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Sema6a (NM_018744) Mouse Tagged ORF Clone
CAT＃: MR211486
- TrueORF®
Sema6a (Myc-DDK-tagged) - Mouse sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A (Sema6a)
ORF Plasmid: tGFP
"NM_018744" in other vectors (4)
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CNY 10,776.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | A730020P05Rik; AI851735; Sema6A-1; Semaq; VIa |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211486 representing NM_018744
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGCCAGCAGCCTTACTGCTGTGTCTCACACTGCTACACTGCGCTGGGGCTGGTTTCCCAGAAGATT CCGAGCCAATCAGTATTTCGCATGGCAACTATACAAAACAGTATCCGGTGTTTGTGGGCCACAAGCCAGG ACGGAACACCACGCAGAGGCACAGGCTGGACATCCAGATGATCATGATCATGAACAGAACCCTCTACGTT GCTGCTCGAGACCATATTTATACTGTTGATATAGACACATCCCACACAGAAGAAATTTACTGTAGCAAAA AACTGACATGGAAATCTAGACAGGCTGACGTAGACACATGCAGGATGAAGGGGAAACATAAGGATGAATG TCACAACTTCATTAAAGTTCTTCTCAAGAAGAATGATGATACGCTGTTTGTCTGTGGAACCAATGCCTTC AACCCTTCCTGCAGAAACTACAGGGTCGATACCTTGGAAACTTTTGGGGATGAATTTAGCGGAATGGCCA GATGCCCTTATGATGCCAAACATGCCAACATCGCTCTGTTTGCAGATGGAAAACTCTACTCGGCTACAGT GACTGACTTTCTGGCCATTGATGCAGTCATTTACAGGAGCCTCGGAGACAGCCCTACCCTCAGGACTGTC AAGCATGATTCAAAGTGGTTGAAAGAGCCGTACTTTGTCCAAGCCGTGGATTATGGGGACTATATCTACT TCTTCTTCAGAGAAATTGCAGTAGAATACAACACTATGGGGAAGGTTGTTTTCCCTAGGGTGGCTCAGGT CTGTAAGAATGACATGGGAGGGTCTCAGAGAGTCCTGGAGAAGCAGTGGACATCTTTCCTGAAGGCTCGC CTGAACTGCTCGGTGCCTGGAGACTCTCATTTTTATTTCAATATACTCCAGGCAGTTACAGATGTGATTC GCATTAATGGCCGTGATGTTGTCTTGGCAACCTTTTCCACACCTTATAACAGCATCCCAGGTTCTGCAGT CTGTGCCTATGACATGCTTGACATTGCTAATGTTTTCACTGGGAGGTTCAAGGAACAGAAATCACCTGAC TCTACCTGGACACCCGTTCCAGACGAACGAGTCCCTAAGCCCAGGCCAGGCTGTTGTGCTGGATCATCCT CTTTAGAAAAATATGCAACCTCCAATGAGTTTCCCGATGATACCCTGAACTTCATTAAGACGCATCCACT CATGGACGAGGCAGTGCCTTCCATCATCAACAGACCTTGGTTCCTGAGAACAATGGTCAGATACCGCCTG ACCAAAATTGCAGTAGACAACGCTGCCGGGCCATATCAGAATCACACTGTGGTTTTCCTGGGATCAGAAA AGGGAATCATCCTGAAGTTCTTGGCCAGGATAGGAAGCAGTGGTTTCCTAAATGGCAGCCTTTTCCTGGA GGAGATGAATGTTTACAACCCAGAAAAGTGCAGCTATGATGGTGTAGAAGACAAAAGGATCATGGGCATG CAGCTCGACAGAGCGAGTGGCTCACTCTATGTTGCATTCTCTACTTGTGTGATCAAGGTGCCTCTTGGCC GCTGTGAGCGACATGGGAAGTGTAAAAAAACCTGCATCGCCTCCAGAGACCCGTATTGTGGGTGGGTAAG GGAAAGTGGTTCCTGTGCCCATCTGTCACCCCTTAGCAGACTGACATTTGAGCAGGACATTGAGCGTGGC