RANKL (TNFSF11) Mouse Monoclonal Antibody [Clone ID: 2.1_3C12-2D11]

Specifications

Product Data
Clone Name	2.1_3C12-2D11
Applications	ELISA
Recommend Dilution	Sandwich ELISA: In a sandwich ELISA (assuming 100 µl/well), a concentration of 1.0-2.0 µg/ml of this antibody will detect at least 250 pg/ml of recombinant Human sRANK Ligand when used with biotinylated antigen affinity purified anti-human sRANK Ligand (Cat.-No PP1086B1 or PP1086B2) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	E.coli derived, 20.0 kDa Recombinant Human sRANK Ligand (Cat.-No PA122).
Specificity	This antibody reacts with Human sRANKL. Other species not tested.
Formulation	PBS without preservatives. State: Azide Free State: Lyophilized Sterile filtred, purified Ig fraction.
Reconstitution Method	Restore in sterile water to a concentration of 1.0 mg/ml.
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	tumor necrosis factor superfamily member 11
Database Link	Entrez Gene 8600 Human UniProt ID O14788
Background	RANKL is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. There are three isoforms of RANKL. Human RANKL is a soluble 20 kDa polypeptide, comprising the TNF homologous region of RANKL (176 amino acid residues). This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor associated factor (TRAF) 6, which indicated that this protein may have a role in the regulation of cell apoptosis. RANKL deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis.
Synonyms	OPGL, RANK Ligand, RANKL, TRANCE, TNFSF11, ODF
Reference Data

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