Isocitrate dehydrogenase (IDH1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, WB
Recommend Dilution	WB 1:500 - 1:2000;IF 1:50 - 1:200
Reactivity	Human, Mouse, Rat, Monkey
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant protein of human IDH1
Formulation	Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Concentration	lot specific
Purification	Affinity purification
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	isocitrate dehydrogenase (NADP(+)) 1, cytosolic
Database Link	NP_005887 Entrez Gene 15926 MouseEntrez Gene 24479 RatEntrez Gene 710019 MonkeyEntrez Gene 3417 Human UniProt ID O75874
Background	IDH1 is one of three isocitrate dehydrogenases that catalyze the oxidative decarboxylation of isocitrate to a-ketoglutarate (a-KG). These enzymes exist in two distinct subclasses that utilize either NAD or NADP+ respectively, as an electron acceptor. IDH1 is the NADP+-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. IDH2 and 3 are mitochodrial enzymes that also function in the Krebs cycle. IDH1 is inactivated by phosphorylation at Ser113 and contains a clasp-like domain wherein both polypeptide chains in the dimer interlock. IDH1 is expressed in a wide range of species and also in organisms that lack a complete citric acid cycle. Recently, an inactivating mutation of IDH1 has been implicated in glioblastoma. IDH1 appears to function as a tumor suppressor that, when mutationally inactivated, contributes to tumorigenesis in part through induction of the HIF-1 pathway.
Synonyms	HEL-216; HEL-S-26; IDCD; IDH; IDP; IDPC; PICD
Reference Data
Protein Pathways	Citrate cycle (TCA cycle), Glutathione metabolism, Metabolic pathways

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.