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Cltc (NM_001003908) Mouse Tagged ORF Clone
CAT＃: MG212064
- TrueORF®
Cltc (tGFP-tagged) - Mouse clathrin, heavy polypeptide (Hc) (Cltc)
ORF Plasmid: DDK
"NM_001003908" in other vectors (2)
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CNY 15,580.00
货期*
3周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 3110065L21Rik; CHC; R74732 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG212064 representing NM_001003908
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCAGATTCTGCCCATTCGCTTTCAGGAGCATCTCCAGCTCCAGAACCTGGGTATCAACCCAGCAA ACATTGGCTTCAGTACCTTGACTATGGAGTCAGACAAATTCATCTGCATTAGAGAAAAAGTGGGAGAGCA GGCCCAGGTGGTAATCATTGATATGAATGATCCAAGTAATCCAATTCGAAGACCAATCTCAGCAGACAGT GCCATCATGAATCCTGCCAGCAAAGTGATTGCACTGAAAGCTGGCAAAACTCTTCAGATATTTAACATTG AAATGAAAAGTAAAATGAAGGCCCATACCATGACTGATGATGTCACCTTCTGGAAATGGATCTCTTTGAA TACGGTTGCTCTTGTTACGGATAATGCAGTTTATCACTGGAGTATGGAAGGAGAGTCTCAGCCAGTGAAA ATGTTTGATCGTCATTCTAGCCTCGCAGGATGCCAGATCATCAATTACCGTACAGATGCAAAGCAGAAAT GGTTGCTTCTCACTGGCATATCTGCACAGCAAAATCGTGTGGTTGGAGCTATGCAGCTGTATTCTGTAGA TAGGAAAGTTTCTCAGCCTATTGAAGGGCATGCAGCTAGTTTTGCACAATTTAAGATGGAAGGAAATGCA GAAGAATCAACATTATTTTGTTTTGCTGTGCGGGGTCAGGCAGGAGGAAAGTTACATATCATTGAAGTTG GTACCCCACCCACAGGGAATCAACCCTTCCCAAAGAAGGCAGTGGATGTTTTCTTTCCTCCAGAAGCACA AAATGACTTTCCTGTTGCAATGCAGATCAGTGAAAAGCATGATGTGGTGTTCTTGATTACCAAGTATGGT TATATTCACCTCTATGATCTTGAGACTGGCACGTGCATCTACATGAACAGAATCAGCGGAGAAACCATCT TCGTCACTGCACCTCATGAAGCCACAGCTGGAATAATAGGAGTCAACAGAAAGGGACAGGTCCTGTCAGT TTGTGTGGAAGAGGAGAACATAATTCCTTATATCACCAATGTCCTACAAAATCCTGACTTGGCTCTGAGA ATGGCTGTGCGGAACAACTTAGCTGGTGCTGAAGAACTCTTTGCCCGGAAATTTAATGCTCTCTTCGCCC AGGGAAACTACTCAGAGGCTGCAAAGGTGGCTGCTAATGCTCCAAAGGGAATCCTTCGTACTCCAGATAC CATCCGTCGATTCCAGAGTGTCCCAGCTCAGCCAGGTCAAACGTCACCTCTACTTCAGTATTTCGGAATC CTTTTGGACCAGGGCCAGCTGAACAAATACGAGTCCTTAGAGTTATGTAGACCTGTACTTCAGCAAGGGC GGAAACAGCTTTTGGAGAAATGGCTGAAAGAAGATAAGCTGGAATGTTCTGAAGAACTGGGTGATCTCGT