Def8 (NM_001253783) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI449518; D8Ertd713e; DEF-8
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227866 representing NM_001253783 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGGTGAAAGGGGAACATCTCAAGTAAGGGGACAGGAAGGAAGATCCAGTTCCAAGAGGTGGGATG CTATGGAATATGATGAGAAGCTGGTTCGGTTCCGGCAGGCCCACCTCAACCCCTTCAACAAGCAGCTTGG TCCGAGGCATCATGAACAGGAACCCAGTGAGAAGGTCACTTCTGAAGACACTTTGCCTGAGCTGCCCGCT GGGGAGCCTGAATTCCACTACTCGGAGCGCATGATGGATCTCGGCCTGTCTGAGGACCACTTTTCCCGCC CTGTGGGTCTCTTCCTGGCCTCTGATGTCCAGCAGCTGCGGCAGGCCATCGAAGAATGCAAACAGGTGAT CCTGGAGCTGCCCGAGCAGTCAGAGAAGCAGAAGGACGCTGTGGTGCGGCTGATCCACCTCCGGCTGAAG CTCCAGGAGCTGAAGGACCCCAATGAGGAGGAACCCAACATCCGGGTTCTCCTGGAACATCGCTTCTACA AGGAGAAGAGCAAGAGCGTTAAACAGACCTGCGATAAGTGCAACACCATCATCTGGGGGCTCATCCAGAC CTGGTACACCTGCACAGGGTGTTGTTACCGCTGTCACAGCAAGTGCCTGAACCTCATCTCCAAACCATGT GTCAGCTCCAAGGTCAGTCACCAGGCTGAGTATGAGCTGAACATCTGCCCTGAGACCGGGCTGGACAGCC AGGACTACCGCTGTGCGGAGTGCCGTGCTCCCATCTCTCTGAGAGGTGTGCCTAGTGAGGCCCGGCAGTG TGACTACACGGGCCAATACTACTGCAGCCACTGCCACTGGAACGACCTGGCTGTCATCCCCGCGAGAGTG GTGCACAACTGGGACTTTGAGCCACGCAAGGTGTCCCGTTGCAGCATGCGCTACCTGGCACTGATGGTGT CTCGGCCAGTGCTCCGGCTCCGGGAGATTAACCCTTTGCTGTTTAACTACGTGGAAGAGCTGGTGGAGAT CCGGAAACTGCGCCAGGACATCCTGCTCATGAAGCCATACTTCATCACCTGCAAGGAGGCCATGGAGGCG CGACTACTGCTGCAGCTCCAAGACAGACAGCATTTTGTGGAGAACGATGAGATGTACTCTATCCAGGACC TCTTGGAAGTGCACATGGGCCGCCTCAGCTGCTCGCTCACTGAGATCCACACGCTCTTCGCCAAGCACAT CAAGTTGGACTGTGAGCGGTGCCAAGCCAAGGGGTTCGTCTGTGAACTCTGCAAAGAAGGGGACGTGCTC TTCCCGTTCGACAGCCACACGTCTGTGTGCAATGACTGTTCGGCTGTCTTCCACAGGGACTGTTACTACG ACAACTCGACCACGTGCCCCAAGTGTGCCCGGCTCACATTGAGGAAGCAGTCACTATTCCAGGAACCTGG CCTAGACATGGATGCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001253783
Insert Size	1419 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001253783.1, NP_001240712.1
RefSeq Size	3895 bp
RefSeq ORF	1419 bp
Locus ID	23854
UniProt ID	Q99J78
Gene Summary	Positively regulates lysosome peripheral distribution and ruffled border formation in osteoclasts (PubMed:27777970). Involved in bone resorption (PubMed:27777970).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest protein (isoform 1).

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