Mgmt (NM_008598) Mouse Untagged Clone

Name: Mgmt (NM_008598) Mouse Untagged Clone
SKU: MC202089
Price: 2400.00

CAT＃: MC202089

Mgmt (untagged) - Mouse O-6-methylguanine-DNA methyltransferase (Mgmt), (10ug)

Datasheet

SDS

"NM_008598" in other vectors (6)

CNY 2,000.00

货期*

现货

规格

Cited in 1 publication.

Product images

经常一起买 (3)

TF81001

TurboFectin Transfection Reagent (1 mL in 1 vial)

CNY 4,090.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Agat; AGT; AI267024
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC031888 sequence for NM_008598 AAAACTTGTGTACCGTTCCCCGTTGCTGTCTGCAGTTTGCAAGCTGGAACTTGGCAGAATGGCTGAGACC TGCAAAATGAAATACTCAGTGTTGGACAGCCCTTTGGGGAAGATGGAGCTGTCTGGCTGTGAGCGAGGCC TGCATGGGATACGGTTGCTCAGTGGGAAGACCCCAAACACTGACCCCACAGAGGCCCCAGCTACTCCTGA GGTGCTCGGTGGGCCAGAGGGAGTTCCAGAGCCTCTGGTGCAGTGCACAGCCTGGCTGGAAGCCTATTTC CGTGAACCCGCAGCCACAGAGGGGCTTCCCTTGCCTGCTCTCCATCACCCTGTGTTCCAGCAAGATTCAT TCACCAGACAGGTGTTATGGAAGCTGCTGAAGGTTGTGAAATTCGGAGAAACGGTTTCTTACCAGCAATT AGCAGCCCTGGCAGGCAACCCCAAAGCGGCTCGTGCAGTAGGAGGAGCAATGAGAAGCAATCCGGTCCCC ATCCTCATCCCCTGCCACAGGGTGGTTCGCAGTGACGGTGCCATCGGCCATTACTCCGGAGGAGGGCAGG CTGTGAAGGAGTGGCTTCTGGCCCATGAGGGCATCCCGACCGGACAGCCAGCCTCCAAGGGCTTGGGTCT GACTGGGACCTGGCTCAAGTCATCCTTCGAGTCGACCAGCTCTGAGCCGTCTGGCCGAAATTGAGTAACC GTTTGAATGACACATAGATGTAACGCGTGTCGGAAGCGGATGTGTGGTGGCACCACTATATTAAAAGAGC TGCAAGTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	NM_008598
Insert Size	636 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC031888, AAH31888
RefSeq Size	815 bp
RefSeq ORF	636 bp
Locus ID	17314
UniProt ID	P26187
Gene Summary	Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

The use of this cDNA Clones has been cited in the following citations:
Coordinated Splicing of Regulatory Detained Introns within Oncogenic Transcripts Creates an Exploitable Vulnerability in Malignant Glioma ,Braun, CJ;Stanciu, M;Boutz, PL;Patterson, JC;Calligaris, D;Higuchi, F;Neupane, R;Fenoglio, S;Cahill, DP;Wakimoto, H;Agar, NYR;Yaffe, MB;Sharp, PA;Hemann, MT;Lees, JA;, Cancer Cell ,PubMed ID 28966034 [MGMT]

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
MG202297	Mgmt (tGFP-tagged) - Mouse O-6-methylguanine-DNA methyltransferase (Mgmt)	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,370.00
MR202297	Mgmt (Myc-DDK-tagged) - Mouse O-6-methylguanine-DNA methyltransferase (Mgmt)	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,600.00
MR202297L1	Lenti ORF clone of Mgmt (Myc-DDK-tagged) - Mouse O-6-methylguanine-DNA methyltransferase (Mgmt)	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
MR202297L2	Lenti ORF clone of Mgmt (mGFP-tagged) - Mouse O-6-methylguanine-DNA methyltransferase (Mgmt)	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,000.00
MR202297L3	Lenti ORF clone of Mgmt (Myc-DDK-tagged) - Mouse O-6-methylguanine-DNA methyltransferase (Mgmt)	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,000.00
MR202297L4	Lenti ORF clone of Mgmt (mGFP-tagged) - Mouse O-6-methylguanine-DNA methyltransferase (Mgmt)	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00

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Proteins

Antibodies