TAAATCTGTGGACCCAACATTAGCACTGAGTGTTTACCTCAGGGCCAATGTACCTAATAAAGTCATCCAG TGCTTTGCAGAGACAGGCCAAGTCCAGAAGATTGTTTTATATGCTAAAAAAGTTGGCTATACTCCAGACT GGATCTTTCTCCTGAGAAATGTCATGCGGATCAGTCCAGATCAAGGACAGCAGTTTGCTCAAATGTTAGT TCAGGATGAAGAGCCTCTTGCTGATATCACCCAGATTGTGGATGTCTTTATGGAATACAATCTAATCCAA CAATGTACTGCCTTCTTACTTGATGCTTTGAAGAATAATCGTCCCTCTGAAGGTCCTTTGCAGACGCGAT TGCTTGAGATGAACCTTATGCACGCACCTCAAGTTGCAGATGCCATTCTCGGGAATCAGATGTTCACCCA CTATGACCGGGCTCATATCGCCCAGCTGTGTGAGAAGGCGGGCCTGCTGCAGCGTGCATTAGAACACTTC ACCGATTTATATGACATAAAACGTGCAGTTGTTCATACCCATCTTCTTAACCCTGAGTGGTTAGTGAATT ACTTTGGGTCCTTATCAGTTGAAGATTCCCTAGAATGCCTCAGAGCCATGCTGTCAGCTAATATTCGTCA AAATCTACAGATCTGTGTTCAGGTGGCTTCTAAATATCACGAACAGCTGTCAACTCAGTCTCTGATTGAG CTCTTTGAATCATTCAAGAGTTTTGAAGGTCTCTTTTATTTTCTGGGGTCCATTGTTAACTTTAGTCAGG ACCCAGATGTGCACTTTAAATATATTCAGGCGGCTTGCAAGACTGGGCAAATCAAAGAAGTGGAAAGAAT CTGCAGAGAAAGCAACTGCTATGATCCCGAGCGAGTCAAGAATTTTCTCAAGGAGGCAAAACTAACAGAT CAGTTGCCACTTATCATTGTCTGTGATCGGTTTGACTTTGTCCATGACCTGGTGCTGTATTTATATAGAA ACAATCTTCAGAAGTATATAGAGATATATGTCCAGAAGGTAAATCCAAGTCGACTTCCTGTGGTTATTGG AGGATTGCTGGATGTTGACTGCTCTGAAGATGTCATTAAAAATTTGATTCTTGTTGTGAGAGGTCAATTC TCTACTGATGAGCTTGTTGCTGAAGTCGAGAAAAGAAACAGATTGAAGCTGCTTCTTCCTTGGCTTGAGG CCAGAATTCATGAGGGCTGTGAGGAGCCTGCTACTCACAATGCACTTGCCAAAATATACATTGACAGTAA CAACAACCCAGAGAGATTTCTTCGTGAAAACCCTTATTATGACAGTCGTGTTGTTGGGAAGTACTGTGAG AAGAGAGACCCCCATCTGGCTTGTGTTGCTTATGAACGTGGCCAGTGTGACCTGGAGCTCATCAATGTTT GCAATGAGAATTCCCTCTTCAAAAGCCTTTCTCGCTACTTAGTCCGTCGAAAGGATCCAGAATTGTGGGG TAGTGTGCTACTGGAAAGCAATCCATACAGAAGACCGTTAATTGACCAGGTTGTACAGACAGCCTTGTCT GAAACTCAGGACCCAGAAGAAGTGTCAGTAACTGTGAAGGCTTTTATGACAGCAGATCTTCCTAATGAGC TCATTGAACTGCTGGAGAAAATCGTTCTTGATAACTCCGTATTCAGTGAGCACAGGAATCTGCAAAATCT CCTCATCCTCACTGCAATTAAGGCTGACCGAACACGTGTTATGGAGTATATTAACCGCCTGGATAATTAT GATGCTCCAGATATTGCCAACATTGCCATCAGCAATGAGCTGTTTGAAGAGGCATTTGCTATTTTCCGGA AGTTTGATGTCAATACTTCAGCAGTGCAGGTGTTGATTGAACATATTGGAAACCTGGATCGTGCATATGA GTTTGCTGAACGCTGCAATGAGCCTGCTGTTTGGAGTCAACTTGCAAAAGCTCAGTTGCAGAAAGGAATG