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Pde3b (NM_011055) Mouse Tagged ORF Clone
CAT＃: MG219273
- TrueORF®
Pde3b (tGFP-tagged) - Mouse phosphodiesterase 3B cGMP-inhibited (Pde3b), (10ug)
ORF Plasmid: DDK
"NM_011055" in other vectors (4)
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CNY 10,550.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 9830102A01Rik; AI847709 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG219273 representing NM_011055
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGAAAGACGAGCGCGAGCGGGACGCGCCAGCCATGAGGTCCCCGCCGCCGCCGCCAGCCTCGGCCG CCTCGCCCCCCGAGAGCCTGCGCAACGGCTACGTGAAGAGCTGCGTGAGCCCGCTGCGGCAGGACCCTCC GCGCAGCTTCTTCTTCCACCTCTGCCGCTTCTGCAACGTGGAGCCGCCGGCGGCCTCGCTCCGCGCCGGG GCACGCCTCTCGCTCGGCGTCCTGGCCGCCTTTGTCCTGGCCGCGCTGCTGGGCGCGAGGCCCGAGCGCT GGGCGGCCGCGGCAGCCGGGCTCCGGACGCTGCTGAGCGCCTGCTCGCTCAGCCTCAGCCCGCTCTTCAG CATCGCCTGTGCCTTCTTCTTCCTCACCTGTTTCCTGACGCGCGCGCAGCGCGGCCCGGGCCGCGGCGCC GGCTCCTGGTGGCTGCTGGCGCTGCCCGCCTGCTGCTACCTGGGCGACTTCGCGGCGTGGCAGTGGTGGT CGTGGCTGCGTGGGGAGCCGGCGGCGGCGGGCCGGCTCTGCCTGGTGCTGAGCTGCGTGGGGCTGCTGAC GCTCGCGCCCCGCGTGAGGCTGCGGCACGGCGTCCTGGTGCTGCTCTTCGCCGGCCTGGTGTGGTGGGTG TCCTTCTCCGGCCTCGGGGCTCTGCCGCCCGCGCTCAGGCCTCTGCTGTCGTGCCTGGTCGGGGGCGCGG GATGCCTGCTAGCCCTGGGCTTGGACCACTTCTTTCACGTCCGGGGAGCCTCCCCTCCGCCGCGCTCGGC GAGTACCGCGGAGGAAAAAGTGCCTGTGATCAGACCCCGGAGGAGGTCCAGCTGCGTGTCGCTGGGAGAA AGCGCAGCCGGTTACTATGGCAGTGGCAAGATGTTCAGGAGACCGTCGTTGCCTTGTATTTCCCGAGAAC AGATGATCCTCTGGGACTGGGACTTGAAGCAGTGGTGTAAACCTCATTACCAGAATTCTGGAGGTGGAAA TGGAGTTGATCTTTCAGTGCTAAATGAAGCTCGCAATATGGTGTCAGACCTGCTGATTGACCCAAGCCTT CCCCCACAAGTCATTTCTTCTCTGCGGAGTATCAGTAGCTTGATGGGTGCTTTCTCAGGTTCCTGTAGGC CAAAGATTAATTCTTTTACACCATTTCCTGGATTTTATCCCTGCTCTGAAGTAGAAGATCCAGTTGAGAA AGGAGATCGAAAACTTCACAAGGGATTGAGTGGCAGAACCAGTTTCCCAACTCCACAGCTGAGGAGGAGC TCTGGAGCCTCATCGTTGCTGACTAATGAGCACTGTTCAAGGTGGGATCGCAGCAGTGGTAAGAGGTCTT ACCAAGAACTCAGTGTTTCAAGTCATGGATGCCACCTAAATGGGCCTTTTAGTTCAAATCTTTTTACAAT TCCAAAGCAGAGGTCATCGTCTGTGTCACTGACGCACCATGCAGGTCTGAGAAGAGCTGGTGCTTTGCCC AGCCACAGTCTTCTGAATTCTTCAAGCCATGTACCAGTGTCTGCTGGCTCTCTAACTAATCGATCACCCA