Gene Name	checkpoint kinase 1
Database Link	Entrez Gene 1111 Human UniProt ID O14757
Background	CHK1, also known as CHEK1, it is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. Cdc25, when phosphorylated on serine 216 by chk1 becomes bound by an adaptor protein in the cytoplasm. Therefore it is inhibited from removing the inhibiting phosphate from MPF (mitotic/maturation promoting factor) added by Wee1. Consequently, a cell is prevented from entering mitosis. Chk1 can also phosphorylate p53 at Ser20 in vitro.
Synonyms	CHEK1, CHEK-1
Reference Data

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