GTGAAGGAAGCCATTGATTCTTACATCAAAGCAGATGATCCTTCATCTTACATGGAAGTTGTCCAGGCTG CCAATGCCAGCGGAAACTGGGAAGAATTGGTGAAGTATTTGCAGATGGCACGCAAGAAGGCTCGAGAGTC CTATGTGGAGACAGAATTGATATTTGCACTGGCTAAAACAAACCGCCTTGCAGAATTAGAAGAGTTCATC AATGGGCCAAATAATGCTCATATTCAGCAAGTTGGTGACCGTTGTTATGATGAAAAAATGTATGATGCTG CTAAGTTGTTGTACAATAATGTTTCTAACTTTGGACGCTTGGCATCTACCCTTGTTCACCTTGGCGAGTA TCAGGCAGCAGTTGATGGTGCCAGAAAAGCTAATAGTACTCGAACATGGAAAGAGGTCTGCTTTGCCTGT GTAGATGGGAAAGAGTTCCGCCTTGCTCAGATGTGTGGACTCCATATTGTGGTGCATGCAGATGAATTAG AGGAGCTTATCAACTACTATCAGGATCGTGGATATTTTGAAGAACTCATCACCATGTTGGAGGCAGCCTT GGGACTGGAGCGAGCTCACATGGGAATGTTCACAGAGCTAGCTATTTTGTATTCTAAATTCAAGCCACAG AAAATGAGAGAACACCTGGAGTTGTTCTGGTCGAGAGTGAATATTCCTAAGGTGCTGAGAGCTGCAGAAC AGGCTCATCTCTGGGCAGAGCTGGTGTTTCTGTATGACAAGTACGAAGAGTATGACAATGCCATCATTAC CATGATGAATCACCCCACCGATGCATGGAAGGAAGGGCAGTTCAAAGACATCATCACCAAGGTGGCTAAT GTGGAACTGTACTACAAAGCAATCCAGTTCTACTTAGAATTCAAGCCTTTGTTGTTAAATGACTTGCTCA TGGTGCTGTCTCCACGGTTGGATCACACTCGTGCAGTCAATTATTTCAGCAAGGTAAAACAGCTACCACT GGTGAAACCATATTTACGCTCAGTGCAGAACCACAACAACAAATCTGTGAATGAATCACTGAACAACCTC TTTATTACTGAAGAAGATTACCAGGCTCTGCGAACATCAATAGATGCTTATGACAACTTTGACAACATCT CACTTGCTCAGCGTTTGGAAAAACACGAACTCATTGAATTCAGAAGAATTGCTGCTTATCTCTTCAAAGG CAACAATCGCTGGAAACAGAGTGTAGAGTTGTGCAAGAAAGACAGCCTTTACAAGGATGCAATGCAGTAT GCCTCTGAATCTAAAGACACAGAATTGGCTGAAGAGCTCCTACAGTGGTTTTTGCAGGAAGAAAAAAGAG AATGCTTTGGAGCTTGTCTCTTTACCTGTTATGATCTCTTAAGGCCAGATGTCGTCCTGGAAACCGCATG GAGACATAATATCATGGATTTCGCCATGCCCTATTTCATCCAGGTCATGAAGGAATATTTGACCAAGGTG GATAAATTAGATGCTTCGGAGTCGCTGAGAAAAGAAGAAGAACAAGCTACAGAGACACAGCCCATTGTTT ATGGTCAGCCCCAGCTGATGCTAACAGCAGGACCCAGTGTGGCAGTCCCTCCCCAGGCACCTTTTGGTTA TGGTTATACTGCACCACCGTATGGGCAGCCACAGCCTGGCTTTGGGTACAGCATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG212064 representing NM_001003908
