DNMT1 (C-term) Rabbit Polyclonal Antibody

CAT#: AP11047PU-N

DNMT1 (C-term) rabbit polyclonal antibody, Purified



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CNY 6,314.00


货期*
5周

规格
    • 400 ul

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Specifications

Product Data
Applications WB
Recommend Dilution ELISA: 1/1,000.
Western Blot: 1/100-1/500.
Reactivity Human
Host Rabbit
Clonality Polyclonal
Immunogen This Dnmt1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human Dnmt1.
Specificity This antibody reacts with Dnmt1 (C-term).
Formulation PBS containing 0.09% (W/V) Sodium Azide as preservative.
State: Purified
State: Liquid purified Ig fraction.
Concentration lot specific
Purification Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Conjugation Unconjugated
Storage Condition Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Gene Name DNA (cytosine-5-)-methyltransferase 1
Background Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2Fbinding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells.
Synonyms MCMT, HsaI, AIM, CXXC9
Note Calculated MW: 183164 Da
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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