Cyclin D1 (CCND1) Mouse Monoclonal Antibody [Clone ID: DCS-6]

Specifications

Product Data
Clone Name	DCS-6
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	ELISA and Biological Function Blockade: Use Antibody without BSA and Azide.  Western Blot: 5-10 µg/ml.  Immunohistochemistry on Frozen and Paraffin Sections: 0.5-1 µg/ml for 30 min at RT.  Immunohistochemical staining of formalin/paraffin tissues is enhanced by unmasking of the target epitope by boiling the tissue sections in 10mM citrate buffer, pH 6.0, for 10 min followed by cooling at room temp for 20 min.  Immunoprecipitation: 1-2 µg/500 µg protein lysate. Flow Cytometry: 0.5-1 µg/106 cells.  Immunofluorescence: 1-2 µg/ml.  Positive Control: T47D, ZR75, BT474, SKBR3, MCF-7, MDA-MB-453, HT29, Ramos, Jurkat or A431 cells. ~60% of mantle cell lymphomas and ~40% of breast carcinomas are positive.
Reactivity	Human, Monkey, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant full length Cyclin D1 protein.
Specificity	Recognizes a protein of 36kDa, identified as Cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of Human neoplasms. This antibody neutralizes the activity of Cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression of Cyclin D1. This antibody is useful in identifying mantle cell lymphomas (Cyclin D1 positive) from CLL/SLL and follicular lymphomas (Cyclin D1 negative). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1. Cellular Localization: Nuclear.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	36 kDa
Gene Name	cyclin D1
Database Link	Entrez Gene 595 Human UniProt ID P24385
Background	Cyclin D1 is a member of a superfamily of cyclins, proteins that govern transitions through distinct phases of the cell cycle by regulating CDKs. The D-type subfamily includes cyclin D1, D2, and D3. All three are differentially, and sometimes redundantly, expressed in different lineages. However, in every cell type at least one of the three can be detected. Cyclin D1 as well as D2 and D3 have central roles in linking exogenous growth regulating stimuli with the cycling machinery of cells. They are typically considered to be G1 cyclins; synthesis is initiated during G1 and their expression drives the G1/S phase transition. The deregulation of cyclin D1 or other D types is commonly involved in a wide range of cancers. For example cyclin D1 is activated by chromosomal rearrangements in both parathyroid adenomas and B-cell neoplasms.
Synonyms	Cyclin-D1, PRAD-1 oncogene, BCL-1 oncogene, CCND1, BCL1, PRAD1
Reference Data

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