Kdm5a (NM_145997) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AA409370; C76986; Jarid1a; Rbbp2; RBP2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224835 representing NM_145997 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCCGTGGGCCCGGGGGGCTACGCGGCGGAGTTCGTGCCACCGCCCGAGTGCCCGGTCTTTGAGC CCAGTTGGGAGGAGTTCACAGACCCGCTGAGTTTTATCGGGCGCATCCGGCCCTTTGCGGAGAAAACGGG CATCTGCAAAATCCGGCCACCCAAGGATTGGCAACCTCCATTTGCCTGTGAAGTAAAAACCTTCCGTTTC ACTCCTAGAGTCCAGCGCCTGAATGAACTTGAGGCAATGACTAGAGTAAGATTGGACTTCTTGGATCAAC TGGCAAAATTTTGGGAACTTCAAGGATCTACATTGAAGATCCCTGTGGTGGAGAGAAAAATCCTGGATTT GTATGCTTTAAGCAAGATTGTTGCCAGCAAAGGAGGTTTTGAAATAGTCACCAAAGAGAAGAAATGGTCT AAAGTGGGTAGCCGCTTGGGATATCTGCCAGGAAAAGGAACTGGGTCTCTTTTGAAGTCACACTATGAAA GAATTCTCTACCCATATGAACTTTTCCAATCTGGTGTCAGCCTTATGGGTGTACAAATGCCTGATTTAGA TCTTAAAGAAAAAGTTGAGGCTGAGGTTCTCAGCACTGACATCCAGCCTTCCCCAGAGCGGGGAACAAGA ATGAATATTCCACCAAAGAGAACAAGACGAGTTAAGTCTCAGTCAGACTCTGGAGAAGTAAATAGAAACA CAGAACTGAAGAAACTTCAGATTTTTGGGGCTGGACCCAAAGTTGTGGGCCTGGCAGTAGGAGCAAAAGA TAAAGAAGATGAAGTCACCCGAAGAAGAAAAGTTACCAACAGGTCAGATGCATTTAACATGCAAATGAGA CAACGGAAAGGAACTCTCTCTGTTAACTTTGTTGATCTCTATGTTTGCATGTTTTGTGGTCGAGGAAATA ATGAGGATAAACTGCTTTTGTGTGATGGGTGCGATGACAGCTATCATACATTTTGTCTACTTCCACCACT ACCTGATGTCCCCAAAGGAGACTGGAGGTGTCCTAAATGTGTTGCTGAGGAATGTAACAAACCTCGAGAA GCCTTTGGATTTGAGCAAGCTGTAAGAGAATATACACTTCAAAGCTTTGGAGAGATGGCAGATAATTTTA AATCTGATTATTTCAATATGCCAGTCCATATGGTTCCTACAGAGCTAGTAGAAAAAGAATTCTGGCGGCT AGTAAGTAGCATTGAAGAAGATGTTATTGTAGAGTATGGAGCTGATATCTCTTCAAAAGATTTTGGAAGT GGATTTCCTAAAAAGGATGGTCAAAGAAAGATGTTGCCAGAAGAAGAGGAATATGCACTTTCTGGTTGGA ATTTGAATAACATGCCTGTTCTGGAGCAGTCCGTTCTTGCACATATTAATGTGGACATTTCTGGTATGAA GGTGCCATGGCTCTATGTGGGAATGTGCTTTTCTTCTTTTTGCTGGCATATTGAAGATCACTGGAGTTAT TCCATTAACTACTTGCATTGGGGCGAGCCAAAGACATGGTATGGTGTACCATCTCATGCTGCAGAACAAC TGGAGGAGGTGATGAGGGAGCTGGCCCCTGAGTTATTTGAATCCCAGCCTGATCTCCTACATCAGTTAGT AACCATCATGAACCCAAATGTGCTCATGGAGCATGGTGTGCCTGTGTACAGGACTAATCAGTGTGCTGGA GAGTTTGTGGTGACGTTTCCTCGTGCCTATCACTCTGGATTTAACCAGGGATACAACTTTGCCGAAGCGG TGAACTTCTGTACTGCTGACTGGTTGCCCATTGGACGTCAGTGTGTAAATCACTATCGACGGCTCAGGCG CCATTGTGTCTTTTCACATGAGGAACTCATTTTCAAGATGGCAGCAGATCCAGAATGCTTAGATGTGGGG CTGGCTGCCATGGTATGCAAAGAGTTGACTCTCATGACTGAAGAAGAAACACGATTGAGAGAATCAGTTG