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Zfp451 (NM_133817) Mouse Tagged ORF Clone
CAT＃: MG224936
- TrueORF®
Zfp451 (tGFP-tagged) - Mouse zinc finger protein 451 (Zfp451), (10ug)
ORF Plasmid: DDK
"NM_133817" in other vectors (4)
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CNY 11,590.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 4930515K21Rik; 4933435G09Rik; AI596398; COASTER; Kiaa0576-hp; mKIAA1702; Znf451 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG224936 representing NM_133817
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGACCCAGGGCCGGAGATAATAGAATCTGTCCCACCAGCTGGGCCCGAGGCATCAGAGTCTACCA CGGATGAAAACGAAGATGACATTCAGTTTGTTAGTGAAGGACCGTTAAGACCCGTTTTGGAATACATTGA TCTAGTCAGCAGTGATGATGAAGAGCCTAGTACCTCTCACAGTGATGAGAATTTTAAATGCAAAGACTAT ATAGATCACCAGAAGGATAAAGTTGCTTTAACTTTGGCTCGCCTGGCCCGCCATGTTGAAGTAGAAAAAC AACAGAAAGAAGAGAAGAACAGGGCATTTAGGGAAAAAATTGATTTTCAGCACGCTCATGGTTTACAAGA GTTGGAGTTTATTCAAGGACATTCAGAAACAGAAGCAGCAAGACAGTGTGTGGACCAGTGGCTAAAAATG CCAGGACTCAGAACAAATGCAGCTAATTCTGGAACAAAAAGATCATTCCAACGTGGAGGCAGGATGTGGA GGTCTGAGAAGCCAATTTTGTGTCCTATAATGCACTGTAACAAGGAATTTGACAATGGGCACCTTCTGTT GGGACATTTGAAAAGGTTTGATCACTCCCCATGTGATCCCACAATTACGTTACATGGACCTCTGGCTAAT TCGTTTGCATGTGCAGTGTGCTATGAACATTTCGTTACTCAACAGCAGTACAAGGACCATCTTCTCTCCA GGACAGCTGCAGCCGATGGACATAGCAATAGCCTTCTTCCTCAGATTATTCAATGTTATGCTTGTCCACA GTGCTTCCTTTTGTTTAGCACCAAGGATGAGTGTTTGAAGCATATGTCTACAAAGAATCATTTCCATCAG AGCTTTAAACTGAGTGATAATAAAGGAACGGCCCGCCCAATATCATTTCCATCTTTTGCAAAGAAGCGTT TGGTCTCTCTGTGCAAAGATGTTCCATTTCAGGTTAAGTGTGTGGCCTGCCACCAGACTCTGCGTTCTCA CATGGAGCTCACTGCCCATTTCAGGGTTCGTTGTCAAAATGCTGGACCTGTTGCTATAGCTGAAAAAAGC ATTACTCAGGTTGCAAAAGAATTCATAGTAAGAGGTTATTGTTCAGATTGCAACCAGGTCTTTATGGATG TAGCCAGCACCCAAAGTCACAAGAATTCAGGACACAAAATTACACTTGCGAACTCGGTGGAAGAATCTGT CTTGCTTTATTGCCACATCAGTGAAGGGAGTCGGCCTCCTTGTGATTTACATTTATTTAGTCAACCAAAA ATTTCATCACTTAAACGGATTCTGTCCGTTAAAGAGTCCAGTGCAGAGGATTGTATCGTTCCGACAAAGA AGGTGAACTTAGGTGTTGAAAGCCTTGGAGGTGCAACTCGTGTGCAGAGGCAGAGTCCAGCAGTCACAGC CTGGTTTTGTGAATGCAGACGGCAGTTTCCCAGTGAAGAGGCGGTAGAAAAGCATGTTTTCTCGGCAAAC ACAATGTGTTATAAGTGTGTGGTCTGTGGAAAGGTTTGTGAAGATTCGGGGGTCATGCGTTTACACATGA