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Eif4g2 (NM_001040131) Mouse Tagged ORF Clone
CAT＃: MR211011
- TrueORF®
Eif4g2 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 4, gamma 2 (Eif4g2), transcript variant 2
ORF Plasmid: tGFP
"NM_001040131" in other vectors (4)
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CNY 6,360.00
CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DAP; DAP-5; E130105L11Rik; Na; Nat; Nat1; Natm1; p97 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211011 representing NM_001040131
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC GTGGAGAGTGCGATTGCAGAAGGGGGTGCTTCTCGTTTCAGTGCTTCTTCGGGCGGAGGAGGAAGTAGGG GTGCACCTCAGCACTATCCCAAGACTGCTGGCAACAGCGAGTTCCTGGGGAAAACCCCAGGGCAAAACGC TCAGAAATGGATTCCTGCACGAAGCACTAGACGAGATGACAACTCCGCAGCAAACAACTCCGCAAATGAA AAAGAACGACATGATGCAATCTTCAGGAAAGTAAGAGGCATACTAAATAAGCTTACTCCTGAAAAGTTTG ACAAGCTATGCCTTGAGCTCCTCAATGTGGGTGTAGAGTCTAAACTCATCCTTAAAGGGGTCATACTGCT GATTGTGGACAAAGCCTTAGAAGAGCCAAAGTATAGCTCGCTGTATGCTCAGCTATGTCTGCGATTGGCA GAAGATGCACCAAACTTTGATGGCCCAGCAGCAGAGGGTCAACCAGGACAGAAGCAAAGCACAACATTCA GACGCCTCTTGATTTCCAAATTGCAAGATGAATTTGAAAACCGAACCAGAAATGTTGATGTCTATGATAA GCGTGAAAATCCCCTCCTTCCTGAGGAGGAGGAACAGAGAGCCATTGCTAAGATCAAGATGTTGGGGAAC ATCAAATTCATTGGAGAACTTGGCAAGCTTGATCTTATTCATGAATCTATCCTTCATAAGTGCATCAAAA CACTTTTGGAAAAGAAGAAGAGAGTCCAACTCAAAGATATGGGAGAGGATTTGGAGTGCCTCTGTCAGAT AATGAGGACAGTGGGACCTCGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCAGA ATGTGTTCCTTAATGTTAAGTAAGGAATTGCCAGCCAGGATTCGTTTCCTACTGCAGGATACTGTAGAGT TGCGAGAGCACCATTGGGTTCCTCGCAAGGCTTTTCTTGACAATGGACCAAAGACGATCAATCAAATCCG TCAAGATGCAGTAAAAGATCTAGGAGTGTTTATTCCTGCTCCTATGGCTCAAGGGAGGAATGACTTCTTC CTGGAGGGACCGTTCATGCCGCCAAGGATGAAAATGGATAGGGACCCACTTGGGGGACTTGCTGATATGT TTGGACAAATGCCAGGTAGTGGAATTGGTACTGGTCCAGGAGTTATCCAGGATAGATTTTCACCCACAAT GGGACGTCATCGTTCAAATCAGCTCTTCAATGGCCATGGGGGGCACATCATGCCTCCCACGCAATCGCAG TTTGGAGAGATGGGGGGCAAGTTTATGAAAAGCCAGATTAGTTTGAGGCCTGCTCAGTCGTTTCTAATGA ATAAAAATCAGGTGCCAAAGCTTCAGCCCCAGATAACTATGATTCCTCCCAGTGCACAGCCACCACGCAC TCAAACACCGCCTCTGGGACAGACACCTCAACTTGGTCTCAAAACTAATCCACCACTTATCCAGGAAAAG CCTGCCAAGACTAGCAAAAAGCCACCACCATCAAAGGAAGAACTACTTAAACTGACCGAAGCCGTTGTGA CTGACTATCTGAACAGTGGAAATGCCAACGACGCTGTCAGTGGTGTGAGAGAAATGAGAGCTCCAAAACA CTTTCTTCCTGAGATGCTAAGCAAAGTGATCATCCTGTCACTTGATAGAAGTGATGAAGATAAAGAAAAA GCAAGCTCTTTAATCAGTTTACTCAAACAGGAAGGGATAGCCACAAGTGACAACTTCATGCAGGCTTTCC TGAATGTATTGGAGCAGTGCCCCAAACTGGAGGTTGACATCCCCTTGGTGAAATCTTACTTGGCACAGTT TGCAGCTCGTGCTATAATTTCAGAGTTGGTGAGCATTTCCGAACTAGCTCAACCACTGGAGAGTGGCACC CACTTCCCTCTCTTCTTACTTTGTCTTCAACAATTAGCTAAATTGCAAGACCGAGAGTGGTTAACCGAAC TTTTTCAACAAAGCAAGGTCAATATGCAGAAAATGCTGCCAGAAATTGATCAGAATAAGGATCGAATGTT GGAGATTTTGGAAGGAAAGGGACTGAGTTTCTTATTCCCACTCCTTAAATTGGAGAAGGAACTATTGAAG CAAATTAAGCTGGATCCATCCCCTCAAACTATATATAAATGGATTAAAGATAACATCTCTCCCAAACTTC ATGTAGATAAAGGATTCGTGAACATCTTAATGACCAGCTTCTTACAGTACATTTCTAGTGAAGTAAGCCC