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Pcdh15 (NM_001142738) Mouse Tagged ORF Clone
CAT＃: MG227180
- TrueORF®
Pcdh15 (tGFP-tagged) - Mouse protocadherin 15 (Pcdh15) transcript variant F, (10ug)
ORF Plasmid: DDK
"NM_001142738" in other vectors (2)
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CNY 20,810.00
货期*
9周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | av; BB078305; ENSMUSG00000046980; Gm9815; nmf19; roda; Ush1f |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG227180 representing NM_001142738
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCTACAGTTTGCTGTCTGGAAGTGTTTACCCCATGGGATCCTCATTGCCTCTCTCTTGGTAGTCA GCTGGGGCCAGTATGACGATGATTGCAAACTAGCTAGGGGAGGACCACCAGCTACTATCGTGGCCATTGA TGAAGAGAGTCGAAACGGTACAATTCTGGTGGATAACATGTTGATTAAGGGGACTGCCGGAGGACCAGAC CCCACCATAGAGCTCTCTTTAAAGGACAACGTGGACTACTGGGTGTTGCTGGACCCCGTTAAACAGATGC TTTTCCTGAACAGTACCGGAAGAGTTCTGGATAGAGACCCACCAATGAACATACACTCCATTGTGGTGCA AGTCCAGTGTGTCAACAAGAAGGTTGGCACAGTTATCTATCATGAAGTACGCATCGTGGTGCGAGATCGG AATGACAACTCCCCCACATTCAAGCATGAAAGCTACTATGCCACCGTGAATGAGCTCACTCCAGTTGGCA CCACGATATTCACGGGGTTCTCGGGAGACAATGGAGCTACAGACATAGACGATGGCCCTAATGGACAGAT AGAATACGTGATTCAGTACAACCCAGAAGATCCGGACCGTGCACAAAATCTGAATGAGAGGCGAACAACC ACCACCACCCTCACAGTAGATGTTCTAGATGGAGATGACCTGGGACCTATGTTTCTGCCTTGTGTTCTTG TGCCAAACACACGTGACTGTCGTCCACTCACCTACCAAGCTGCCATTCCTGAACTGAGGACTCCGGAAGA ACTGAACCCTATTTTGGTGACACCACCTATCCAAGCCATTGATCAGGACCGAAACATCCAACCACCATCT GATCGACCTGGCATCCTCTACTCCATCCTTGTCGGCACCCCTGAGGATTACCCCCGCTTCTTCCATATGC ATCCCAGGACTGCAGAACTCACTCTCCTGGAGCCAGTAAACAGAGACTTCCATCAAAAATTTGATTTGGT TATTAAGGCTGAGCAGGACAATGGCCACCCACTTCCTGCCTTTGCTAGTCTGCACATCGAAATACTAGAC GAAAACAATCAGAGTCCATACTTCACAATGCCCAGCTATCAAGGATACATCCTGGAATCCGCCCCAGTGG GAGCCACCATTTCTGAGAGCCTAAACTTAACCACTCCTCTGAGAATTGTAGCTCTGGACAAAGACATAGA AGACACAAAAGATCCAGAGCTCCACCTCTTCCTGAATGACTACACCTCGGTCTTCACTGTGACACCCACT GGTATCACCCGCTACCTCACCCTGCTTCAACCTGTGGACAGGGAGGAACAGCAAACCTACACCTTTCTGA TAACAGCGTTTGATGGCGTGCAAGAAAGTGAGCCAGTCGTGGTCAATATCCGAGTGATGGATGCAAATGA TAACACGCCCACCTTCCCTGAAATCTCCTATGATGTCTATGTTTACACAGACATGAGTCCTGGGGACAGC GTCATTCAGCTGACAGCGGTAGATGCTGATGAAGGCTCTAATGGGGAGATCTCCTATGAAATACTGGTGG GGGGCAAGGGAGACTTCGTGATCAACAAGACCACAGGGCTGGTGAGCATTGCACCAGGCGTGGAGCTGAT