Cortactin pTyr466 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	Western Blot: 1:500~1:1000.
Reactivity	Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	The antiserum was produced against synthesized phosphopeptide derived from mouse Cortactin around the phosphorylation site of tyrosine 466 (P-V-YP-E-T).
Specificity	Cortactin antibody detects endogenous levels of Cortactin only when phosphorylated at tyrosine 466.
Formulation	PBS(without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl, 0.02% sodium azide and 50% glycerol State: Aff - Purified State: Liquid purified IgG
Concentration	lot specific
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C. Avoid repeated freezing and thawing.
Gene Name	cortactin
Database Link	Entrez Gene 13043 Mouse UniProt ID Q60598
Background	Cortactin is an 80/85 kDa cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino terminal tandem repeats of a unique 37 amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an alpha helical structure followed by a proline rich region. Between the proline rich region and the SH3 domain, there are a series of Src phosphorylation sites (Tyr421, Tyr470, and Tyr486). In vitro, cortactin binds to and cross links F actin into meshworks.
Synonyms	CTTN, EMS1, Amplaxin, Oncogene EMS1
Reference Data

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