Dll4 (NM_019454) Mouse Tagged ORF Clone

CAT＃: MR212151

TrueORF^®

Dll4 (Myc-DDK-tagged) - Mouse delta-like 4 (Drosophila) (Dll4)

Datasheet

SDS

Plasmid Map

"NM_019454" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 5,024.00

CNY 6,840.00

货期*

现货

规格

Cited in 1 publication.

Product images

经常一起买 (4)

PS100001

pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

CNY 5,420.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA592569S

DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

CNY 300.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Delta4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR212151 representing NM_019454 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGCCTGCGTCCCGGAGCGCCTGTCGCTGGGCGCTACTGCTGCTGGCGGTACTGTGGCCGCAGCAGC GCGCTGCGGGCTCCGGCATCTTCCAGCTGCGGCTGCAGGAGTTCGTCAACCAGCGCGGTATGCTGGCCAA TGGGCAGTCCTGCGAACCGGGCTGCCGGACTTTCTTCCGCATTTGCCTTAAGCACTTCCAGGCAACCTTC TCCGAGGGACCCTGCACCTTTGGCAATGTCTCCACGCCGGTATTGGGCACCAACTCCTTCGTCGTCAGGG ACAAGAATAGCGGCAGTGGTCGCAACCCTCTGCAGTTGCCCTTCAATTTCACCTGGCCGGGAACCTTCTC ACTCAACATCCAAGCTTGGCACACACCGGGAGACGACCTGCGGCCAGAGACTTCGCCAGGAAACTCTCTC ATCAGCCAAATCATCATCCAAGGCTCTCTTGCTGTGGGTAAGATTTGGCGAACAGACGAGCAAAATGACA CCCTCACCAGACTGAGCTACTCTTACCGGGTCATCTGCAGTGACAACTACTATGGAGAGAGCTGTTCTCG CCTATGCAAGAAGCGCGATGACCACTTCGGACATTATGAGTGCCAGCCAGATGGCAGCCTGTCCTGCCTG CCGGGCTGGACTGGGAAGTACTGTGACCAGCCTATATGTCTTTCTGGCTGTCATGAGCAGAATGGTTACT GCAGCAAGCCAGATGAGTGCATCTGCCGTCCAGGTTGGCAGGGTCGCCTGTGCAATGAATGTATCCCCCA CAATGGCTGTCGTCATGGCACCTGCAGCATCCCCTGGCAGTGTGCCTGCGATGAGGGATGGGGAGGTCTG TTTTGTGACCAAGATCTCAACTACTGTACTCACCACTCTCCGTGCAAGAATGGATCAACGTGTTCCAACA GTGGGCCAAAGGGTTATACCTGCACCTGTCTCCCAGGCTACACTGGTGAGCACTGTGAGCTGGGACTCAG CAAGTGTGCCAGCAACCCCTGTCGAAATGGTGGCAGCTGTAAGGACCAGGAGAATAGCTACCACTGCCTG TGTCCCCCAGGCTACTATGGCCAGCACTGTGAGCATAGTACCTTGACCTGTGCGGACTCACCCTGCTTCA ATGGGGGCTCTTGCCGGGAGCGCAACCAGGGGTCCAGTTATGCCTGCGAATGCCCCCCCAACTTTACCGG CTCTAACTGTGAGAAGAAAGTAGACAGGTGTACCAGCAACCCGTGTGCCAATGGAGGCCAGTGCCTGAAC AGAGGTCCAAGCCGAACCTGCCGCTGCCGGCCTGGATTCACAGGCACCCACTGTGAACTGCACATCAGCG ATTGTGCCCGAAGTCCCTGTGCCCACGGGGGCACTTGCCACGATCTGGAGAATGGGCCTGTGTGCACCTG CCCCGCTGGCTTCTCTGGCAGGCGCTGCGAGGTGCGGATAACCCACGATGCCTGTGCCTCCGGACCCTGC TTCAATGGGGCCACCTGCTACACTGGCCTCTCCCCAAACAACTTCGTCTGCAACTGTCCTTATGGCTTTG TGGGCAGCCGCTGCGAGTTTCCCGTGGGCTTGCCACCCAGCTTCCCCTGGGTAGCTGTCTCGCTGGGCGT GGGGCTAGTGGTACTGCTGGTGCTGCTGGTCATGGTGGTAGTGGCTGTGCGGCAGCTGCGGCTTCGGAGG CCCGATGACGAGAGCAGGGAAGCCATGAACAATCTGTCAGACTTCCAGAAGGACAACCTAATCCCTGCCG CCCAGCTCAAAAACACAAACCAGAAGAAGGAGCTGGAAGTGGACTGTGGTCTGGACAAGTCCAATTGTGG