United States
Germany
Japan
United Kingdom
China
Mnat1 (NM_008612) Mouse Tagged ORF Clone
CAT＃: MG227103
- TrueORF®
Mnat1 (tGFP-tagged) - Mouse menage a trois 1 (Mnat1), (10ug)
ORF Plasmid: DDK
"NM_008612" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | E130115E11Rik; MAT1; P36 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG227103 representing NM_008612
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGACCAGGGCTGCCCCAGGTGTAAGACCACCAAGTACCGGAACCCTTCTTTGAAGCTGATGGTGA ACGTGTGCGGACACACTCTGTGTGAGAGTTGTGTGGACTTACTGTTTGTAAGAGGAGCTGGAAACTGTCC TGAGTGTGGGACTCCACTGAGAAAGAGCAACTTCAGGGTACAACTCTTTGAAGATCCCACTGTTGATAAG GAGGTTGAAATTCGGAAAAAAGTACTAAAGATATACAATAAAAGGGAAGAAGATTTTCCTAGTTTAAGAG AGTATAATGATTTTCTGGAAGAAGTGGAGGAAATCGTTTTCAATTTGACCAACAATGTGGATTTGGAGAA CACTAAAAAGAAAATGGAAATATATCAAAAAGAAAACAAAGATGTCATTCAGAAAAACAAATTAAAGCTG ACGCGGGAGCAGGAGGAGTTGGAGGAAGCATTGGAGGTAGAACGCCAAGAACATGAGCAGCGACGGCTGT TCATACAAAAGGAAGAAGAGCTGCAGCAGGCTCTGAAAAGGAAGAACAAGCAGGCCTTTTTAGATGAGCT GGAGAGCTCCGACCTCCCTGTTGCTCTGCTCCTGGCGCAGCATAAAGACAGATCGACCCAGCTGGAAATG CAGCTCGAGAAGCCCAGATCCATGAAGCCAGTGACGTTTTCCACAGGAATTAAAATGGGTCAACAGATTT CATTAGCACCAATCCAAAAGCTGGAAGAAGCTCTTTATGAGTACCAGCCCCTGCAGATAGAGACCTGTGG CCCACAAGTTCCTGAGCAAGAGCTCTTAGGACGGCTTGGGTATTTAAACCACGTCCGGGCTGCATCTCCA CAGGATCTTGCTGGCGGCTATACTTCTTCTCTTGCTTGTCACAGAGCGCTACAGGATGCGTTCAGTGGGC TCTTCTGGCAGCCCAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227103 representing NM_008612
Red=Cloning site Green=Tags(s) MDDQGCPRCKTTKYRNPSLKLMVNVCGHTLCESCVDLLFVRGAGNCPECGTPLRKSNFRVQLFEDPTVDK EVEIRKKVLKIYNKREEDFPSLREYNDFLEEVEEIVFNLTNNVDLENTKKKMEIYQKENKDVIQKNKLKL TREQEELEEALEVERQEHEQRRLFIQKEEELQQALKRKNKQAFLDELESSDLPVALLLAQHKDRSTQLEM QLEKPRSMKPVTFSTGIKMGQQISLAPIQKLEEALYEYQPLQIETCGPQVPEQELLGRLGYLNHVRAASP QDLAGGYTSSLACHRALQDAFSGLFWQPR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008612 |
| ORF Size | 927 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008612.2, NP_032638.2 |
| RefSeq Size | 2505 bp |
| RefSeq ORF | 930 bp |
| Locus ID | 17420 |
| UniProt ID | P51949 |
| Gene Summary | Stabilizes the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC208967 | Mnat1 (untagged) - Mouse menage a trois 1 (Mnat1), (10ug) |
CNY 3,990.00 |
|
| MR227103 | Mnat1 (Myc-DDK-tagged) - Mouse menage a trois 1 (Mnat1) |
CNY 2,081.00 |
|
| MR227103L3 | Lenti ORF clone of Mnat1 (Myc-DDK-tagged) - Mouse menage a trois 1 (Mnat1) |
CNY 4,750.00 |
|
| MR227103L4 | Lenti ORF clone of Mnat1 (mGFP-tagged) - Mouse menage a trois 1 (Mnat1) |
CNY 4,750.00 |
