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Uhrf1 (NM_001111080) Mouse Tagged ORF Clone
CAT＃: MG226257
- TrueORF®
Uhrf1 (tGFP-tagged) - Mouse ubiquitin-like containing PHD and RING finger domains 1 (Uhrf1) transcript variant 4, (10ug)
ORF Plasmid: DDK
"NM_001111080" in other vectors (3)
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CNY 8,460.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AL022808; ICBP90; Np95; RNF106 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226257 representing NM_001111080
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGATCCAGGTTCGAACTATGGATGGGAAGGAGACCCACACCGTGAACTCTCTGTCCAGGTTGACCA AGGTGCAGGAGCTGAGGAAAAAGATTGAGGAAGTGTTTCACGTGGAACCCCAACTACAGAGACTCTTTTA TAGGGGCAAACAGATGGAGGATGGCCACACACTCTTCGATTATGATGTGCGCCTCAATGACACAATCCAG CTGCTCGTGCGCCAGAGTCTGGCACTGCCTCTCAGTACAAAAGAACGGGACTCGGAGCTCTCAGACTCTG ACTCTGGCTATGGTGTGGGTCACAGTGAATCAGACAAGTCGTCCACGCATGGTGAAGGGGCAGCTGAAGC GGATGACAAGACTGTGTGGGAGGACACGGACCTGGGGCTGTACAAGGTCAATGAGTATGTGGACGTGCGT GACAATATCTTCGGTGCATGGTTTGAGGCCCAGGTGGTCCAGGTACAGAAGAGAGCCCTATCTGAGGACG AGCCCTGTAGCTCCAGTGCCGTTAAGACCTCGGAGGATGACATCATGTACCATGTCAAGTATGATGACTA TCCAGAGCATGGAGTGGACATTGTCAAAGCCAAGAATGTCCGTGCTCGTGCTCGCACTGTGATACCATGG GAGAACCTGGAGGTGGGTCAGGTGGTCATGGCCAACTATAACGTGGACTACCCCAGGAAACGCGGCTTCT GGTATGATGTTGAGATCTGTAGGAAGCGCCAAACCAGGACGGCACGTGAGCTATACGGCAACATCAGGCT CTTGAATGACTCTCAGCTCAACAACTGTCGGATCATGTTTGTGGATGAAGTCTTGATGATTGAGCTCCCT AAAGAGAGGAGGCCTTTGATCGCTAGCCCCTCGCAACGGAAGAGCGGCCCATCCTGCCGGTTCTGCAAGG ATGATGAAAACAAACCATGTCGAAAGTGTGCCTGCCATGTGTGTGGTGGGCGCGAGGCTCCTGAGAAACA GCTGTTGTGTGATGAGTGTGACATGGCCTTCCACCTGTACTGCCTGAAGCCACCGCTCACCTCTGTCCCC CCTGAGCCGGAGTGGTACTGCCCCAGCTGCCGCACTGACTCAAGTGAGGTGGTACAAGCAGGGGAGAAGC TGAAGGAGAGCAAGAAGAAGGCAAAGATGGCATCAGCCACTTCCTCCTCCCGGCGGGACTGGGGCAAGGG CATGGCATGTGTGGGCCGCACCACAGAGTGTACCATTGTGCCCGCCAACCACTTCGGGCCCATCCCTGGT GTCCCTGTGGGCACCATGTGGCGCTTCAGAGTCCAGGTCAGTGAGTCCGGTGTGCATCGGCCTCATGTGG CAGGCATCCATGGCCGGAGCAACGACGGTGCCTACTCATTGGTCCTGGCTGGTGGCTATGAGGATGATGT GGACAATGGCAATTACTTCACATACACAGGGAGTGGTGGCCGAGACCTCTCTGGCAACAAGCGTACAGCA GGCCAGTCCTCTGACCAGAAGCTCACTAATAACAATAGGGCTCTGGCACTCAATTGCCACTCCCCAATCA ATGAGAAAGGTGCGGAGGCTGAAGACTGGCGCCAAGGGAAGCCAGTGCGTGTGGTCCGGAACATGAAGGG CGGGAAACACAGCAAGTACGCTCCTGCAGAGGGCAACCGCTATGATGGCATCTACAAGGTGGTGAAGTAC TGGCCAGAGAGAGGGAAATCTGGCTTCCTCGTGTGGCGTTATCTCCTTCGACGAGATGACACAGAGCCTG AGCCCTGGACCCGGGAGGGCAAGGACCGCACTCGACAGCTGGGGCTCACTATGCAGTACCCTGAAGGCTA CTTGGAGGCCTTGGCTAACAAGGAGAAGAGCAGGAAGCGCCCGGCCAAGGCCTTGGAGCAGGGACCCTCA TCTTCCAAGACAGGCAAAAGCAAGCAGAAGTCCACAGGGCCAACCCTCTCCAGCCCCCGTGCCTCTAAGA AGAGCAAGCTGGAGCCGTACACACTCTCAGAGCAGCAGGCTAACCTCATCAAAGAGGACAAGGGCAACGC CAAGCTGTGGGATGATGTGCTAACTTCACTTCAGGATGGGCCGTACCAGATCTTCCTGAGCAAGGTGAAA GAGGCTTTCCAGTGCATCTGCTGCCAGGAGCTGGTGTTCCGGCCAGTCACCACCGTGTGTCAGCACAACG TCTGTAAGGACTGTCTGGACAGGTCCTTCCGGGCCCAGGTGTTCAGCTGCCCAGCCTGTCGCTTTGAGCT GGACCACAGCTCCCCAACCCGGGTGAACCAGCCCTTGCAGACCATTCTCAACCAGCTCTTCCCTGGCTAT GGCAGCGGCCGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226257 representing NM_001111080