CGTGGGACAGACGTATGCGCTCACAGTGCAGGCTTCGGACAACGCCCCGCCTGCAGAAAGAAGGCACTCC ATCTGCACAGTGTACATCGAGGTGCTTCCTCCTAACAACCAGAGCCCTCCCCGCTTCCCGCAGCTGATGT ACAGTCTGGAAGTCAGCGAGGCCATGAGGATCGGTGCTATTTTATTAAATCTACAGGCAACTGATCGAGA GGGAGATCCAATCACATATGCCATCGAGAATGGAGACCCTCAGAGAGTTTTTAATCTTTCAGAAACCACA GGGATTCTCAGCCTAGGGAAGGCTCTAGACCGCGAGAGCACAGACCGCTACATCCTCATCGTCACAGCCT CAGATGGCAGACCGGATGGAACCTCAACTGCCACTGTGAACATAGTGGTGACGGACGTCAATGACAACGC TCCCGTGTTCGATCCCTATCTGCCCAGGAACCTCTCTGTGGTGGAGGAAGAAGCCAATGCCTTTGTGGGT CAAGTCCGGGCAACAGACCCAGATGCTGGGATAAACGGCCAAGTTCACTACAGCCTGGGGAACTTCAACA ACCTCTTCCGCATCACATCCAACGGGAGCATTTACACAGCCGTGAAGCTGAACAGGGAAGCCAGGGACCA CTATGAACTGGTTGTCGTGGCAACAGATGGAGCAGTCCACCCTCGACATTCAACTCTGACACTGTACATC AAGGTGTTGGACATTGATGATAACAGTCCTGTTTTTACCAATTCAACGTACACAGTTGTCGTTGAAGAGA ATCTGCCAGCCGGGACCTCCTTTCTTCAAATAGAGGCCAAGGATGTTGACCTTGGAGCCAATGTGTCATA TCGGATCAGAAGCCCAGAAGTGAAACACCTTTTTGCACTGCATCCATTCACTGGAGAATTGTCTCTTCTG AGGAGTTTGGATTATGAGGCCTTTCCGGACCAGGAGGCAAGCATCACATTCTTGGTGGAGGCCTTTGACA TTTATGGGACTATGCCACCTGGTATAGCAACAGTCACGGTAATTGTGAAGGACATGAATGACTACCCTCC AGTGTTTAGCAAACGCATCTACAAGGGGATGGTGGCTCCAGATGCAGTCAAGGGGACACCAATCACCACC GTTTATGCTGAAGATGCGGACCCACCTGGGATGCCTGCAAGTAGGGTGAGGTATCGAGTGGACGACGTGC AGTTTCCATACCCAGCCAGTATTTTTGATGTAGAGGAAGATTCTGGAAGAGTAGTAACCCGCGTCAATCT TAATGAAGAGCCTACTACGATTTTCAAGCTGGTGGTTGTGGCTTTTGATGACGGCGAACCTGTGATGTCC AGCAGTGCCACGGTGAGAATTCTTGTCTTACATCCTGGAGAGATCCCACGCTTCACCCAAGAGGAATACA GACCTCCTCCTGTAAGTGAGCTTGCGGCCAGAGGGACTGTAGTTGGTGTCATTTCTGCTGCTGCCATTAA TCAGAGCATCGTGTACTCCATTGTGGCAGGAAATGAGGAAGACAAGTTTGGAATCAACAATGTCACTGGG GTCATCTATGTGAATTCACCATTGGATTACGAGACAAGGACCAGCTATGTGCTCCGGGTACAAGCAGATT CTCTGGAAGTGGTCCTTGCCAATCTCCGAGTCCCTTCAAAAAGCAATACAGCTAAGGTGTACATTGAGAT TCAGGATGAAAACGATCACCCCCCAGTGTTCCAGAAGAAATTCTACATTGGAGGTGTGTCTGAAGACGCA AGGATGTTCGCATCTGTGCTCAGAGTGAAGGCCACCGACAGGGACACGGGTAATTACAGTGCCATGGCCT ACCGGCTCATCATACCGCCGATTAAAGAGGGCAAAGAGGGGTTTGTGGTGGAAACATACACAGGTCTCAT CAAGACAGCCATGCTCTTCCACAATATGAGAAGATCCTACTTCAAGTTTCAAGTGATTGCAACTGACGAC TACGGGAAGGGGTTGAGCGGGAAAGCAGACGTACTGGTCTCCGTGGTCAATCAACTGGATATGCAGGTCA TTGTCTCCAATGTGCCCCCTACACTAGTGGAAAAGAAGATAGAAGACCTTACAGAGATTTTGGATCGCTA CGTTCAGGAGCAAATTCCTGGTGCCAAGGTTGTGGTGGAGTCCATAGGTGCCCGTCGCCATGGAGACGCC TACTCCCTAGAAGACTATAGCAAGTGCGACCTGACTGTCTATGCCATCGACCCGCAGACCAACAGAGCCA