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Ticam1 (NM_174989) Mouse Tagged Lenti ORF Clone
CAT＃: MR210343L3
- LentiORF®
Lenti ORF clone of Ticam1 (Myc-DDK-tagged) - Mouse toll-like receptor adaptor molecule 1 (Ticam1)
"NM_174989" in other vectors (4)
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CNY 7,700.00
Product images
CNY 600.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AW046014; AW547018; TICAM-1; TRIF |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR210343).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_174989 |
| ORF Size | 2199 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_174989.2 |
| RefSeq Size | 2852 bp |
| RefSeq ORF | 2199 bp |
| Locus ID | 106759 |
| UniProt ID | Q80UF7 |
| Gene Summary | Involved in innate immunity against invading pathogens. Adapter used by TLR3, TLR4 (through TICAM2) and TLR5 to mediate NF-kappa-B and interferon-regulatory factor (IRF) activation, and to induce apoptosis (PubMed:12855817, PubMed:16002681, PubMed:21703541). Ligand binding to these receptors results in TRIF recruitment through its TIR domain (PubMed:12855817, PubMed:16002681, PubMed:21703541). Distinct protein-interaction motifs allow recruitment of the effector proteins TBK1, TRAF6 and RIPK1, which in turn, lead to the activation of transcription factors IRF3 and IRF7, NF-kappa-B and FADD respectively (PubMed:12855817, PubMed:16002681, PubMed:21703541). Phosphorylation by TBK1 on the pLxIS motif leads to recruitment and subsequent activation of the transcription factor IRF3 to induce expression of type I interferon and exert a potent immunity against invading pathogens (By similarity). Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of proinflammatory cytokines (PubMed:21703541).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221188 | Ticam1 (untagged) - Mouse toll-like receptor adaptor molecule 1 (Ticam1), (10ug) |
CNY 9,500.00 |
|
| MG210343 | Ticam1 (tGFP-tagged) - Mouse toll-like receptor adaptor molecule 1 (Ticam1) |
CNY 6,370.00 |
|
| MR210343 | Ticam1 (Myc-DDK-tagged) - Mouse toll-like receptor adaptor molecule 1 (Ticam1) |
CNY 5,368.00 |
|
| MR210343L4 | Lenti ORF clone of Ticam1 (mGFP-tagged) - Mouse toll-like receptor adaptor molecule 1 (Ticam1) |
CNY 7,700.00 |
