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Mmp2 (NM_008610) Mouse Tagged ORF Clone
CAT＃: MR209857
- TrueORF®
Mmp2 (Myc-DDK-tagged) - Mouse matrix metallopeptidase 2 (Mmp2)
ORF Plasmid: tGFP
"NM_008610" in other vectors (4)
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CNY 4,936.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Clg; Clg4a; Ge; GelA; MMP-; MMP-2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209857 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCACGAGTGGCCTGGGGAGCGCTGGCCGGACCTCTGCGGGTTCTCTGCGTCCTGTGCTGCCTGT TGGGCCGCGCCATCGCTGCACCATCGCCCATCATCAAGTTCCCCGGCGATGTCGCCCCTAAAACAGACAA AGAGTTGGCAGTGCAATACCTGAACACTTTCTATGGCTGCCCCAAGGAGAGTTGCAACCTCTTTGTGCTG AAAGATACCCTCAAGAAGATGCAGAAGTTCTTTGGGCTGCCCCAGACAGGTGACCTTGACCAGAACACCA TCGAGACCATGCGGAAGCCAAGATGTGGCAACCCAGATGTGGCCAACTACAACTTCTTCCCCCGCAAGCC CAAGTGGGACAAGAACCAGATCACATACAGGATCATTGGTTACACACCTGACCTGGACCCTGAAACCGTG GATGATGCTTTTGCTCGGGCCTTAAAAGTATGGAGCGACGTCACTCCGCTGCGCTTTTCTCGAATCCATG ATGGGGAGGCTGACATCATGATCAACTTTGGACGCTGGGAGCATGGAGATGGATACCCATTTGATGGCAA GGATGGACTCCTGGCACATGCCTTTGCCCCGGGCACTGGTGTTGGGGGAGATTCTCACTTTGATGATGAT GAGCTGTGGACCCTGGGAGAAGGACAAGTGGTCCGCGTAAAGTATGGGAACGCTGATGGCGAGTACTGCA AGTTCCCCTTCCTGTTCAACGGTCGGGAATACAGCAGCTGTACAGACACTGGTCGCAGTGATGGCTTCCT CTGGTGCTCCACCACATACAACTTTGAGAAGGATGGCAAGTATGGCTTCTGCCCCCATGAAGCCTTGTTT ACCATGGGTGGCAATGCAGATGGACAGCCCTGCAAGTTCCCGTTCCGCTTCCAGGGCACCTCCTACAACA GCTGTACCACCGAGGGCCGCACCGATGGCTACCGCTGGTGTGGCACCACCGAGGACTATGACCGGGATAA GAAGTATGGATTCTGTCCCGAGACCGCTATGTCCACTGTGGGTGGAAATTCAGAAGGTGCCCCATGTGTC TTCCCCTTCACTTTCCTGGGCAACAAGTATGAGAGCTGCACCAGCGCCGGCCGCAACGATGGCAAGGTGT GGTGTGCGACCACAACCAACTACGATGATGACCGGAAGTGGGGCTTCTGTCCTGACCAAGGATATAGCCT ATTCCTCGTGGCAGCCCATGAGTTCGGCCATGCCATGGGGCTGGAACACTCTCAGGACCCTGGAGCTCTG ATGGCCCCGATCTACACCTACACCAAGAACTTCCGATTATCCCATGATGACATCAAGGGGATCCAGGAGC TCTATGGGCCCTCCCCCGATGCTGATACTGACACTGGTACTGGCCCCACACCAACACTGGGACCTGTCAC TCCGGAGATCTGCAAACAGGACATTGTCTTTGATGGCATCGCTCAGATCCGTGGTGAGATCTTCTTCTTC AAGGACCGGTTTATTTGGCGGACAGTGACACCACGTGACAAGCCCACAGGTCCCTTGCTGGTGGCCACAT TCTGGCCTGAGCTCCCAGAAAAGATTGACGCTGTGTATGAGGCCCCACAGGAGGAGAAGGCTGTGTTCTT CGCAGGGAATGAGTACTGGGTCTATTCTGCTAGTACTCTGGAGCGAGGATACCCCAAGCCACTGACCAGC CTGGGGTTGCCCCCTGATGTCCAGCAAGTAGATGCTGCCTTTAACTGGAGTAAGAACAAGAAGACATACA TCTTTGCAGGAGACAAGTTCTGGAGATACAATGAAGTGAAGAAGAAAATGGACCCCGGTTTCCCTAAGCT CATCGCAGACTCCTGGAATGCCATCCCTGATAACCTGGATGCCGTCGTGGACCTGCAGGGTGGTGGTCAT AGCTACTTCTTCAAGGGTGCTTATTACCTGAAGCTGGAGAACCAAAGTCTCAAGAGCGTGAAGTTTGGAA GCATCAAATCAGACTGGCTGGGCTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209857 protein sequence