TAGGATTTCCTGATACCACTGATTTTCTTACTAAGCCAAATATTATTTTACATAGATCACTGGGCAGTGT ATCAAGTGCAGCAGATTTCCATCAGTACCTTAGGAACTCTGACAGCAATCTGTGTAGCAGCTGTGGACAC CAAATACTCAAATATGTTTCAACATGTGAACCCGATGGTACAGACCACCCCAGTGAAAAATCAGGTGAAG AAGACAGCAGTGTTTTCTCAAAAGAACCATTGAACATTGTGGAAACCCAAGAAGAAGAGACCATGAAGAA AGCCTGCAGGGAGTTATTTTTGGAAGGTGATAGTCACCTGATGGAAGAGGCACAGCAACCAAATATCGAT CAGGAAGTGTCACTGGATCCAATGTTAGTAGAAGATTATGATTCATTAATAGAAAAGATGAACAACTGGA ATTTTCAGATTTTTGAGCTTGTAGAAAAGATGGGAGAAAAATCAGGAAGGATTCTCAGTCAGGTTATGTA TACTTTATTTCAAGATACTGGTTTATTGGAAACATTTAAAATTCCCACTCAAGAATTTATGAATTATTTT CGTGCCTTAGAAAATGGCTACCGGGACATTCCATATCACAATCGTGTGCATGCCACAGATGTCCTACATG CTGTTTGGTATTTGACAACCCGACCAATTCCTGGCTTACCTCAGATCCATAATAACCATGAAACGGAAAC CAAAGCAGATTCAGATGGTAGACTTGGTTCTGGACAGATTGCTTACATTTCTTCGAAGAGTTGCTGTATT CCAGATATGAGTTATGGCTGCCTATCTTCAAACATCCCTGCATTAGAATTGATGGCTTTGTATGTGGCAG CTGCCATGCACGATTACGATCACCCAGGAAGAACAAATGCGTTTCTAGTGGCTACAAATGCACCTCAGGC AGTTTTATACAATGACAGATCTGTTCTAGAGAATCATCATGCTGCATCAGCTTGGAATCTGTATCTTTCT CGCCCAGAGTACAACTTCCTCCTTAACCTTGATCACATGGAATTCAAGCGGTTTCGATTTTTAGTTATAG AAGCAATCCTTGCTACAGATCTCAAAAAACATTTTGATTTCCTTGCAGAATTCAATGCCAAGGCCAATGA TGTAAATAGTAACGGTATAGAATGGAGCAGTGAAAACGATCGCCTCTTGGTCTGCCAGGTGTGCATCAAA TTAGCAGATATCAATGGCCCAGCAAAAGATCGGGACCTACATTTGAGATGGACAGAAGGCATTGTGAATG AATTTTATGAGCAGGGAGATGAAGAAGCAACCCTGGGTCTACCTATTAGTCCCTTCATGGATCGTTCTTC TCCTCAACTAGCAAAACTCCAAGAATCTTTTATCACCCACATTGTGGGCCCCCTGTGCAACTCCTATGAT GCTGCTGGCTTGCTGCCGGGTCAGTGGATAGAAACAGAAGAGGGTGATGATACAGAAAGTGATGATGATG ATGATGATGATGATGGTGGTGGTGAAGAATTAGATTCAGATGATGAAGAAACAGAAGACAATCTAAATCC TAAACCACAAAGAAGGAAAGGCAGGCGGCGAATATTTTGCCAACTAATGCACCACCTCACTGAAAACCAC AAGATATGGAAGGAAATCATAGAAGAAGAAGAAGAAAAATGTAAAGCTGAGGGGAACAAACTGCAGGTGG ATAATGCCTCCTTACCTCAGGCAGATGAGATTCAGGTTATTGAAGAAGCAGATGAAGAGGAAGAACAAAT GTTTGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG219273 representing NM_011055
