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Stx12 (NM_133887) Mouse Tagged ORF Clone
CAT＃: MR203669
- TrueORF®
Stx12 (Myc-DDK-tagged) - Mouse syntaxin 12 (Stx12)
ORF Plasmid: tGFP
"NM_133887" in other vectors (4)
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CNY 1,416.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI850350; AU041521; Stx13 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR203669 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCTACGGTCCCTTAGACATGTATCGGAACCCCGGGCCGTCGGGGCCTCAGCCCCGGGACTTCAACA GCATCATCCAGACATGCAGTGGCAACATCCAGCGGATCAGCCAAGCCACTGCTCAGATAAAGAATTTGAT GAGCCAACTTGGAACTAAGCAGGACTCAAGCAAACTCCAGGAAAATTTGCAACAGTTACAGCACTCCACA AATCAGCTGGCCAAGGAAACAAATGAGTTGCTGAAGGAATTAGGGTCCTTGCCCCTACCCTTGTCTGCAT CAGAGCAGCGCCAGCAGAAGCTTCAGAAGGAGCGGCTCATGAATGACTTCTCGTCGGCCTTAAACAATTT CCAGGTTGTGCAGAGAAAGGTATCTGAGAAGGAAAAGGAGAGCATCGCGAGAGCGAGAGCCGGATCTCGT CTTTCTGCAGAAGACAGGCAGAGAGAAGAGCAGCTCGTCTCATTTGACAGCCATGAAGAGTGGAACCAAA TGCAGAGCCAGGAGGAAGAGGTGGCCATCACTGAGCAAGACCTGGAACTTATCAAAGAGAGGGAAACGGC GATTCGGCAGCTGGAGGCTGACATTTTGGATGTCAATCAGATATTTAAAGATTTAGCTATGATGATCCAT GACCAAGGTGATCTGATCGATAGCATAGAAGCCAATGTGGAAAGCTCGGAGGTGCATGTGGAAAGAGCCA CTGATCAGCTACAAAGAGCTGCTTACTATCAGAAAAAATCTCGCAAGAAGATGTGTATCCTCGTGCTTGT CCTGTCAGTGATTGTTACAGTCTTGGTAGTTGTTATCTGGGTTGCTTCTAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203669 protein sequence
Red=Cloning site Green=Tags(s) MSYGPLDMYRNPGPSGPQPRDFNSIIQTCSGNIQRISQATAQIKNLMSQLGTKQDSSKLQENLQQLQHST NQLAKETNELLKELGSLPLPLSASEQRQQKLQKERLMNDFSSALNNFQVVQRKVSEKEKESIARARAGSR LSAEDRQREEQLVSFDSHEEWNQMQSQEEEVAITEQDLELIKERETAIRQLEADILDVNQIFKDLAMMIH DQGDLIDSIEANVESSEVHVERATDQLQRAAYYQKKSRKKMCILVLVLSVIVTVLVVVIWVASK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133887 |
| ORF Size | 825 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133887.1, NM_133887.2, NM_133887.3, NM_133887.4, NP_598648.1 |
| RefSeq Size | 2320 bp |
| RefSeq ORF | 825 bp |
| Locus ID | 100226 |
| UniProt ID | Q9ER00 |
| MW | 31.2 kDa |
| Gene Summary | SNARE that acts to regulate protein transport between late endosomes and the trans-Golgi network. The SNARE complex containing STX6, STX12, VAMP4 and VTI1A mediates vesicle fusion (in vitro) (By similarity). Through complex formation with GRIP1, GRIA2 and NSG1 controls the intracellular fate of AMPAR and the endosomal sorting of the GRIA2 subunit toward recycling and membrane targeting (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC207797 | Stx12 (untagged) - Mouse syntaxin 12 (Stx12), (10ug) |
CNY 2,640.00 |
|
| MG203669 | Stx12 (tGFP-tagged) - Mouse syntaxin 12 (Stx12) |
CNY 2,850.00 |
|
| MR203669L3 | Lenti ORF clone of Stx12 (Myc-DDK-tagged) - Mouse syntaxin 12 (Stx12) |
CNY 4,750.00 |
|
| MR203669L4 | Lenti ORF clone of Stx12 (mGFP-tagged) - Mouse syntaxin 12 (Stx12) |
CNY 4,750.00 |