AATACGGACGGCCTAGGAGACTGTCACAATTCCTTCGTGGCACTGAATGGGCACGCCAGTTCCCTCTATC CCAGCACCACTACGTCAGATTCGGCATCCCGAGACGGGTATGAGTCTAGGGGAGGCATGCTGGACTGGAA CGACCTGCTCGAGGCACCTGGCAGCACAGACCCTTTGGGGGCAGTGTCCTCTCATAACCACCAGGACAAG AAGGGAGTGATTCGGGAAAGTTACCTCAAAAGCAACGACCAGCTTGTTCCTGTCACCCTCCTGGCCATTG CAGTCATTCTGGCTTTTGTCATGGGGGCCGTCTTCTCGGGCATCATCGTGTATTGTGTGTGCGATCACCG GCGCAAAGACGTGGCAGTAGTGCAGCGCAAGGAGAAAGAGCTCACTCACTCGCGTCGGGGATCTATGAGC AGTGTCACCAAGCTCAGTGGCCTCTTTGGGGACACCCAGTCCAAGGACCCAAAGCCTGAGGCCATCCTCA CACCACTCATGCACAACGGCAAGCTGGCCACGCCTAGCAACACCGCCAAGATGCTCATCAAGGCTGACCA GCATCACCTAGACCTCACCGCCCTGCCCACCCCAGAGTCCACCCCGACACTGCAGCAGAAACGGAAACCC AACCGCGGCAGTCGCGAGTGGGAGAGGAACCAGAACATCATCAATGCCTGCACCAAGGACATGCCTCCCA TGGGTTCCCCTGTGATTCCCACGGACCTGCCCCTCCGGGCCTCCCCAAGCCACATCCCCAGCGTGGTGGT CCTGCCCATCACGCAGCAGGGCTACCAGCACGAGTACGTAGATCAGCCCAAAATGAGCGAGGTGGTGGCT CAGATGGCACTGGAGGACCAGGCTGCCACCCTGGAGTATAAGACCATCAAAGAGCACCTGAGTAGCAAGA GTCCCAACCATGGGGTGAACCTTGTGGAGAACCTGGACAGCCTGCCCCCTAAAGTTCCACAGCGCGAGGC CTCCCTAGGTCCCCCGGGAACCTCACTGTCACAGACCGGCCTGAGCAAGAGGCTGGAGATGCAACACTCC TCCTCCTATGGGCTCGAATATAAGAGGAGCTACCCCACGAACTCGCTCACAAGAAGCCACCAGACCACCA CTCTCAAAAGAAACAATACTAACTCCTCCAATTCCTCCCACCTCTCCAGGAACCAGAGCTTTGGCCGGGG AGACAACCCACCCCCCGCCCCGCAGCGGGTGGACTCTATCCAGGTGCACAGCTCCCAGCCCTCTGGCCAG GCCGTGACTGTTTCGAGGCAGCCCAGCCTCAATGCCTACAACTCACTGACGAGGTCGGGGCTGAAGCGCA CCCCCTCGCTAAAGCCAGATGTACCCCCCAAACCTTCCTTTGCTCCCCTTTCCACATCCATGAAGCCCAA TGATGCATGTACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211486 representing NM_018744
Red=Cloning site Green=Tags(s) MRPAALLLCLTLLHCAGAGFPEDSEPISISHGNYTKQYPVFVGHKPGRNTTQRHRLDIQMIMIMNRTLYV AARDHIYTVDIDTSHTEEIYCSKKLTWKSRQADVDTCRMKGKHKDECHNFIKVLLKKNDDTLFVCGTNAF NPSCRNYRVDTLETFGDEFSGMARCPYDAKHANIALFADGKLYSATVTDFLAIDAVIYRSLGDSPTLRTV KHDSKWLKEPYFVQAVDYGDYIYFFFREIAVEYNTMGKVVFPRVAQVCKNDMGGSQRVLEKQWTSFLKAR LNCSVPGDSHFYFNILQAVTDVIRINGRDVVLATFSTPYNSIPGSAVCAYDMLDIANVFTGRFKEQKSPD STWTPVPDERVPKPRPGCCAGSSSLEKYATSNEFPDDTLNFIKTHPLMDEAVPSIINRPWFLRTMVRYRL TKIAVDNAAGPYQNHTVVFLGSEKGIILKFLARIGSSGFLNGSLFLEEMNVYNPEKCSYDGVEDKRIMGM QLDRASGSLYVAFSTCVIKVPLGRCERHGKCKKTCIASRDPYCGWVRESGSCAHLSPLSRLTFEQDIERG NTDGLGDCHNSFVALNGHASSLYPSTTTSDSASRDGYESRGGMLDWNDLLEAPGSTDPLGAVSSHNHQDK