CAAACTGCAGAACCACACATTGGACTACAATCTAGCCCCGGGACTCCTAGGACGGGGCAGCATGCCTGGG AAGTATCCTCACAGTGACAAGAGCTTAGGAGAGAAGGTGCCACTTCGGTTACACAGTGAGAAGCCAGAGT GTCGAATATCAGCCATTTGCTCTCCCAGGGACTCTATGTACCAATCAGTGTGTTTGATATCAGAAGAGAG GAACGAGTGTGTGATTGCCACAGAGGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR212151 representing NM_019454 Red=Cloning site Green=Tags(s) MTPASRSACRWALLLLAVLWPQQRAAGSGIFQLRLQEFVNQRGMLANGQSCEPGCRTFFRICLKHFQATF SEGPCTFGNVSTPVLGTNSFVVRDKNSGSGRNPLQLPFNFTWPGTFSLNIQAWHTPGDDLRPETSPGNSL ISQIIIQGSLAVGKIWRTDEQNDTLTRLSYSYRVICSDNYYGESCSRLCKKRDDHFGHYECQPDGSLSCL PGWTGKYCDQPICLSGCHEQNGYCSKPDECICRPGWQGRLCNECIPHNGCRHGTCSIPWQCACDEGWGGL FCDQDLNYCTHHSPCKNGSTCSNSGPKGYTCTCLPGYTGEHCELGLSKCASNPCRNGGSCKDQENSYHCL CPPGYYGQHCEHSTLTCADSPCFNGGSCRERNQGSSYACECPPNFTGSNCEKKVDRCTSNPCANGGQCLN RGPSRTCRCRPGFTGTHCELHISDCARSPCAHGGTCHDLENGPVCTCPAGFSGRRCEVRITHDACASGPC FNGATCYTGLSPNNFVCNCPYGFVGSRCEFPVGLPPSFPWVAVSLGVGLVVLLVLLVMVVVAVRQLRLRR PDDESREAMNNLSDFQKDNLIPAAQLKNTNQKKELEVDCGLDKSNCGKLQNHTLDYNLAPGLLGRGSMPG KYPHSDKSLGEKVPLRLHSEKPECRISAICSPRDSMYQSVCLISEERNECVIATEV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_019454
ORF Size	2058 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_019454.2, NP_062327.1
RefSeq Size	3426 bp
RefSeq ORF	2061 bp
Locus ID	54485
UniProt ID	Q9JI71
MW	75.4 kDa
Gene Summary	Involved in the Notch signaling pathway as Notch ligand (PubMed:11134954). Activates NOTCH1 and NOTCH4. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting (By similarity). Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types (PubMed:22323600). During spinal cord neurogenesis, inhibits V2a interneuron fate (By similarity).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

The use of this cDNA Clones has been cited in the following citations:
EHD2 modulates Dll4 endocytosis during blood vessel development, et al. EHD2 modulates Dll4 endocytosis during blood vessel development ,null, Microcirculation (New York, N.y. : 1994) ,PubMed ID 34820962 [Dll4]

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
MC204452	Dll4 (untagged) - Mouse delta-like 4 (Drosophila) (Dll4), (10ug)	10 ug 产品选项验证失败所有选项都是必需的	CNY 2,000.00
MR212151L3	Lenti ORF clone of Dll4 (Myc-DDK-tagged) - Mouse delta-like 4 (Drosophila) (Dll4)	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,424.00 CNY 8,740.00
MR212151L4	Lenti ORF clone of Dll4 (mGFP-tagged) - Mouse delta-like 4 (Drosophila) (Dll4)	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,424.00 CNY 8,740.00

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