TCGACAGAAATGAGCTTTTTAAGTTCCTGGACGGCAAACTGCTCGATATCAATAAAGACTTCCAGCCGTA TTACGGGGAAGGAGGGCGCATTCTGGAGATTCGGACACCTGAGGCAGTGACGAGCATCAAGAAGCGAGGA GAAAGCTTGGGGTACACAGAAGGGGCCTTGCTGGCCTTGGCCTTCATCATCATCCTCTGTTGCATCCCAG CCATCTTGGTCGTCTTAGTAAGCTACCGACAACGCCAGGCTGAGTGCACGAAGACCGCAAGAATTCAGTC TGCTATGCCTGCAGCCAAGCCTGCAGCTCCTGTACCAGCTGCGCCTGCGCCGCCCCCGCCCCCGCCACCA CCACCACCAGGAGCACATCTCTATGAAGAACTGGGAGAGAGCGCAATTCTTTTCCTTCTCTACCATTTTG AACAAAGCAGGGGAAATAACTCAGTCCCAGAAGACAGGAGCAGTCATCGCGATGGGATGGCCTTTTCCTC CAGTACCACTGAGTCTCATGAGCCAGCTCATGTAGAGGGACCACTTAAGGAGAGCCAGCCTAACCCAGCA AGGACGTTCTCATTTGTTCCTGATGAGGATAACTTAAGTACCCATAATCCCCTTTACATGGAAAGTATAG GTCAAAGGTCAACAAACTCAGACCTTCAGCCACGAACAGATTTTGAAGAGCTGTTGGCACCCAGAACACA AGTTAAGAGTCAGTCTCTGAGGGGCCCAAGAGAAAAGATCCAGAGGGTGTGGAATCAGTCTGTGAGCTTT CCTAGGCGGCTCATGTGGAAAGCCCCAAACAGGCCAGAGACCATAGACCTGGTGGAGTGGCAGATCACCA ATCAGAGAGCTGAATGCGAAAGCGCCAGATGCCACCCAAGCCAGAGAGGTAGCAGCAACGTTCTGCTGGC AACTGAAGATGCCCACGAGTCAGAGAAAGAAGGGGGACACAGAGACACCCTAATCGTCCAGCAAACAGAG CAGCTGAAATCTCTGTCTTCTGGCTCTTCTTTTTCCTCCTCTTGGTCTCACTTTTCTTTCTCAACTCTGC CAACGATTTCCAGAGCGGTGGAACTCGGGTCGGAACCTAATGTGGTCACTTCTCCCGCTGACTGCACCTT GGAACTTTCTCCTCCTCTGAGACCCCGTATTTTAAACTCCTTAAGCTCTAAGAGAGAGACTCCCACATGT GCATCAGATACAGAACCAAAAAGGAACTCTTTTGAGATCGCTCCCCATCCACCTAGCATCTCTGCTCCCC TCCCACATCCGCCTCTTCCTAGACCTCCCATTGCCTTTACCACTTTTCCTCTTCCCCTTTCTCCCCCTAA CCCTCCTCCCCCACAACTTGTTACATTTTCTCTTCCCATTTCTACACCCCCTACTTCTTCTCTACCTCTT CCTCCTCCACTGTCACTTCCTCCTCCTCCTCGGCCACCAGCTCCCCGCCTCTTCCCACAGCCTCCTTCCA CGTCCATTCCATCCACAGACAGCATCTCTGCACCAGCTGCTAAATGCACTGCCAGTGCCACACACGCCAG AGAAACCACGTCTACGACACAGCCACCAGCATCCAACCCGCAGTGGGGGGCAGAACCCCACAGACATCCA AAAGGGATCCTCAGACATGTGAAAAACTTGGCAGAGCTCGAGAAATCAGTGTCTAACATGTACAGTCACA TAGAAAAAAACTGCCCACCTGCAGATCCCTCAAAACTACACACGTTTTGCCCTGCAGAGAAAACAGGCAT GAAAATCACACATGACCAGAGCCAGGAAACGTTGGTTAGAGTTGTTGAGGGAATTGACGTGCAACCTCAC AGTCAATCAACATCTTTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227180 representing NM_001142738
