ACTH (POMC) Mouse Monoclonal Antibody [Clone ID: AH26 + 57]

Specifications

Product Data
Clone Name	AH26 + 57
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	Flow Cytometry: 0.5-1 µg/million cells. Immunofluorescence: 0.5-1 µg/ml. Western Blotting: 0.5-1.0 µg/ml. Immunoprecipitation: 0.5-1 µg/500 µg protein lysate. Immunohistochemsitry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1.0 µg/ml for 30 min at RT.  No special pretreatment is required for staining of formalin-fixed tissues. Positive Control: Normal pituitary gland or pituitary tumor.
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide corresponding to aa1-24 of human ACTH (AH26); N-terminal fragment of human ACTH conjugated to KLH (57).
Specificity	ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This Monoclonal Antibody is specific to Synacthen (aa1-24 of ACTH); does not react with CLIP (aa17-39 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs). It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH. Cellular Localization: Cytoplasmic.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	ACTH is ~5 kDa, and the POMC precursor is ~30 kDa.
Gene Name	proopiomelanocortin
Database Link	Entrez Gene 5443 Human UniProt ID P01189
Synonyms	Pro-opiomelanocortin, Corticotropin-Lipotropin
Reference Data

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