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Ttf1 (NM_009442) Mouse Tagged ORF Clone
CAT＃: MG210876
- TrueORF®
Ttf1 (tGFP-tagged) - Mouse transcription termination factor 1 (Ttf1)
ORF Plasmid: DDK
"NM_009442" in other vectors (4)
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CNY 11,048.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AV245725; TTF-1; TTF-I |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210876 representing NM_009442
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGGGGGCACAAGCAAATTTAAGACCCACACTGAAACTTTGTACAAGAAAAAGAAATGGTCTTCTG TGTCTGAAAAAAGACCTCAGAAATGTCCCTCTCAGTGCTTGGAGAGCAAGCAGCCTCAGGTATCTGTGTT GGGGAAGAGGAGGAGGGCGTCCCAGACACCAGCCCAGGAGACCTTAGAGAGTGAGTGGCCTCAAAAGGCC AAGAGGAAGAAGAGGAGGAGGGAACCCCAGACACCAGCCCAGGAGACCTTAGAGAGTGAGTGGCCTCAAA AGGCCAAGAAGAAGAAGAGAAGGGGGGAACCCCAGACACCAACCCAAGAGAGTTTGGAGAGTGAGCAGCC TCCAGTGTCCTTGCTGGGGAAGAGAAGGAGGGAATCCCAGACACCAGCCCAGGAGAACTCGGAGAGTGAG CAGCCTCGGAAAGCCAAGAGAAGGAGGAAGAAAAGGAAAGGGTCCCAGCAACCAACTTCCTCCCTTCTGA AAACTCCAGAAACCTTTCTTAAAGCTAAGAAAACCACTTCTGCCCACAAGAAGAAAAAGAATAGTGTTCT GGAGGTGGATATGGAAACTGGGATCATCCTTGTAGATAAAGAAAACATGGAGAACCTGCTAGAGACTTCT AGAAAGGATGTGGATATTGTTTATGTTGATATGAGCAAGGGACAAAGGTCAGCAAAAGTGCGTGAAACAG GAGAGCTGCCCGCTGCTAAGCCACAGGAACATGGCTGTCGAGAGCTGCTCGGTGACGTCAGGAGCAGAAA GAAACAAAAGCACCTCCAGAAAGTTGCACCCTGGGATGTTGTTCAGGGGAGCCAGCCTGAGAGTATCTCC CTGCCCCCATCAGAGCCCTTGTCTTCTGAAGATTTAGAAGGCAAAAGCACAGAAGCAGCAGTGTTTTGTA AAAAGAAGTCTAAAAAAAACGTGTTCAGGAGCCAGGAATTGGAGCCCATCCCCGACAGTCTTGATGACTC GGAGACCATCTCTGAGAGACTTGATAGCACACACCATGGAGGAGCTGTCGGGGCTGGAGAAGAATGTGAG AGCACCAAAGAATCCCACAGTATCAAAAAAAAGTCCAAGAAAAAGAAGCACAAGTCTGTTGCTCTTGCCA CATCTAGTGACAGTGCCTCAGTGACAGACAGCAAGGCTAAGAATGCCCTGGTGGACTCCTCAGAAGGCAG TGGTGCTGTGAGGGAAGAGGACGTGGACCACAGGCCAGCAGAGGCAGAAGCCCAGGCTTGTTCCACTGAG AAGCACAGGGAAGCCATGCAGAGGTTAGAACCTACCCATGAAGAAGAAAGCAATTCGGAATCAGCTAGCA ATTCTGCAGCAAGACACATATCTGAGGACAGGAGAGAGTCTGATGACTCGGATGTTGATTTGGGCTCTGC AGTGAGACAGCTGCGAGAGTTCATTCCTGACATACAGGAAAGGGCGGCCACCACCATCAGACGCATGTAT CGGGATGACCTAGGGCGCTTCAAAGAATTTAAGGCACAAGGTGTGGCTATTAGATTTGGCAAATTTTCTG CTAAAGAAAATAAACAAATAGAAAAAAATGTGCAAGATTTCCTATCCCTGACTGGAATTGAGAGTGCAGA CAAGCTGCTGTACACAGACAGATACCCAGAGGAAAAGACTCTGATCACCAACCTAAAACGGAAGCATGCC TTCAGACTGCACATTGGGAAGGGCATCGCTCGGCCCTGGAAGCTCGTGTACTACCGTGCAAAGAAGATCT TTGATGTGAATAACTACAAAGGCAGGTACAATGAAGAAGATACTAAGAAGCTGAAGGCATACCATTCCCT CCATGGAAACGACTGGAAAAAGATTGGGGCCATGGTGGCCCGAAGCAGCCTCTCAGTCGCCCTCAAGTTC TCTCAGATTGGTGGTACTAGAAACCAAGGTGCTTGGAGTAAGGCAGAAACCCAGAGGCTAATCAAGGCTG TGGAGGACGTGATCCTAAAGAAGATGTCTCCCCAGGAGTTAAGAGAACTGGATTCTAAACTCCAGGAAGA CCCTGAGGGTCGCCTGTCAATTGTCCGGGAAAAACTCTACAAGGGCATATCTTGGGTGGAGGTGGAAGCT