GCCGGTTTCATGGAGGGGCGCATTTAAATAACTTTCTATTTTGGTGTCGGACGTGCAAAAAGGAGTTAGT AAAGAAAGATGCCATCATGGCACACATTACTGAGTTTCATAGTGGGCATAGATATTTTTATGAGATGGAT GAGGTAGAGGAGGAAGAGGAGGAGGCCATGCCGTCATCCTCTGTGGAGAGCCATTTGAATACTGACAAAC CTCCTTCACCCATTGCTGTTGTTGATCACTGCCCGGCCAACAGTCCTCCCAGGGGCAGGTGGCAGTGCCG CATCTGTGAGGACATGTTTGAATCCCAGGAGTGTGTGAAGCAGCACTGCATGTCCTTGACAAGCCACCGG TTTCACAGATACAGCTGTGCCCACTGCAGAAAGACTTTCCATAAAGTGGAAACTCTGTACCGACATTGCC AGGACGAGCATGACAGTGAGATCATGATGAAGTACTTCTGTGGGCTTTGTGATCTCATTTTTAACAAGGA AGAAGAATTTCTGAGTCACTATAAGGAACACCACAGCATAGATTATGTGTTTGTGTCAGAAAAAACTAAA ACCTCAATTAAAACTGAAGGCGATTTTAAAATAGTAGAGACCAGTAGTTTACTAAGCTGTGGCTGTCATG AGAGTTACATGTGTAAAATCAACAGAAAGGAAGACTATGACCGATGCCTTCCAGTCCTGCTGGAGAAAGG TAGGCTGTGGTTTCGCTGCAGCTCGTGCTCAGCCACAGCACAGAATGTGACCGACATTAACACTCACGTC TGCCAAGTGCACAGAAAAGAGAAAAGTGAGGAGGAGCAGCAGTATGTGATCAAGTGTGGCATCTGCACCA AGGCATTCCAGAACACGGAGAGCGCTCAGCAGCACTTCCACAGGAAGCACGCGGCCCTCCAGAAACCCAC CGCGACCCCAGGGGGAGCCAACAGAAGCAGCACATGCCAGCTGGCTGCTAGTGCCTCACATGCTGAGAAA AACCTGAAACAGCCTAGCTCTCAGAAACATTCAGACGTGGAAAAAGGAGCTGAGCATGATGTACGCTGCC AGAACATAGAGGAGGAAGTTGAGCTTCCGGATGTGGACTACCTGCGGACCATGACTCACATAGTCTTTGT AGATTTTGATAACTGGTCCAACTTTTTTGGTCATCTACCTGGGCATCTTAATCAAGGAACGTTTATTTGG GGCTTTCAAGGGGGAAACACCAACTGGAAGCCCCCGCTCAGCTGTAAGGTCTATAATTACTTGAGCAGGA TTGGCTGCTTCTTCCTTCATCCTCGCTGCAGTAAAAGAAAAGATGCCGCGGATTTTGCCATATGTATGCA TGCTGGCCGTCTAGATGAGCAACTTCCCAAACAAATTCCTTTCACCATCCTCTCGGGAGACCAAGGCTTT CTGGAGCTAGAGAATCAATTTAAGAAGACCCAGAGGCCAGCTCATATACTAAACCCTCACCACTTAGAGG GAGACATGATGTGTGCCTTGTTAAATAGCATATCTGATACTACCAAAGAGTGTGACAGTGACGATAGCTC GGGGATGAAAGGATCTCCAGCAGAGGAGCTCAGGGCCACGGAGGATGTGGAATTAGAAGAAGCTATTAGA AGAAGTCTTGAGGAGATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG224936 representing NM_133817
Red=Cloning site Green=Tags(s) MGDPGPEIIESVPPAGPEASESTTDENEDDIQFVSEGPLRPVLEYIDLVSSDDEEPSTSHSDENFKCKDY IDHQKDKVALTLARLARHVEVEKQQKEEKNRAFREKIDFQHAHGLQELEFIQGHSETEAARQCVDQWLKM PGLRTNAANSGTKRSFQRGGRMWRSEKPILCPIMHCNKEFDNGHLLLGHLKRFDHSPCDPTITLHGPLAN SFACAVCYEHFVTQQQYKDHLLSRTAAADGHSNSLLPQIIQCYACPQCFLLFSTKDECLKHMSTKNHFHQ SFKLSDNKGTARPISFPSFAKKRLVSLCKDVPFQVKCVACHQTLRSHMELTAHFRVRCQNAGPVAIAEKS