KGVIRESYLKSNDQLVPVTLLAIAVILAFVMGAVFSGIIVYCVCDHRRKDVAVVQRKEKELTHSRRGSMS SVTKLSGLFGDTQSKDPKPEAILTPLMHNGKLATPSNTAKMLIKADQHHLDLTALPTPESTPTLQQKRKP NRGSREWERNQNIINACTKDMPPMGSPVIPTDLPLRASPSHIPSVVVLPITQQGYQHEYVDQPKMSEVVA QMALEDQAATLEYKTIKEHLSSKSPNHGVNLVENLDSLPPKVPQREASLGPPGTSLSQTGLSKRLEMQHS SSYGLEYKRSYPTNSLTRSHQTTTLKRNNTNSSNSSHLSRNQSFGRGDNPPPAPQRVDSIQVHSSQPSGQ AVTVSRQPSLNAYNSLTRSGLKRTPSLKPDVPPKPSFAPLSTSMKPNDACT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018744 |
| ORF Size | 3096 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018744.2, NP_061214.2 |
| RefSeq Size | 6901 bp |
| RefSeq ORF | 3096 bp |
| Locus ID | 20358 |
| UniProt ID | O35464 |
| MW | 114.4 kDa |
| Gene Summary | Cell surface receptor for PLXNA2 that plays an important role in cell-cell signaling. Required for normal granule cell migration in the developing cerebellum. Promotes reorganization of the actin cytoskeleton and plays an important role in axon guidance in the developing central nervous system. Can act as repulsive axon guidance cue. Has repulsive action towards migrating granular neurons. May play a role in channeling sympathetic axons into the sympathetic chains and controlling the temporal sequence of sympathetic target innervation.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
In vivo epigenetic editing of Sema6a promoter reverses transcallosal dysconnectivity caused by C11orf46/Arl14ep risk gene
,Peter, CJ;Saito, A;Hasegawa, Y;Tanaka, Y;Nagpal, M;Perez, G;Alway, E;Espeso-Gil, S;Fayyad, T;Ratner, C;Dincer, A;Gupta, A;Devi, L;Pappas, JG;Lalonde, FM;Butman, JA;Han, JC;Akbarian, S;Kamiya, A;,
Nat Commun
,PubMed ID 31511512
[SEMA6A]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223265 | Sema6a (untagged) - Mouse sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A (Sema6a), (10ug) |
CNY 10,776.00 |
|
| MG211486 | Sema6a (tGFP-tagged) - Mouse sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A (Sema6a) |
CNY 11,310.00 |
|
| MR211486L3 | Lenti ORF clone of Sema6a (Myc-DDK-tagged) - Mouse sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A (Sema6a) |
CNY 12,160.00 |
|
| MR211486L4 | Lenti ORF clone of Sema6a (mGFP-tagged) - Mouse sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A (Sema6a) |
CNY 12,160.00 |