TTCAAATGGGTGTCGTGATGTCAGAAGAAGAAGTCTTTGAACTTGTTCCTGATGATGAGCGGCAGTGTTC AGCATGCAGAACTACATGTTTTCTCTCAGCTCTCACATGCTCCTGTAATCCGGAGCGACTTGTATGTCTG TATCATCCAACTGATTTGTGCTCTTGCCCCATGCAGAACAAATGTCTTAGGTACCGCTATCCACTAGAAG ACCTTCCATCTCTGCTTTATGGTGTCAAAGTGAGAGCACAATCCTATGACACTTGGGTCAATCGTGTTAC AGAAGCATTGTCAGCTAGCTTCAACCATAAGAAAGATTTGATTGAATTGAGAGTAATGCTGGAAGATGCT GAGGATAGGAAATACCCAGAGAATGATCTCTTTCGAAAGCTCAGGGACGCGGTGAAAGAAGCTGAGACCT GTGGTTCTGTGGCTCAGCTGCTTCTGAGCAAAAAGCAAAAACACAGGCAGAGCTCAGATAGTGGGAAGAC TCGGACCAAACTGACAGTAGAAGAATTGAAGGCCTTTGTCCAACAACTTGTTAGTCTTCCGTGTGTCATC AGCCAAACTCGACAAGTAAAGAATCTGCTGGATGATGTAGAAGAATTTCATGAACGTGCCCAGGAGGCCA TGATGGATGAAACCCCAGATTCTTCCAAACTCCAAATGTTAATAGACATGGGCTCTAGTCTCTATGTGGA GCTACCTGAATTGCCCCGACTAAAGCAAGAGCTCCAGCAGGCTCGATGGCTGGATGAAGTAAGGCTAACT CTATCAGATCCACAACAAGTCACTTTGGATGTCATGAAGAAATTGATTGACTCTGGCGTAGGCTTGGCAC CTCACCATGCTGTAGAAAAAGCAATGGCCGAACTACAGGAACTCCTTACAGTCTCTGAACGATGGGAAGA GAAGGCTAAGGTCTGCCTGCAAGCAAGACCTCGGCACAGTATGGCAAATTTAGAAAATATTGTGAATGAA GCCAAGAACATTCCAGCCTTTCTACCCAATGTGTTGTCCTTAAAAGAAGCCTTACAAAAGGCTCGTGAAT GGACAGCTAAAGTGGAAGCTATTCAGAGTGGCAACAACTATGCTTACTTGGAACAGCTTGAGAGCCTATC TGCTAAAGGACGCCCTATCCCTGTGCGTCTTGATGCCTTGCCTCAAGTGGAGTCACAGGTAGCAGCAGCA CGGGCCTGGAGAGAACGTACTGGGCGGACCTTCCTTAAAAAGAATTCCAGCCATACATTGTTACAGGTGC TAAGTCCACGAACTGACATTGGTGTATATGGGAGTGGTAAAAACAGGAGGAAAAAGGTAAAAGAAATAAT AGAAAAAGAAAAGGAGAAGGACCTCGACCTTGAGCCTTTGAGTGACTTAGAGGAAGGACTGGAGGAGTCA AGAGATACAGCCATGGTGGTGGCAGTTTTCAAAGAACGAGAGCAAAAGGAGATTGAAGCCATGCATTCCC TCAGAGCAGCCAACCTAGCCAAGATGACAATTGTGGAGCGCATAGAAGAAGTGAAATTTTGTATTTGCCG CAAGACAGCCAGTGGGTTTATGCTACAGTGTGAGCTCTGCAAAGACTGGTTCCATAACAGCTGTGTTCCC CTTCCTAAATCAAGTTCCCAGAAAAAGGGATCCAGCTGGCAGGCTAAAGATGTAAAATTCCTTTGCCCTC TTTGCATGAGATCTCGGAGGCCAAGGCTAGAGACTATTCTGTCACTCCTGGTATCCCTTCAGAAGTTGCC TGTACGGTTGCCGGAAGGAGAAGCACTGCAGTGTTTGACAGAACGTGCCATGAGTTGGCAAGATAAAGCA CGCCAAGCTTTAGCCACAGATGAACTGTCCTCTGCCCTGGCCAAGCTCTCTGTGCTAAGCCAGCGTATGG TGGAACAAGCAGCTAGAGAAAAAACTGAAAAGATTATCAGTGCAGAACTCCAAAAAGCAGCTGCCAATCC AGATTTACAGGGACACTTACCTAGTTTCCAGCAGTCTGCTTTTAACCGGGTGGTGAGCAGTGTATCATCT TCCCCCCACCAAACAATGGACTATGATGATGAAGAAACAGATTCTGATGAAGATATTCGGGAGACATACG GCTATGACATGAAGGATACAGCCAGCGTGAAGTCCTCTAGTAGTCTGGAACCCAATCTCTTTTGTGATGA