Chk2 (CHEK2) Mouse Monoclonal Antibody [Clone ID: 73C175.1.1]

Specifications

Product Data
Clone Name	73C175.1.1
Applications	FC, ICC/IF, IHC, WB
Recommend Dilution	Immunohistochemistry, Western Blot: 2 ug/ml, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence: 2-5 ug/ml, Flow Cytometry: 1-5 ug/ml
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	A portion of amino acids 100-150 of human Chk2 protein was used as immunogen for this antibody.
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	checkpoint kinase 2
Database Link	NP_009125 Entrez Gene 50883 MouseEntrez Gene 11200 Human UniProt ID O96017
Background	Chk2 (checkpoint kinase 2) is the mammalian homolog of the Saccharomyces cerevisiae Rad53 and is required for the DNA damage and replication checkpoints (1). In response to low-dose ionizing radiation (IR), which occurs in an ataxia telangiectasia mutated (ATM)-dependent manner, Chk2 can phosphorylate the mitosis-inducing phosphatase Cdc25C on an inhibitory site, blocking entry into mitosis, and p53 on a regulatory site, causing G1 arrest (2,3). In human tissues, Chk2 is homogeneously expressed in renewing cell populations such as epidermis or intestine, heterogeneous in conditionally renewing tissues, and absent or at low level in static tissues such as muscle or brain (4). Mutations in Chk2 inactivates DNA damage checkpoint pathway involving Chk2 in lung cancer suggesting that reduced expression of Chk2 may be responsible for the development of lung cancer (5). The Chk2-/- knockout cells are resistant to DNA damage-induced apoptosis, defective for p53 stabilization and for induction of p53-dependent transcripts such as p21 in response to gamma irradiation (6).
Synonyms	CDS1; CHK2; hCds1; HuCds1; LFS2; PP1425; RAD53
Reference Data
Protein Families	Druggable Genome, Protein Kinase, Stem cell - Pluripotency
Protein Pathways	Cell cycle, p53 signaling pathway

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