Cnot2 (NM_028082) Mouse Tagged ORF Clone
CAT#: MG223703
- TrueORF®
Cnot2 (tGFP-tagged) - Mouse CCR4-NOT transcription complex subunit 2 (Cnot2) transcript variant 3, (10ug)
ORF Plasmid: DDK
"NM_028082" in other vectors (4)
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CNY 5,420.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 2600016M12Rik; 2810470K03Rik; AA537049; AA959607; AW557563; C79650 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG223703 representing NM_028082
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGAGGACTGATGGACATACATTATCTGAGAAAAGAAACTACCAGATGCTGGCATCGCCATCTACGT CAGGTCAGCTGTCTCAATTTGGGGCAAGTTTATACGGGCAACAAAGTGCACTAGGCCTTCCAATGAGGGG GATGAGCAACAATACCCCTCAGTTAAATCGCAGCTTATCACAAGGCACTCAGTTACCGAGCCACGTCACG CCAACAACAGGGGTACCAACAATGTCACTTCACACGCCTCCATCTCCAAGCAGGGGTATTTTGCCTATGA ATCCTAGGAATATGATGAACCACTCCCAGGTTGGTCAGGGCATTGTAATTCCTAGCAGGACAAATAGCAT GAGCAGTTCAGGGTTAGGTAGCCCCAACAGAAGCTCGCCAAGCATAATATGTATGCCAAAGCAACAGCCT TCTCGACAGCCTTTTACTGTGAACAGTATGTCTGGATTTGGAATGAACAGGAATCAGGCATTTGGAATGA ATAACTCCTTATCAAGTAACATTTTTAATGGAACAGATGGCAGTGAAAATGTGACAGGATTGGACCTTTC AGATTTCCCAGCGTTAGCTGACCGGAATAGAAGGGAAGGGAGCGGCAACCCAACTCCATTAATAAACCCC TTGGCTGGAAGAGCTCCTTACGTTGGAATGGTAACAAAACCTGCAAATGAGCAATCCCAAGACTTCTCAA TACACAACGAAGATTTTCCAGCATTACCTGGTTCCAGCTATAAAGATCCAACGTCAAGTAATGACGACAG CAAATCTAATTTGAGTACATCGGGGAAGACGACTTCAAGTACAGATGGACCCAAATTCCCTGGAGATAAA AGTTCAACAACACAAAACAATAATCAACAGAAAAAGGGGATCCAGGTGTTACCTGATGGTCGAGTTACTA ACATTCCTCAAGGGATGGTGACGGACCAATTTGGAATGATTGGCCTGTTAACATTTATCAGGGCAGCAGA GACAGACCCAGGAATGGTACATCTTGCTTTAGGAAGTGACTTAACAACATTAGGCCTCAATCTGAACTCA CCTGAAAATCTCTATCCCAAATTTGCATCACCCTGGGCTTCTTCACCTTGTCGGCCTCAAGACATAGACT TCCATGTTCCATCAGAATATTTAACAAACATTCACATTAGGGATAAGCTGGCTGCAATCAAACTTGGCCG ATATGGAGAAGACCTCCTCTTCTATCTGTATTACATGAACGGCGGGGATGTATTACAACTCTTAGCTGCG GTAGAACTTTTTAACCGTGATTGGAGATACCACAAGGAGGAGCGGGTATGGATTACCAGGGCACCAGGCA TGGAGCCAACAATGAAAACCAACACGTATGAGCGGGGGACCTACTATTTCTTTGACTGTCTCAACTGGAG GAAAGTAGCTAAGGAGTTCCATCTGGAATATGACAAATTAGAAGAGCGGCCTCACCTGCCATCCACCTTC AACTACAACCCTGCTCAGCAAGCCTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG223703 representing NM_028082
Red=Cloning site Green=Tags(s) MVRTDGHTLSEKRNYQMLASPSTSGQLSQFGASLYGQQSALGLPMRGMSNNTPQLNRSLSQGTQLPSHVT PTTGVPTMSLHTPPSPSRGILPMNPRNMMNHSQVGQGIVIPSRTNSMSSSGLGSPNRSSPSIICMPKQQP SRQPFTVNSMSGFGMNRNQAFGMNNSLSSNIFNGTDGSENVTGLDLSDFPALADRNRREGSGNPTPLINP LAGRAPYVGMVTKPANEQSQDFSIHNEDFPALPGSSYKDPTSSNDDSKSNLSTSGKTTSSTDGPKFPGDK SSTTQNNNQQKKGIQVLPDGRVTNIPQGMVTDQFGMIGLLTFIRAAETDPGMVHLALGSDLTTLGLNLNS PENLYPKFASPWASSPCRPQDIDFHVPSEYLTNIHIRDKLAAIKLGRYGEDLLFYLYYMNGGDVLQLLAA VELFNRDWRYHKEERVWITRAPGMEPTMKTNTYERGTYYFFDCLNWRKVAKEFHLEYDKLEERPHLPSTF NYNPAQQAF TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_028082 |
ORF Size | 1497 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_028082.2, NP_082358.2 |
RefSeq Size | 2693 bp |
RefSeq ORF | 1500 bp |
Locus ID | 72068 |
UniProt ID | Q8C5L3 |
Gene Summary | Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Required for the CCR4-NOT complex structural integrity. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may specifically involve the N-Cor repressor complex containing HDAC3, NCOR1 and NCOR2. Involved in the maintenance of embryonic stem (ES) cell identity; prevents their differentiation towards extraembryonic trophectoderm lineages.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC217017 | Cnot2 (untagged) - Mouse CCR4-NOT transcription complex, subunit 2 (Cnot2), transcript variant 3, (10ug) |
CNY 6,460.00 |
|
MR223703 | Cnot2 (Myc-DDK-tagged) - Mouse CCR4-NOT transcription complex, subunit 2 (Cnot2), transcript variant 3 |
CNY 4,940.00 |
|
MR223703L3 | Lenti ORF clone of Cnot2 (Myc-DDK-tagged) - Mouse CCR4-NOT transcription complex, subunit 2 (Cnot2), transcript variant 3 |
CNY 6,840.00 |
|
MR223703L4 | Lenti ORF clone of Cnot2 (mGFP-tagged) - Mouse CCR4-NOT transcription complex, subunit 2 (Cnot2), transcript variant 3 |
CNY 6,840.00 |