AGAGATTCCCATCAAGTCTGAGGAAGTGGTGACTCACATGTGGACGGCACCGTCATTCTGTGCAGAGCAT GCATATTCTTCTGCTTCAAAGAGTTGTTCTCAAGGTTCTAGCACTCCAAGAAAGCAGCCTCGGAAGAGCC CTTTGGTTCCCAGAAGTTTGGAGCCTCCAGTATTGGAATTGTCTCCTGGAGCAAAAGCCCAGTTGGAAGA ACTCATGATGGTTGGAGACCTCCTAGAAGTGTCTCTGGATGAGACACAACACATTTGGCGGATTTTGCAG GCCACACATCCACCCTCAGAGGACAGATTCTTACATATCATGGAGGATGATAGCATAGAAGAGAAACCAT TAAAAATGAAAGGAAAGGACTCTTCAGAGAAGAAACGGAAACGGAAGCTAGAAAAGGTAGAACAGCTTTT TGGAGAAGGAAAACAGAAGACCAAGGAGCTAAAGAAAATAGATAAACCTAAAAAGAAGAAATTAAAATTA AATGTGGACAAATCAAAAGAGCTGAATAAATTGGCCAAGAAGTTAGCTAAAGAAGAAGAGCGAAAGAAAA AGAAAGAAAAGGCTGCTGCCGCCAAAGTGGAGCTTGTGAAAGAGAGCACGGAGAAGAAACGGGAGAGGAA GGTGTTGGATATCCCCTCAAAGTATGACTGGTCGGGGGCAGAGGAGTCTGATGATGAGAATGCTGTGTGT GCAGCCCAAAACTGCCAAAGGCCGTGTAAGGACAAGGTAGACTGGGTACAGTGTGATGGTGGCTGTGATG AGTGGTTTCATCAAGTTTGTGTTGGTGTATCCGCAGAAATGGCTGAAAATGAAGATTACATCTGTATAAA CTGTGCAAAGAAGCAGGGGCCAGATAGCCCAGGCCAAGCACCACCTCCTCCCTTCCTAATGAGCTACAAA CTACCAATGGAGGATCTTAAAGAGACCAGTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_145997
Insert Size	5073 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_145997.2, NP_666109.2
RefSeq Size	10969 bp
RefSeq ORF	5073 bp
Locus ID	214899
UniProt ID	Q3UXZ9
Gene Summary	Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4' (PubMed:17320161, PubMed:17320163). Regulates specific gene transcription through DNA-binding on 5'-CCGCCC-3' motif. May stimulate transcription mediated by nuclear receptors. Involved in transcriptional regulation of Hox proteins during cell differentiation (By similarity). May participate in transcriptional repression of cytokines such as CXCL12. Plays a role in the regulation of the circadian rhythm and in maintaining the normal periodicity of the circadian clock. In a histone demethylase-independent manner, acts as a coactivator of the CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER1/2 and other clock-controlled genes and increases histone acetylation at PER1/2 promoters by inhibiting the activity of HDAC1 (PubMed:21960634). Seems to act as a transcriptional corepressor for some genes such as MT1F and to favor the proliferation of cancer cells (By similarity).[UniProtKB/Swiss-Prot Function]

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