AGAGTGGAGACCCGGAACTGGATGCAGTGCAAAAGTAAGTGGACAGAGATTCTTACCAAAAGGATGACCC ATGGTGGGTTCGTGTATCGTGGGGTCAATGCTCTACAGGCCAAAATCACCCTTATTGAAAGGTTGTATGA ACTAAATGTGAATGATGCTAATGAAATAGACTGGGAAGATCTTGCTAGCGCCATAGGGGATGTCCCTCCA CCTTTTGTTCAGGCAAAATTTTATAAGCTGAAAGCTGCCTGCGTTCCCTTTTGGCAGAAAAAGACTTTTC CAGAAATCATTGACTACTTATATAAGAACAGTCTGCCTTTGCTGAAGGAAAAGTTAGACAAGAAAATGAA GAAAAAAGACGGCCAGATCCAAACACCTGCAGCTCCCAAACAAGACTTTCTGTTCAAAGACATCTTCCAT TGCGATGATGACAGTGATGAAGGGAGCCCAGAGGAGCCCAGCGCCTCTGATGTGCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210876 representing NM_009442
Red=Cloning site Green=Tags(s) MKGGTSKFKTHTETLYKKKKWSSVSEKRPQKCPSQCLESKQPQVSVLGKRRRASQTPAQETLESEWPQKA KRKKRRREPQTPAQETLESEWPQKAKKKKRRGEPQTPTQESLESEQPPVSLLGKRRRESQTPAQENSESE QPRKAKRRRKKRKGSQQPTSSLLKTPETFLKAKKTTSAHKKKKNSVLEVDMETGIILVDKENMENLLETS RKDVDIVYVDMSKGQRSAKVRETGELPAAKPQEHGCRELLGDVRSRKKQKHLQKVAPWDVVQGSQPESIS LPPSEPLSSEDLEGKSTEAAVFCKKKSKKNVFRSQELEPIPDSLDDSETISERLDSTHHGGAVGAGEECE STKESHSIKKKSKKKKHKSVALATSSDSASVTDSKAKNALVDSSEGSGAVREEDVDHRPAEAEAQACSTE KHREAMQRLEPTHEEESNSESASNSAARHISEDRRESDDSDVDLGSAVRQLREFIPDIQERAATTIRRMY RDDLGRFKEFKAQGVAIRFGKFSAKENKQIEKNVQDFLSLTGIESADKLLYTDRYPEEKTLITNLKRKHA FRLHIGKGIARPWKLVYYRAKKIFDVNNYKGRYNEEDTKKLKAYHSLHGNDWKKIGAMVARSSLSVALKF SQIGGTRNQGAWSKAETQRLIKAVEDVILKKMSPQELRELDSKLQEDPEGRLSIVREKLYKGISWVEVEA RVETRNWMQCKSKWTEILTKRMTHGGFVYRGVNALQAKITLIERLYELNVNDANEIDWEDLASAIGDVPP PFVQAKFYKLKAACVPFWQKKTFPEIIDYLYKNSLPLLKEKLDKKMKKKDGQIQTPAAPKQDFLFKDIFH CDDDSDEGSPEEPSASDVQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009442 |
| ORF Size | 2577 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009442.2, NP_033468.2 |
| RefSeq Size | 4170 bp |
| RefSeq ORF | 2580 bp |
| Locus ID | 22130 |
| UniProt ID | Q62187 |
| Gene Summary | Multifunctional nucleolar protein that terminates ribosomal gene transcription, mediates replication fork arrest and regulates RNA polymerase I transcription on chromatin. Plays a dual role in rDNA regulation, being involved in both activation and silencing of rDNA transcription. Interaction with BAZ2A/TIP5 recovers DNA-binding activity.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222135 | Ttf1 (untagged) - Mouse transcription termination factor, RNA polymerase I (Ttf1), (10ug) |
CNY 10,930.00 |
|
| MR210876 | Ttf1 (Myc-DDK-tagged) - Mouse transcription termination factor, RNA polymerase I (Ttf1) |
CNY 9,448.00 |
|
| MR210876L3 | Lenti ORF clone of Ttf1 (Myc-DDK-tagged) - Mouse transcription termination factor, RNA polymerase I (Ttf1) |
CNY 10,450.00 |
|
| MR210876L4 | Lenti ORF clone of Ttf1 (mGFP-tagged) - Mouse transcription termination factor, RNA polymerase I (Ttf1) |
CNY 10,450.00 |
