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Pak3 (NM_001195049) Mouse Tagged ORF Clone
CAT＃: MR208677
- TrueORF®
Pak3 (Myc-DDK-tagged) - Mouse p21 protein (Cdc42/Rac)-activated kinase 3 (Pak3), transcript variant 5
"NM_001195049" in other vectors (2)
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CNY 4,056.00
CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PAK-3; Pak65alpha; Pak65beta; Stk4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208677 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGACAGCTTGGATAACGAAGAAAAACCCCCAGCTCCCCCACTGAGGATGAACAGTAACAACCGAG ACTCTTCAGCACTCAACCACAGCTCCAAACCACTGCCCATGGCCCCGGAAGAGAAGAATAAGAAAGCCAG GCTTCGCTCTATCTTCCCAGGAGGAGGGGATAAAACCAATAAGAAGAAAGAGAAAGAGCGCCCAGAGATC TCTCTTCCTTCAGACTTTGAGCATACGATTCATGTGGGTTTTGATGCAGTCACCGGGGAATTCACTGGAA TTCCTGAGCAATGGGCACGACTACTCCAAACCTCCAACATAACAAAGCTGGAACAGAAGAAGAACCCACA AGCTGTTCTTGATGTTCTCAAGTTCTACGACTCCAAAGAAACGGTCAACAACCAGAAATATATGAGCTTT ACGTCAGGAGATAAAAGTGCCCATGGATACATAGCAGCACATCAGTCGAATACCAAAACAGCTTCGGAAC CTCCTTTGGCTCCTCCTGTATCTGAAGAAGAGGATGAAGAAGAGGAAGAGGAAGAAGATGACAATGAACC TCCGCCTGTCATTGCACCAAGACCAGAGCATACAAAATCAATCTATACTCGCTCCGTGGTTGAGTCAATT GCTTCACCAGCTGCACCAAATAAAGAAGATATCCCACCTTCTGCTGAGAATGCCAATTCCACCACTTTGT ACAGGAATACAGATCGGCAAAGAAAAAAGTCCAAGATGACGGATGAGGAGATCCTAGAGAAGCTGAGAAG CATTGTGAGTGTTGGGGACCCAAAGAAGAAATATACGAGATTTGAAAAAATTGGCCAAGGGGCATCAGGA ACTGTTTACACAGCACTAGACATTGCGACTGGACAAGAGGTGGCCATAAAGCAAATGAACCTTCAACAGC AGCCCAAAAAGGAATTAATTATTAATGAAATTCTTGTCATGAGGGAAAATAAGAACCCCAATATTGTCAA TTATTTAGATAGCTACTTAGTGGGTGATGAACTGTGGGTAGTCATGGAATACTTGGCTGGTGGCTCTTTG ACTGATGTGGTCACAGAAACCTGTATGGATGAAGGACAGATAGCAGCTGTCTGTAGAGAGTGCCTCCAAG CTTTGGATTTCTTGCACTCAAACCAAGTGATCCACAGAGACATAAAGAGCGACAACATTCTCCTCGGGAT GGATGGTTCTGTTAAACTGACTGATTTTGGGTTCTGTGCTCAAATCACTCCTGAGCAAAGTAAACGAAGC ACTATGGTGGGAACTCCCTATTGGATGGCACCTGAAGTGGTAACTCGAAAAGCTTATGGTCCAAAAGTTG ATATCTGGTCTCTGGGAATCATGGCCATTGAAATGGTGGAAGGTGAACCCCCTTACCTTAATGAAAATCC ACTCAGGGCCTTATATCTGATAGCCACTAATGGGACCCCAGAGCTCCAGAATCCTGAGAGACTGTCAGCT GTATTCCGTGACTTCTTAAATCGCTGTCTTGAGATGGATGTGGATAGAAGAGGATCTGCTAAGGAGCTTT TGCAGCATCCGTTTTTAAAATTAGCCAAGCCTCTGTCTAGTCTCACTCCTCTGATTATCGCCGCAAAGGA AGCAATTAAGAACAGTAGCCGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208677 protein sequence
Red=Cloning site Green=Tags(s) MSDSLDNEEKPPAPPLRMNSNNRDSSALNHSSKPLPMAPEEKNKKARLRSIFPGGGDKTNKKKEKERPEI SLPSDFEHTIHVGFDAVTGEFTGIPEQWARLLQTSNITKLEQKKNPQAVLDVLKFYDSKETVNNQKYMSF TSGDKSAHGYIAAHQSNTKTASEPPLAPPVSEEEDEEEEEEEDDNEPPPVIAPRPEHTKSIYTRSVVESI ASPAAPNKEDIPPSAENANSTTLYRNTDRQRKKSKMTDEEILEKLRSIVSVGDPKKKYTRFEKIGQGASG TVYTALDIATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGDELWVVMEYLAGGSL TDVVTETCMDEGQIAAVCRECLQALDFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRS TMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQNPERLSA VFRDFLNRCLEMDVDRRGSAKELLQHPFLKLAKPLSSLTPLIIAAKEAIKNSSR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001195049 |
| ORF Size | 1635 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001195049.1, NP_001181978.1 |
| RefSeq Size | 8616 bp |
| RefSeq ORF | 1635 bp |
| Locus ID | 18481 |
| UniProt ID | Q61036 |
| MW | 60.8 kDa |
| Gene Summary | Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity (PubMed:25851601). Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR208677L3 | Lenti ORF clone of Pak3 (Myc-DDK-tagged) - Mouse p21 protein (Cdc42/Rac)-activated kinase 3 (Pak3), transcript variant 5 |
CNY 6,460.00 |
|
| MR208677L4 | Lenti ORF clone of Pak3 (mGFP-tagged) - Mouse p21 protein (Cdc42/Rac)-activated kinase 3 (Pak3), transcript variant 5 |
CNY 6,460.00 |
