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Dnajb1 (NM_018808) Mouse Tagged ORF Clone
CAT＃: MG205083
- TrueORF®
Dnajb1 (tGFP-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily B, member 1 (Dnajb1)
ORF Plasmid: DDK
"NM_018808" in other vectors (4)
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CNY 3,710.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 0610007I11Rik; DjB1; Hdj1; Hsp; Hsp40; HSPF1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG205083 representing NM_018808
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAAGGACTACTATCAGACGCTGGGCCTGGCCCGCGGCGCGTCCGACGACGAGATCAAGCGAGCCT ACCGCCGCCAGGCGCTGCGCTACCACCCGGACAAGAACAAGGAGCCCGGCGCTGAGGAGAAGTTCAAGGA GATCGCCGAGGCCTACGACGTGCTCAGCGACCCGCGCAAGCGCGAGATTTTCGACCGCTATGGAGAGGAA GGCCTGAAGGGTGGTAGCCCCAGTGGAGGAAGCAGTGGTGGTGCTAATGGTACCTCTTTCAGCTACACAT TCCACGGAGACCCCCATGCCATGTTTGCTGAGTTCTTCGGTGGCAGAAACCCCTTTGATACCTTTTTTGG GCAGCGCAACGGGGAAGAAGGCATGGACATTGATGACACATTCTCTAGCTTTCCAATGGGTATGGGTGGC TTCACCAACATGAACTTTGGACGTTCCCGCCCTTCTCAAGAGCCCACCCGGAAGAAGCAAGATCCTCCAG TCACCCATGACCTTCGGGTCTCCCTTGAAGAGATCTACAGCGGCTGTACCAAGAAGATGAAAATCTCCCA CAAGCGGCTGAACCCTGATGGAAAGAGCATTCGAAATGAAGATAAGATCCTGACCATCGAAGTGAAGAGG GGCTGGAAAGAAGGGACCAAAATCACCTTTCCCAAGGAAGGGGACCAGACCTCGAACAACATTCCAGCAG ACATCGTCTTTGTTTTAAAGGACAAGCCACACAATATCTTCAAGAGAGATGGTTCTGATGTCATCTATCC AGCCAGGATTAGCCTTCGGGAGGCTCTCTGTGGTTGCACTGTGAATGTCCCTACTCTGGACGGCAGGACC ATCCCTGTTGTATTCAAAGATGTCATCAGGCCTGGTATGCGGCGGAAAGTCCCTGGAGAAGGCCTCCCTC TCCCCAAAACACCTGAGAAACGTGGAGACCTTGTTATCGAGTTTGAAGTGATCTTCCCGGAAAGGATTCC CGTCTCATCCAGAACCATCCTGGAGCAGGTTCTTCCCATA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205083 representing NM_018808
Red=Cloning site Green=Tags(s) MGKDYYQTLGLARGASDDEIKRAYRRQALRYHPDKNKEPGAEEKFKEIAEAYDVLSDPRKREIFDRYGEE GLKGGSPSGGSSGGANGTSFSYTFHGDPHAMFAEFFGGRNPFDTFFGQRNGEEGMDIDDTFSSFPMGMGG FTNMNFGRSRPSQEPTRKKQDPPVTHDLRVSLEEIYSGCTKKMKISHKRLNPDGKSIRNEDKILTIEVKR GWKEGTKITFPKEGDQTSNNIPADIVFVLKDKPHNIFKRDGSDVIYPARISLREALCGCTVNVPTLDGRT IPVVFKDVIRPGMRRKVPGEGLPLPKTPEKRGDLVIEFEVIFPERIPVSSRTILEQVLPI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018808 |
| ORF Size | 1020 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018808.3 |
| RefSeq Size | 2279 bp |
| RefSeq ORF | 1023 bp |
| Locus ID | 81489 |
| UniProt ID | Q9QYJ3 |
| Gene Summary | This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. The encoded protein may also inhibit apoptosis. Peritoneal macrophages derived from homozygous knockout mice for this gene exhibit impaired heat tolerance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200830 | Dnajb1 (untagged) - Mouse DnaJ (Hsp40) homolog, subfamily B, member 1 (Dnajb1), (10ug) |
CNY 2,000.00 |
|
| MR205083 | Dnajb1 (Myc-DDK-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily B, member 1 (Dnajb1) |
CNY 3,330.00 |
|
| MR205083L3 | Lenti ORF clone of Dnajb1 (Myc-DDK-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily B, member 1 (Dnajb1) |
CNY 5,230.00 |
|
| MR205083L4 | Lenti ORF clone of Dnajb1 (mGFP-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily B, member 1 (Dnajb1) |
CNY 5,230.00 |