Red=Cloning site Green=Tags(s) MFLQFAVWKCLPHGILIASLLVVSWGQYDDDCKLARGGPPATIVAIDEESRNGTILVDNMLIKGTAGGPD PTIELSLKDNVDYWVLLDPVKQMLFLNSTGRVLDRDPPMNIHSIVVQVQCVNKKVGTVIYHEVRIVVRDR NDNSPTFKHESYYATVNELTPVGTTIFTGFSGDNGATDIDDGPNGQIEYVIQYNPEDPDRAQNLNERRTT TTTLTVDVLDGDDLGPMFLPCVLVPNTRDCRPLTYQAAIPELRTPEELNPILVTPPIQAIDQDRNIQPPS DRPGILYSILVGTPEDYPRFFHMHPRTAELTLLEPVNRDFHQKFDLVIKAEQDNGHPLPAFASLHIEILD ENNQSPYFTMPSYQGYILESAPVGATISESLNLTTPLRIVALDKDIEDTKDPELHLFLNDYTSVFTVTPT GITRYLTLLQPVDREEQQTYTFLITAFDGVQESEPVVVNIRVMDANDNTPTFPEISYDVYVYTDMSPGDS VIQLTAVDADEGSNGEISYEILVGGKGDFVINKTTGLVSIAPGVELIVGQTYALTVQASDNAPPAERRHS ICTVYIEVLPPNNQSPPRFPQLMYSLEVSEAMRIGAILLNLQATDREGDPITYAIENGDPQRVFNLSETT GILSLGKALDRESTDRYILIVTASDGRPDGTSTATVNIVVTDVNDNAPVFDPYLPRNLSVVEEEANAFVG QVRATDPDAGINGQVHYSLGNFNNLFRITSNGSIYTAVKLNREARDHYELVVVATDGAVHPRHSTLTLYI KVLDIDDNSPVFTNSTYTVVVEENLPAGTSFLQIEAKDVDLGANVSYRIRSPEVKHLFALHPFTGELSLL RSLDYEAFPDQEASITFLVEAFDIYGTMPPGIATVTVIVKDMNDYPPVFSKRIYKGMVAPDAVKGTPITT VYAEDADPPGMPASRVRYRVDDVQFPYPASIFDVEEDSGRVVTRVNLNEEPTTIFKLVVVAFDDGEPVMS SSATVRILVLHPGEIPRFTQEEYRPPPVSELAARGTVVGVISAAAINQSIVYSIVAGNEEDKFGINNVTG VIYVNSPLDYETRTSYVLRVQADSLEVVLANLRVPSKSNTAKVYIEIQDENDHPPVFQKKFYIGGVSEDA RMFASVLRVKATDRDTGNYSAMAYRLIIPPIKEGKEGFVVETYTGLIKTAMLFHNMRRSYFKFQVIATDD YGKGLSGKADVLVSVVNQLDMQVIVSNVPPTLVEKKIEDLTEILDRYVQEQIPGAKVVVESIGARRHGDA YSLEDYSKCDLTVYAIDPQTNRAIDRNELFKFLDGKLLDINKDFQPYYGEGGRILEIRTPEAVTSIKKRG ESLGYTEGALLALAFIIILCCIPAILVVLVSYRQRQAECTKTARIQSAMPAAKPAAPVPAAPAPPPPPPP PPPGAHLYEELGESAILFLLYHFEQSRGNNSVPEDRSSHRDGMAFSSSTTESHEPAHVEGPLKESQPNPA RTFSFVPDEDNLSTHNPLYMESIGQRSTNSDLQPRTDFEELLAPRTQVKSQSLRGPREKIQRVWNQSVSF PRRLMWKAPNRPETIDLVEWQITNQRAECESARCHPSQRGSSNVLLATEDAHESEKEGGHRDTLIVQQTE QLKSLSSGSSFSSSWSHFSFSTLPTISRAVELGSEPNVVTSPADCTLELSPPLRPRILNSLSSKRETPTC ASDTEPKRNSFEIAPHPPSISAPLPHPPLPRPPIAFTTFPLPLSPPNPPPPQLVTFSLPISTPPTSSLPL PPPLSLPPPPRPPAPRLFPQPPSTSIPSTDSISAPAAKCTASATHARETTSTTQPPASNPQWGAEPHRHP KGILRHVKNLAELEKSVSNMYSHIEKNCPPADPSKLHTFCPAEKTGMKITHDQSQETLVRVVEGIDVQPH SQSTSL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001142738 |
| ORF Size | 5688 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001142738.1, NP_001136210.1 |
| RefSeq Size | 8923 bp |
| RefSeq ORF | 5691 bp |
| Locus ID | 11994 |
| UniProt ID | Q99PJ1 |
| Gene Summary | Calcium-dependent cell-adhesion protein. Required for inner ear neuroepithelial cell elaboration and cochlear function. Probably involved in the maintenance of normal retinal function.[UniProtKB/Swiss-Prot Function] |
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