Red=Cloning site Green=Tags(s) MAQILPIRFQEHLQLQNLGINPANIGFSTLTMESDKFICIREKVGEQAQVVIIDMNDPSNPIRRPISADS AIMNPASKVIALKAGKTLQIFNIEMKSKMKAHTMTDDVTFWKWISLNTVALVTDNAVYHWSMEGESQPVK MFDRHSSLAGCQIINYRTDAKQKWLLLTGISAQQNRVVGAMQLYSVDRKVSQPIEGHAASFAQFKMEGNA EESTLFCFAVRGQAGGKLHIIEVGTPPTGNQPFPKKAVDVFFPPEAQNDFPVAMQISEKHDVVFLITKYG YIHLYDLETGTCIYMNRISGETIFVTAPHEATAGIIGVNRKGQVLSVCVEEENIIPYITNVLQNPDLALR MAVRNNLAGAEELFARKFNALFAQGNYSEAAKVAANAPKGILRTPDTIRRFQSVPAQPGQTSPLLQYFGI LLDQGQLNKYESLELCRPVLQQGRKQLLEKWLKEDKLECSEELGDLVKSVDPTLALSVYLRANVPNKVIQ CFAETGQVQKIVLYAKKVGYTPDWIFLLRNVMRISPDQGQQFAQMLVQDEEPLADITQIVDVFMEYNLIQ QCTAFLLDALKNNRPSEGPLQTRLLEMNLMHAPQVADAILGNQMFTHYDRAHIAQLCEKAGLLQRALEHF TDLYDIKRAVVHTHLLNPEWLVNYFGSLSVEDSLECLRAMLSANIRQNLQICVQVASKYHEQLSTQSLIE LFESFKSFEGLFYFLGSIVNFSQDPDVHFKYIQAACKTGQIKEVERICRESNCYDPERVKNFLKEAKLTD QLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVIGGLLDVDCSEDVIKNLILVVRGQF STDELVAEVEKRNRLKLLLPWLEARIHEGCEEPATHNALAKIYIDSNNNPERFLRENPYYDSRVVGKYCE KRDPHLACVAYERGQCDLELINVCNENSLFKSLSRYLVRRKDPELWGSVLLESNPYRRPLIDQVVQTALS ETQDPEEVSVTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLILTAIKADRTRVMEYINRLDNY DAPDIANIAISNELFEEAFAIFRKFDVNTSAVQVLIEHIGNLDRAYEFAERCNEPAVWSQLAKAQLQKGM VKEAIDSYIKADDPSSYMEVVQAANASGNWEELVKYLQMARKKARESYVETELIFALAKTNRLAELEEFI NGPNNAHIQQVGDRCYDEKMYDAAKLLYNNVSNFGRLASTLVHLGEYQAAVDGARKANSTRTWKEVCFAC VDGKEFRLAQMCGLHIVVHADELEELINYYQDRGYFEELITMLEAALGLERAHMGMFTELAILYSKFKPQ KMREHLELFWSRVNIPKVLRAAEQAHLWAELVFLYDKYEEYDNAIITMMNHPTDAWKEGQFKDIITKVAN VELYYKAIQFYLEFKPLLLNDLLMVLSPRLDHTRAVNYFSKVKQLPLVKPYLRSVQNHNNKSVNESLNNL FITEEDYQALRTSIDAYDNFDNISLAQRLEKHELIEFRRIAAYLFKGNNRWKQSVELCKKDSLYKDAMQY ASESKDTELAEELLQWFLQEEKRECFGACLFTCYDLLRPDVVLETAWRHNIMDFAMPYFIQVMKEYLTKV DKLDASESLRKEEEQATETQPIVYGQPQLMLTAGPSVAVPPQAPFGYGYTAPPYGQPQPGFGYSM TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001003908 |
| ORF Size | 5025 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001003908.2 |
| RefSeq Size | 6178 bp |
| RefSeq ORF | 5028 bp |
| Locus ID | 67300 |
| UniProt ID | Q68FD5 |
| Gene Summary | Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension. Plays a role in early autophagosome formation.[UniProtKB/Swiss-Prot Function] |
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