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Klf15 (NM_023184) Mouse Tagged ORF Clone
CAT＃: MR206548
- TrueORF®
Klf15 (Myc-DDK-tagged) - Mouse Kruppel-like factor 15 (Klf15)
ORF Plasmid: tGFP
"NM_023184" in other vectors (4)
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CNY 2,850.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1810013I09Rik; AV048136; AW494632; CKLF; KKLF |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206548 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGACCACCTGCTTCCAGTGGACGAGACCTTCTCGTCACCGAAATGCTCAGTGGGTTACCTAGGGG ACAGGCTGGCCAGCCGGCAGCCATACCACATGTTGCCCTCGCCCATCTCGGAGGATGACAGCGATGTCTC CAGCCCCTGCTCTTGTGCCAGCCCTGACTCGCAAGCCTTCTGTTCCTGCTACAGTGCGGGTCCAGGCCCT GAGGCCCAGGGCAGCATCTTGGATTTCCTCCTGTCCCGGGCCACACTGGGCAGTGGTGGTGGCAGTGGAG GTATTGGAGATAGCAGTGGCCCTGTGACCTGGGGATCATGGAGGAGAGCCTCTGTGCCTGTGAAGGAGGA ACATTTCTGCTTCCCTGAATTTCTGTCAGGGGACACTGATGACGTCTCCAGGCCCTTCCAGCCTACCCTG GAGGAGATTGAAGAATTCCTGGAAGAGAACATGGAGGCTGAGGTCAAGGAGGCCCCAGAGAACGGTAGCA GGGACCTGGAGACCTGTAGCCAGCTCTCAGCTGGGTCACACCGGAGCCACCTTCATCCAGAGTCTGCTGG GAGAGAGCGCTGTACCCCACCACCAGGTGGCACGAGTGGGGGTGGTGCCCAAAGTGCAGGTGAGGGGCCA GCACATGATGGCCCCGTGCCGGTGCTACTGCAGATCCAGCCTGTTGCTGTGAAGCAGGAGGCAGGTACAG GGCCAGCCTCCCCAGGGCAGGCCCCAGAGAGCGTCAAGGTCGCCCAGCTTCTAGTCAACATCCAGGGGCA GACCTTTGCACTCCTGCCTCAAGTGGTACCATCCTCCAACTTGAACCTGCCCTCAAAGTTTGTGCGAATT GCGCCTGTGCCCATTGCCGCCAAACCTATTGGCTCAGGATCCCTAGGGCCCGGCCCTGCTGGCCTCCTTG TGGGCCAGAAGTTTCCCAAGAACCCAGCAGCAGAACTTCTCAAAATGCACAAATGCACTTTCCCAGGCTG CAGCAAGATGTACACCAAGAGCAGCCACCTCAAGGCCCACCTGCGTCGGCACACAGGCGAGAAGCCCTTT GCCTGCACCTGGCCAGGCTGCGGCTGGAGGTTTTCCCGCTCAGATGAGTTGTCAAGGCACCGGCGATCTC ACTCGGGTGTGAAGCCGTACCAGTGTCCCGTGTGCGAGAAGAAATTCGCGCGGAGTGACCACCTCTCCAA ACACATCAAAGTGCATCGCTTCCCACGAAGCAGCCGCGCAGTACGCGCCATCAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206548 protein sequence
Red=Cloning site Green=Tags(s) MVDHLLPVDETFSSPKCSVGYLGDRLASRQPYHMLPSPISEDDSDVSSPCSCASPDSQAFCSCYSAGPGP EAQGSILDFLLSRATLGSGGGSGGIGDSSGPVTWGSWRRASVPVKEEHFCFPEFLSGDTDDVSRPFQPTL EEIEEFLEENMEAEVKEAPENGSRDLETCSQLSAGSHRSHLHPESAGRERCTPPPGGTSGGGAQSAGEGP AHDGPVPVLLQIQPVAVKQEAGTGPASPGQAPESVKVAQLLVNIQGQTFALLPQVVPSSNLNLPSKFVRI APVPIAAKPIGSGSLGPGPAGLLVGQKFPKNPAAELLKMHKCTFPGCSKMYTKSSHLKAHLRRHTGEKPF ACTWPGCGWRFSRSDELSRHRRSHSGVKPYQCPVCEKKFARSDHLSKHIKVHRFPRSSRAVRAIN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_023184 |
| ORF Size | 1248 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_023184.4 |
| RefSeq Size | 2294 bp |
| RefSeq ORF | 1248 bp |
| Locus ID | 66277 |
| UniProt ID | Q9EPW2 |
| MW | 44.3 kDa |
| Gene Summary | Transcriptional regulator that binds to the GA element of the CLCNKA promoter (By similarity). Binds to the KCNIP2 promoter and regulates KCNIP2 circadian expression in the heart. Is a repressor of CCN2 expression, involved in the control of cardiac fibrosis. Is also involved in the control of cardiac hypertrophy acting through the inhibition of MEF2A, GATA4 and MYOCD activity. Is a negative regulator of TP53 acetylation. Inhibits NF-kappa-B activation through repression of EP300-dependent RELA acetylation (By similarity). Involved in podocyte differentiation.[UniProtKB/Swiss-Prot Function] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Pulsed glucocorticoids enhance dystrophic muscle performance through epigenetic-metabolic reprogramming
,Quattrocelli, M;Zelikovich, AS;Jiang, Z;Peek, CB;Demonbreun, AR;Kuntz, NL;Barish, GD;Haldar, SM;Bass, J;McNally, EM;,
JCI Insight
,PubMed ID 31852847
[KLF15]
|
|
Glucose promotes cell growth by suppressing branched-chain amino acid degradation
,Shao, D;Villet, O;Zhang, Z;Choi, SW;Yan, J;Ritterhoff, J;Gu, H;Djukovic, D;Christodoulou, D;Kolwicz, SC;Raftery, D;Tian, R;,
Nat Commun
,PubMed ID 30050148
[KLF15]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205755 | Klf15 (untagged) - Mouse Kruppel-like factor 15 (Klf15), (10ug) |
CNY 2,000.00 |
|
| MG206548 | Klf15 (tGFP-tagged) - Mouse Kruppel-like factor 15 (Klf15) |
CNY 3,140.00 |
|
| MR206548L3 | Lenti ORF clone of Klf15 (Myc-DDK-tagged) - Mouse Kruppel-like factor 15 (Klf15) |
CNY 4,750.00 |
|
| MR206548L4 | Lenti ORF clone of Klf15 (mGFP-tagged) - Mouse Kruppel-like factor 15 (Klf15) |
CNY 4,750.00 |