Red=Cloning site Green=Tags(s) MRKDERERDAPAMRSPPPPPASAASPPESLRNGYVKSCVSPLRQDPPRSFFFHLCRFCNVEPPAASLRAG ARLSLGVLAAFVLAALLGARPERWAAAAAGLRTLLSACSLSLSPLFSIACAFFFLTCFLTRAQRGPGRGA GSWWLLALPACCYLGDFAAWQWWSWLRGEPAAAGRLCLVLSCVGLLTLAPRVRLRHGVLVLLFAGLVWWV SFSGLGALPPALRPLLSCLVGGAGCLLALGLDHFFHVRGASPPPRSASTAEEKVPVIRPRRRSSCVSLGE SAAGYYGSGKMFRRPSLPCISREQMILWDWDLKQWCKPHYQNSGGGNGVDLSVLNEARNMVSDLLIDPSL PPQVISSLRSISSLMGAFSGSCRPKINSFTPFPGFYPCSEVEDPVEKGDRKLHKGLSGRTSFPTPQLRRS SGASSLLTNEHCSRWDRSSGKRSYQELSVSSHGCHLNGPFSSNLFTIPKQRSSSVSLTHHAGLRRAGALP SHSLLNSSSHVPVSAGSLTNRSPIGFPDTTDFLTKPNIILHRSLGSVSSAADFHQYLRNSDSNLCSSCGH QILKYVSTCEPDGTDHPSEKSGEEDSSVFSKEPLNIVETQEEETMKKACRELFLEGDSHLMEEAQQPNID QEVSLDPMLVEDYDSLIEKMNNWNFQIFELVEKMGEKSGRILSQVMYTLFQDTGLLETFKIPTQEFMNYF RALENGYRDIPYHNRVHATDVLHAVWYLTTRPIPGLPQIHNNHETETKADSDGRLGSGQIAYISSKSCCI PDMSYGCLSSNIPALELMALYVAAAMHDYDHPGRTNAFLVATNAPQAVLYNDRSVLENHHAASAWNLYLS RPEYNFLLNLDHMEFKRFRFLVIEAILATDLKKHFDFLAEFNAKANDVNSNGIEWSSENDRLLVCQVCIK LADINGPAKDRDLHLRWTEGIVNEFYEQGDEEATLGLPISPFMDRSSPQLAKLQESFITHIVGPLCNSYD AAGLLPGQWIETEEGDDTESDDDDDDDDGGGEELDSDDEETEDNLNPKPQRRKGRRRIFCQLMHHLTENH KIWKEIIEEEEEKCKAEGNKLQVDNASLPQADEIQVIEEADEEEEQMFE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011055 |
| ORF Size | 3297 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011055.2, NP_035185.2 |
| RefSeq Size | 5314 bp |
| RefSeq ORF | 3300 bp |
| Locus ID | 18576 |
| UniProt ID | Q61409 |
| Gene Summary | Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. May play a role in fat metabolism. Regulates cAMP binding of RAPGEF3. Through simultaneous binding to RAPGEF3 and PIK3R6 assembles a signaling complex in which the PI3K gamma complex is activated by RAPGEF3 and which is involved in angiogenesis (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223550 | Pde3b (untagged) - Mouse phosphodiesterase 3B, cGMP-inhibited (Pde3b), (10ug) |
CNY 14,060.00 |
|
| MR219273 | Pde3b (Myc-DDK-tagged) - Mouse phosphodiesterase 3B, cGMP-inhibited (Pde3b) |
CNY 7,656.00 |
|
| MR219273L3 | Lenti ORF clone of Pde3b (Myc-DDK-tagged) - Mouse phosphodiesterase 3B, cGMP-inhibited (Pde3b) |
CNY 11,500.00 |
|
| MR219273L4 | Lenti ORF clone of Pde3b (mGFP-tagged) - Mouse phosphodiesterase 3B, cGMP-inhibited (Pde3b) |
CNY 11,500.00 |
