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Errfi1 (NM_133753) Mouse Tagged ORF Clone
CAT＃: MR207372
- TrueORF®
Errfi1 (Myc-DDK-tagged) - Mouse ERBB receptor feedback inhibitor 1 (Errfi1)
ORF Plasmid: tGFP
"NM_133753" in other vectors (4)
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CNY 3,330.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1300002F13Rik; AI788755; Mig-6; Mig6; RALT |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207372 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAACAGCAGGAGTTGCTGCTCAGGATATTCGAGTCCCATTAAAAACTGGATTTCTCCATAATGGTC AGGCCTTGGGGAATATGAAGTCCTGCTGGGGCAGTCACAGTGAGTTTGAAAATAACTTTTTAAATATTGA TCCAATAACCATGGCCTACAATCTGAACTCCCCTGCTCAGGAGCACCTAACAACTGTTGGATGTGCTGCT CGGTCTGCTCCAGGGAGCGGCCACTTCTTTGCAGAGTGTGGTCCATCTCCAAGGTCAAGCTTGCCCCCTC TTGTTATCTCACCAAGTGAAAGCTCGGGACAGCGTGAAGAGGATCAAGTTATGTGTGGTTTTAAGAAACT CTCAGTGAATGGGGTCTGCACTTCCACACCTCCACTTACACCCATTAAAAGCTGCCCTTCCCCTTTCCCC TGTGCGGCTCTGTGTGATCGGGGTTCTCGGCCGCTCCCGCCACTGCCCATCTCTGAAGACCTATGTGTGG ATGAGGCCGACAGTGAGGTAGAGCTTCTAACCACCAGCTCAGACACAGACTTGCTTTTAGAAGACTCTGC GCCTTCAGATTTCAAATACGATGCTCCTGGCAGGCGCAGCTTCCGTGGGTGCGGCCAGATCAACTATGCA TATTTTGACAGCCCAACTGTTTCTGTGGCAGATCTTAGCTGTGCATCTGACCAGAACAGAGTTGTTCCAG ACCCAAACCCTCCCCCACCTCAAAGCCATCGCAGATTAAGGAGGTCTCACTCAGGACCAGCTGGGTCATT TAACAAGCCAGCCATTCGGATATCTAGCTGCACACACAGAGCTTCTCCTAGCTCTGATGAAGACAAGCCT GAGGTCCCTCCCAGGGTTCCTATACCTCCTAGGCCAGCAAAGCCAGACTATAGACGGTGGTCAGCAGAAG TGACCTCCAACACCTACAGTGATGAAGATAGGCCTCCCAAAGTCCCCCCGAGAGAACCTTTGTCTCGGAG TAACTCCCGTACCCCAAGTCCTAAAAGCCTTCCGTCTTACCTCAATGGGGTCATGCCCCCAACACAGAGC TTCGCTCCTGACCCCAAGTATGTCAGCAGCAAAGCCCTGCAGAGACAGAGCAGCGAAGGATCTGCCAACA AGGTTCCTTGCATCCTGCCCATTATTGAAAATGGGAAGAAGGTTAGCTCAACGCATTATTACTTACTACC TGAGAGGCCACCGTACCTGGACAAATATGAAAAGTATTTTAAGGAAGCAGAAGAAACAAACCCAAGCACC CAAATTCAGCCATTACCTGCTGCCTGTGGTATGGCCTCTGCCACAGAAAAGCTGGCCTCCAGAATGAAAA TAGATATGGGTAGCCACGGGAAGCGCAAACACTTATCCTACGTGGTTTCTCCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207372 protein sequence
Red=Cloning site Green=Tags(s) MSTAGVAAQDIRVPLKTGFLHNGQALGNMKSCWGSHSEFENNFLNIDPITMAYNLNSPAQEHLTTVGCAA RSAPGSGHFFAECGPSPRSSLPPLVISPSESSGQREEDQVMCGFKKLSVNGVCTSTPPLTPIKSCPSPFP CAALCDRGSRPLPPLPISEDLCVDEADSEVELLTTSSDTDLLLEDSAPSDFKYDAPGRRSFRGCGQINYA YFDSPTVSVADLSCASDQNRVVPDPNPPPPQSHRRLRRSHSGPAGSFNKPAIRISSCTHRASPSSDEDKP EVPPRVPIPPRPAKPDYRRWSAEVTSNTYSDEDRPPKVPPREPLSRSNSRTPSPKSLPSYLNGVMPPTQS FAPDPKYVSSKALQRQSSEGSANKVPCILPIIENGKKVSSTHYYLLPERPPYLDKYEKYFKEAEETNPST QIQPLPAACGMASATEKLASRMKIDMGSHGKRKHLSYVVSP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133753 |
| ORF Size | 1386 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133753.2 |
| RefSeq Size | 3034 bp |
| RefSeq ORF | 1386 bp |
| Locus ID | 74155 |
| UniProt ID | Q99JZ7 |
| MW | 50.1 kDa |
| Gene Summary | Negative regulator of EGFR signaling in skin morphogenesis. Acts as a negative regulator for several EGFR family members, including ERBB2, ERBB3 and ERBB4. Inhibits EGFR catalytic activity by interfering with its dimerization. Inhibits autophosphorylation of EGFR, ERBB2 and ERBB4. Important for normal keratinocyte proliferation and differentiation. Plays a role in modulating the response to steroid hormones in the uterus. Required for normal response to progesterone in the uterus and for fertility. Mediates epithelial estrogen responses in the uterus by regulating ESR1 levels and activation. Important for regulation of endometrium cell proliferation. Important for normal prenatal and perinatal lung development.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC216090 | Errfi1 (untagged) - Mouse ERBB receptor feedback inhibitor 1 (Errfi1), (10ug) |
CNY 5,990.00 |
|
| MG207372 | Errfi1 (tGFP-tagged) - Mouse ERBB receptor feedback inhibitor 1 (Errfi1) |
CNY 3,710.00 |
|
| MR207372L3 | Lenti ORF clone of Errfi1 (Myc-DDK-tagged) - Mouse ERBB receptor feedback inhibitor 1 (Errfi1) |
CNY 5,230.00 |
|
| MR207372L4 | Lenti ORF clone of Errfi1 (mGFP-tagged) - Mouse ERBB receptor feedback inhibitor 1 (Errfi1) |
CNY 5,230.00 |
