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Arfgap1 (NM_001177708) Mouse Tagged ORF Clone
CAT＃: MG220367
- TrueORF®
Arfgap1 (tGFP-tagged) - Mouse ADP-ribosylation factor GTPase activating protein 1 (Arfgap1) transcript variant 5, (10ug)
ORF Plasmid: DDK
"NM_001177708" in other vectors (4)
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CNY 5,440.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI115377; Arf1gap |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG220367 representing NM_001177708
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGCCCAAGAACCAGGAAAGTTCTTAAGGAAGTCCGGGCACAGGATGAGAATAATGTTTGCTTTG AGTGTGGTGCGTTCAATCCTCAGTGGGTCAGCGTGACTTATGGCATCTGGATCTGCCTGGAATGCTCTGG GAGACACCGTGGGCTTGGGGTGCACCTCAGCTTTGTGCGCTCTGTTACAATGGACAAATGGAAGGACATT GAGCTGGAGAAGATGAAGGCTGGTGGGAATGCTAAGTTCCGAGAGTTCCTGGAGACACAGGACGACTATG AGCCTAGCTGGTCACTGCAGGACAAGTACAGCAGCAGAGCCGCGGCGCTCTTCAGGGATAAGGTGGCTAC TTTGGCAGAAGGTAAAGAGTGGTCTCTGGAGTCATCGCCTGCACAGAACTGGACCCCACCTCAGCCCAAG ACACTGCAGTTCACTGCCCACCGAGCCTCTGGCCAGCCACAGAGTGCAGCCGCCTCTGGGGACAAGGCTT TTGAAGATTGGCTGAATGATGACCTGGGTTCCTACCAGGGTGCTCAGGAGAATCGCTATGTAGGGTTTGG GAACACAGTGCCACCTCAGAAGAGAGAAGATGACTTCCTCAACAATGCCATGTCATCGCTGTACTCGGGC TGGAGCAGTTTTACCACTGGGGCGAGCAAGTTTGCATCTGCAGCAAAGGAGGGTGCTACAAAATTTGGAT CTCAAGCAAGTCAGAAGGCTTCGGAGTTGGGCCACAGCCCTGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG220367 representing NM_001177708
Red=Cloning site Green=Tags(s) MASPRTRKVLKEVRAQDENNVCFECGAFNPQWVSVTYGIWICLECSGRHRGLGVHLSFVRSVTMDKWKDI ELEKMKAGGNAKFREFLETQDDYEPSWSLQDKYSSRAAALFRDKVATLAEGKEWSLESSPAQNWTPPQPK TLQFTAHRASGQPQSAAASGDKAFEDWLNDDLGSYQGAQENRYVGFGNTVPPQKREDDFLNNAMSSLYSG WSSFTTGASKFASAAKEGATKFGSQASQKASELGHSPE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001177708 |
| ORF Size | 1182 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001177708.1, NP_001171179.1 |
| RefSeq Size | 2448 bp |
| RefSeq ORF | 1185 bp |
| Locus ID | 228998 |
| UniProt ID | Q9EPJ9 |
| Gene Summary | GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC212846 | Arfgap1 (untagged) - Mouse ADP-ribosylation factor GTPase activating protein 1 (Arfgap1), transcript variant 5, (10ug) |
CNY 3,990.00 |
|
| MR220367 | Arfgap1 (Myc-DDK-tagged) - Mouse ADP-ribosylation factor GTPase activating protein 1 (Arfgap1), transcript variant 5 |
CNY 2,470.00 |
|
| MR220367L3 | Lenti ORF clone of Arfgap1 (Myc-DDK-tagged) - Mouse ADP-ribosylation factor GTPase activating protein 1 (Arfgap1), transcript variant 5 |
CNY 4,370.00 |
|
| MR220367L4 | Lenti ORF clone of Arfgap1 (mGFP-tagged) - Mouse ADP-ribosylation factor GTPase activating protein 1 (Arfgap1), transcript variant 5 |
CNY 4,370.00 |
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