Red=Cloning site Green=Tags(s) MEARVAWGALAGPLRVLCVLCCLLGRAIAAPSPIIKFPGDVAPKTDKELAVQYLNTFYGCPKESCNLFVL KDTLKKMQKFFGLPQTGDLDQNTIETMRKPRCGNPDVANYNFFPRKPKWDKNQITYRIIGYTPDLDPETV DDAFARALKVWSDVTPLRFSRIHDGEADIMINFGRWEHGDGYPFDGKDGLLAHAFAPGTGVGGDSHFDDD ELWTLGEGQVVRVKYGNADGEYCKFPFLFNGREYSSCTDTGRSDGFLWCSTTYNFEKDGKYGFCPHEALF TMGGNADGQPCKFPFRFQGTSYNSCTTEGRTDGYRWCGTTEDYDRDKKYGFCPETAMSTVGGNSEGAPCV FPFTFLGNKYESCTSAGRNDGKVWCATTTNYDDDRKWGFCPDQGYSLFLVAAHEFGHAMGLEHSQDPGAL MAPIYTYTKNFRLSHDDIKGIQELYGPSPDADTDTGTGPTPTLGPVTPEICKQDIVFDGIAQIRGEIFFF KDRFIWRTVTPRDKPTGPLLVATFWPELPEKIDAVYEAPQEEKAVFFAGNEYWVYSASTLERGYPKPLTS LGLPPDVQQVDAAFNWSKNKKTYIFAGDKFWRYNEVKKKMDPGFPKLIADSWNAIPDNLDAVVDLQGGGH SYFFKGAYYLKLENQSLKSVKFGSIKSDWLGC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008610 |
| ORF Size | 1989 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008610.3 |
| RefSeq Size | 3070 bp |
| RefSeq ORF | 1989 bp |
| Locus ID | 17390 |
| UniProt ID | P33434 |
| MW | 74.1 kDa |
| Gene Summary | This gene encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that are involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded preproprotein undergoes proteolytic processing to generate a mature, zinc-dependent endopeptidase enzyme that hydrolyzes collagens, gelatins, laminin, fibronectin and elastin. Mice lacking the encoded protein exhibit suppressed angiogenesis and attenuated features of human multicentric osteolysis with arthritis including abnormal skeletal and craniofacial development. [provided by RefSeq, Feb 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202668 | Mmp2 (untagged) - Mouse matrix metallopeptidase 2 (Mmp2), (10ug) |
CNY 2,000.00 |
|
| MG209857 | Mmp2 (tGFP-tagged) - Mouse matrix metallopeptidase 2 (Mmp2) |
CNY 6,370.00 |
|
| MR209857L3 | Lenti ORF clone of Mmp2 (Myc-DDK-tagged) - Mouse matrix metallopeptidase 2 (Mmp2) |
CNY 7,336.00 |
|
| MR209857L4 | Lenti ORF clone of Mmp2 (mGFP-tagged) - Mouse matrix metallopeptidase 2 (Mmp2) |
CNY 7,336.00 |