ITQVAKEFIVRGYCSDCNQVFMDVASTQSHKNSGHKITLANSVEESVLLYCHISEGSRPPCDLHLFSQPK ISSLKRILSVKESSAEDCIVPTKKVNLGVESLGGATRVQRQSPAVTAWFCECRRQFPSEEAVEKHVFSAN TMCYKCVVCGKVCEDSGVMRLHMSRFHGGAHLNNFLFWCRTCKKELVKKDAIMAHITEFHSGHRYFYEMD EVEEEEEEAMPSSSVESHLNTDKPPSPIAVVDHCPANSPPRGRWQCRICEDMFESQECVKQHCMSLTSHR FHRYSCAHCRKTFHKVETLYRHCQDEHDSEIMMKYFCGLCDLIFNKEEEFLSHYKEHHSIDYVFVSEKTK TSIKTEGDFKIVETSSLLSCGCHESYMCKINRKEDYDRCLPVLLEKGRLWFRCSSCSATAQNVTDINTHV CQVHRKEKSEEEQQYVIKCGICTKAFQNTESAQQHFHRKHAALQKPTATPGGANRSSTCQLAASASHAEK NLKQPSSQKHSDVEKGAEHDVRCQNIEEEVELPDVDYLRTMTHIVFVDFDNWSNFFGHLPGHLNQGTFIW GFQGGNTNWKPPLSCKVYNYLSRIGCFFLHPRCSKRKDAADFAICMHAGRLDEQLPKQIPFTILSGDQGF LELENQFKKTQRPAHILNPHHLEGDMMCALLNSISDTTKECDSDDSSGMKGSPAEELRATEDVELEEAIR RSLEEM TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133817 |
| ORF Size | 3168 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133817.3, NP_598578.1 |
| RefSeq Size | 3650 bp |
| RefSeq ORF | 3171 bp |
| Locus ID | 98403 |
| UniProt ID | Q8C0P7 |
| Gene Summary | E3 SUMO-protein ligase; has a preference for SUMO2 and SUMO3 and facilitates UBE2I/UBC9-mediated sumoylation of target proteins. Plays a role in protein SUMO2 modification in response to stress caused by DNA damage and by proteasome inhibitors (in vitro). Required for MCM4 sumoylation. Has no activity with SUMO1 (PubMed:26524493). Preferentially transfers an additional SUMO2 chain onto the SUMO2 consensus site 'Lys-11'. Negatively regulates transcriptional activation mediated by the SMAD4 complex in response to TGF-beta signaling. Inhibits EP300-mediated acetylation of histone H3 at 'Lys-9'. Plays a role in regulating the transcription of AR targets (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223398 | Zfp451 (untagged) - Mouse zinc finger protein 451 (Zfp451), (10ug) |
CNY 13,590.00 |
|
| MR224936 | Zfp451 (Myc-DDK-tagged) - Mouse zinc finger protein 451 (Zfp451) |
CNY 10,550.00 |
|
| MR224936L3 | Lenti ORF clone of Zfp451 (Myc-DDK-tagged) - Mouse zinc finger protein 451 (Zfp451) |
CNY 12,450.00 |
|
| MR224936L4 | Lenti ORF clone of Zfp451 (mGFP-tagged) - Mouse zinc finger protein 451 (Zfp451) |
CNY 12,450.00 |