Red=Cloning site Green=Tags(s) MWIQVRTMDGKETHTVNSLSRLTKVQELRKKIEEVFHVEPQLQRLFYRGKQMEDGHTLFDYDVRLNDTIQ LLVRQSLALPLSTKERDSELSDSDSGYGVGHSESDKSSTHGEGAAEADDKTVWEDTDLGLYKVNEYVDVR DNIFGAWFEAQVVQVQKRALSEDEPCSSSAVKTSEDDIMYHVKYDDYPEHGVDIVKAKNVRARARTVIPW ENLEVGQVVMANYNVDYPRKRGFWYDVEICRKRQTRTARELYGNIRLLNDSQLNNCRIMFVDEVLMIELP KERRPLIASPSQRKSGPSCRFCKDDENKPCRKCACHVCGGREAPEKQLLCDECDMAFHLYCLKPPLTSVP PEPEWYCPSCRTDSSEVVQAGEKLKESKKKAKMASATSSSRRDWGKGMACVGRTTECTIVPANHFGPIPG VPVGTMWRFRVQVSESGVHRPHVAGIHGRSNDGAYSLVLAGGYEDDVDNGNYFTYTGSGGRDLSGNKRTA GQSSDQKLTNNNRALALNCHSPINEKGAEAEDWRQGKPVRVVRNMKGGKHSKYAPAEGNRYDGIYKVVKY WPERGKSGFLVWRYLLRRDDTEPEPWTREGKDRTRQLGLTMQYPEGYLEALANKEKSRKRPAKALEQGPS SSKTGKSKQKSTGPTLSSPRASKKSKLEPYTLSEQQANLIKEDKGNAKLWDDVLTSLQDGPYQIFLSKVK EAFQCICCQELVFRPVTTVCQHNVCKDCLDRSFRAQVFSCPACRFELDHSSPTRVNQPLQTILNQLFPGY GSGR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001111080 |
| ORF Size | 2322 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001111080.1, NP_001104550.1 |
| RefSeq Size | 3415 bp |
| RefSeq ORF | 2325 bp |
| Locus ID | 18140 |
| UniProt ID | Q8VDF2 |
| Gene Summary | Multidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. Specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication. In addition to its role in maintenance of DNA methylation, also plays a key role in chromatin modification: through its tudor-like regions and PHD-type zinc fingers, specifically recognizes and binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and unmethylated at 'Arg-2' (H3R2me0), respectively, and recruits chromatin proteins. Enriched in pericentric heterochromatin where it recruits different chromatin modifiers required for this chromatin replication. Also localizes to euchromatic regions where it negatively regulates transcription possibly by impacting DNA methylation and histone modifications. Has E3 ubiquitin-protein ligase activity by mediating the ubiquitination of target proteins such as histone H3 and PML. It is still unclear how E3 ubiquitin-protein ligase activity is related to its role in chromatin in vivo. May be involved in DNA repair.[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR226257 | Uhrf1 (Myc-DDK-tagged) - Mouse ubiquitin-like, containing PHD and RING finger domains, 1 (Uhrf1), transcript variant 4 |
CNY 7,700.00 |
|
| MR226257L3 | Lenti ORF clone of Uhrf1 (Myc-DDK-tagged) - Mouse ubiquitin-like, containing PHD and RING finger domains, 1 (Uhrf1), transcript variant 4 |
CNY 9,600.00 |
|
| MR226257L4 | Lenti ORF clone of Uhrf1 (mGFP-tagged) - Mouse ubiquitin-like, containing PHD and RING finger domains, 1 (Uhrf1), transcript variant 4 |
CNY 9,600.00 |