ACCCAGCGATGAAACAGATTCTTCCTCTGCTCCTTCCAAAGAGCAGTTAGAGCAGGAAAAACAGCTGCTG CTCTCTTTTAAGCCAGTGATGCAGAAATTTCTTCATGATCATGTGGATCTACAGGTCAGTGCCCTGTATG CTTTGCAGGTGCACTGTTACAACAGCAGCTTCCCAAAAGGCATGTTACTTCGATTTTTTGTTCACTTCTA TGACATGGAAATTATTGAAGAGGAAGCTTTCTTAGCTTGGAAGGAAGACATAACTCAAGAGTTTCCAGGA AAAGGCAAGGCTTTGTTCCAGGTGAATCAGTGGCTAACCTGGCTAGAAACTGCTGAAGAAGAAGAATCAG AGGAAGAAGCTGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211011 representing NM_001040131
Red=Cloning site Green=Tags(s) VESAIAEGGASRFSASSGGGGSRGAPQHYPKTAGNSEFLGKTPGQNAQKWIPARSTRRDDNSAANNSANE KERHDAIFRKVRGILNKLTPEKFDKLCLELLNVGVESKLILKGVILLIVDKALEEPKYSSLYAQLCLRLA EDAPNFDGPAAEGQPGQKQSTTFRRLLISKLQDEFENRTRNVDVYDKRENPLLPEEEEQRAIAKIKMLGN IKFIGELGKLDLIHESILHKCIKTLLEKKKRVQLKDMGEDLECLCQIMRTVGPRLDHERAKSLMDQYFAR MCSLMLSKELPARIRFLLQDTVELREHHWVPRKAFLDNGPKTINQIRQDAVKDLGVFIPAPMAQGRNDFF LEGPFMPPRMKMDRDPLGGLADMFGQMPGSGIGTGPGVIQDRFSPTMGRHRSNQLFNGHGGHIMPPTQSQ FGEMGGKFMKSQISLRPAQSFLMNKNQVPKLQPQITMIPPSAQPPRTQTPPLGQTPQLGLKTNPPLIQEK PAKTSKKPPPSKEELLKLTEAVVTDYLNSGNANDAVSGVREMRAPKHFLPEMLSKVIILSLDRSDEDKEK ASSLISLLKQEGIATSDNFMQAFLNVLEQCPKLEVDIPLVKSYLAQFAARAIISELVSISELAQPLESGT HFPLFLLCLQQLAKLQDREWLTELFQQSKVNMQKMLPEIDQNKDRMLEILEGKGLSFLFPLLKLEKELLK QIKLDPSPQTIYKWIKDNISPKLHVDKGFVNILMTSFLQYISSEVSPPSDETDSSSAPSKEQLEQEKQLL LSFKPVMQKFLHDHVDLQVSALYALQVHCYNSSFPKGMLLRFFVHFYDMEIIEEEAFLAWKEDITQEFPG KGKALFQVNQWLTWLETAEEEESEEEAD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001040131 |
| ORF Size | 2604 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001040131.2, NP_001035221.1 |
| RefSeq Size | 7646 bp |
| RefSeq ORF | 2607 bp |
| Locus ID | 13690 |
| UniProt ID | Q62448 |
| MW | 98.3 kDa |
| Gene Summary | Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G, that contains the binding sites for eIF4A and eIF3; eIF4G in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. Transgene expression of the apolipoprotein B mRNA-editing enzyme (APOBEC-1) causes extensive editing of this mRNA, which could contribute to the potent oncogenesis induced by overexpression of APOBEC-1. In vitro and in vivo studies in human indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. This also appears to be true for mouse. Two alternatively spliced transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222254 | Eif4g2 (untagged) - Mouse eukaryotic translation initiation factor 4, gamma 2 (Eif4g2), transcript variant 2, (10ug) |
CNY 11,120.00 |
|
| MG211011 | Eif4g2 (tGFP-tagged) - Mouse eukaryotic translation initiation factor 4, gamma 2 (Eif4g2), transcript variant 2 |
CNY 7,890.00 |
|
| MR211011L3 | Lenti ORF clone of Eif4g2 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 4, gamma 2 (Eif4g2), transcript variant 2 |
CNY 9,030.00 |
|
| MR211011L4 | Lenti ORF clone of Eif4g2 (mGFP-tagged) - Mouse eukaryotic translation initiation factor 4, gamma 2 (Eif4g2), transcript variant 2 |
CNY 9,030.00 |
