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Ambra1 (NM_172669) Mouse Tagged ORF Clone
CAT＃: MR215908
- TrueORF®
Ambra1 (Myc-DDK-tagged) - Mouse autophagy/beclin 1 regulator 1 (Ambra1), transcript variant 1
ORF Plasmid: tGFP
"NM_172669" in other vectors (6)
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CNY 13,576.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2310079H06Rik; A130023A14; AA474864; AV021921; D030051N19Rik; mKIAA1736 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR215908 representing NM_172669
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGTTGTCCCAGAGAAGAATGCTGTACGAATCCTCTGGGGCCGAGAACGGGGCACTCGTGCCATGG GTGCCCAGCGGCTTCTGCAGGAGCTGGTGGAGGATAAGACTCGATGGATGAAATGGGAGGGCAAGAGAGT AGAACTGCCTGATAGTCCACGCTCGACCTTCTTACTGGCCTTCAGCCCAGACAGGACTCTCTTGGCATCC ACACATGTGAACCATAACATCTATATTACAGAGGTTAAGACTGGCAAGTGTGTTCACTCTCTGATCGGAC ACCGCCGTACTCCATGGTGTGTCACTTTTCACCCCACCATCTCAGGCCTTATTGCTTCTGGTTGCCTAGA TGGGGAAGTTAGGATTTGGGATTTACATGGGGGCAGTGAAAGCTGGTTCACAGACAGCAACAATGCCATA GCCTCCTTGGCTTTCCACCCTACGGCTCAGCTTCTACTAATTGCCACTGCAAATGAGATCCATTTCTGGG ATTGGAGTCGTCGGGAGCCCTTTGCTGTGGTGAAAACAGCCAGTGAGATGGAGCGTGTCCGCCTGGTGAG ATTTGATCCACTTGGACACTACTTACTTACAGCTATTGTTAACCCTTCTAATCAGCAGGGTGATGACGAA CCAGAAATCCCTATAGATGGAACAGAGTTATCTCATTACCGTCAACGTGCCCTCCTGCAATCACAGCCAG TTCGCCGGACACCTCTCCTCCACAATTTCCTGCACATGCTGTCCTCCCGTTCCTCTGGCATCCAGGTGGG AGAGCAAAGCACAGTACAAGATTCTGCTACCCCCTCACCCCCACCGCCTCCCCCTCAGCCCTCCACGGAG CGTCCCAGGACTTCCGCTTACATCAGACTCCGACAGCGGGTCAGTTACCCCACCACAGTTGAGTGCTGCC AGCACCCTGGGATCCTGTGCCTTTGCAGCCGCTGTGCTGGCACTCGAGTTCCTTCCCTCTTGCCACACCA GGACAGTGTCCCCCCTGCTTCTGCCAGAGCTACTACCCCTTCCTTTTCTTTTGTACAGACCGAGCCCTTC CATCCCCCGGAGCAGGCTTCATCAACGCAGCAGGACCAGGGCCTCCTGAACCGGCCGTCTGCCTTCAGTA CAGTCCAGAGCAGCACTGCGGGCAACACGCTCCGCAACCTCAGTCTGGGTCCTACCCGGCGCTCTTTGGG GGGCCCTTTGTCTAGCCACCCTTCTAGGTATCACAGAGAACTAGCCCCTGGGCTGACAGGTTCTGAGTGG ACCCGGACAGTACTCACTCTGAACTCTCGTTCTGAGGTAGAATCTATGCCCCCACCGAGGACCAGTGCCT CCTCAGTGAGTTTGCTTTCTGTACTGAGACAGCAGGAAGGTGGCTCTCAGGCATCTGTGTATACTTCAGC CACAGAAGGGAGGGGTTTTCCATCATCAGGGTTGGCAACTGAGTCAGATGGAGGGAATGGTTCCAGCCAA AACAACTCAGGCAGCATTCGCCATGAGCTTCAATGTGACCTGAGACGCTTCTTTTTGGAGTATGACAGGC TGCAGGAGCTGGATCAGAGCCTGAGTGGGGAAACTCCCCAGACCCAACAGGCCCAGGAAATGCTCAACAA TAATATTGAATCTGAGAGACCAGGTCCTTCCCACCTGCCTACCCCACACAGCAGTGAGAACAACTCCAAC CTGTCTCGGGGCCACCTTAATCGATGCCGTGCTTGCCATAATCTTCTAACCTTCAACAATGATACCCTTC GTTGGGAAAGAACCACACCTAACTACTCATCTGGTGAGGCTAGCTCTTCCTGGCACGTCTCCACTACCTT TGAGGGCATGCCACCAAGTGGCAACCAGCTACCACCTCTTGAGCGGACTGAGGGCCAAATGCCTAGCTCC AGCAGGCTGGAGTTGAGCAGCTCTGCTAGTTCACAAGAGGAGAGGACTGTGGGGGTGGCCTTTAACCAGG AGACAGGCCACTGGGAACGCATTTATACCCAGTCCAGCAGATCTGGAACTGTATCACAGGAGGCCTTACA TCAAGATATGCCTGAAGAAAGCTCTGAGGAAGACTCCCTCAGGAGGAGGCTGCTGGAGTCTTCCCTCATT TCATTATCCCGTTATGATGGAGCAGGATCCAGAGAGCACCCAATTTACCCAGACCCAGCGAGATTATCTC CTGCTGCATACTACGCCCAGAGGATGATCCAGTATCTCTCAAGGAGAGACAGTATTCGCCAGCGCTCCAT GCGCTACCAACAGAATCGGCTTCGTTCTTCCACCTCCTCTTCTTCCTCAGACAACCAGGGTCCATCAGTA GAGGGAACCGACTTGGAATTTGAGGACTTTGAGGACAATGGTGACAGATCTAGGCACCGAGCTCCCCGAA ATGCCCGAATGTCTGCACCTTCACTTGGACGATTTGTTCCAAGGCGTTTTTTGCTGCCTGAGTACTTGCC TTATGCTGGGATTTTTCATGAACGTGGACAGCCTGGCTTGGCTACTCATTCTTCTGTTAACAGGGTCCTG GCAGGGGCAGTAATTGGAGATGGACAGTCTGCTGTGGCCAGTAACATTGCCAATACTACCTACAGGCTCC AGTGGTGGGACTTCACTAAGTTTGACCTTCCAGAAATCAGTAATGCCTCTGTGAATGTCCTGGTACAGAA TTGCAAGATCTACAATGATGCCAGCTGTGACATTTCTGCAGATGGTCAGCTCCTGGCAGCCTTCATCCCG AGCAGCCAGAGGGGCTTTCCTGATGAAGGCATCCTGGCAGTGTATTCCCTGGCCCCCCATAACTTGGGGG AAATGCTCTACACCAAGCGATTTGGTCCCAATGCCATTTCTGTAAGCCTGTCCCCGATGGGCCGATACGT TATGGTGGGCTTGGCCTCACGAAGGATCCTACTGCACCCCTCCACAGAGCACATGGTAGCCCAGGTCTTC AGGCTGCAACAGGCCCATGGTGGTGAGACTTCCATGAGGAGAGTTTTCAATGTCCTTTACCCCATGCCTG CAGACCAACGGAGACACGTCAGCATCAACTCTGCCCGTTGGCTACCCGAGCCAGGGCTTGGTTTGGCCTA TGGCACCAACAAAGGAGACTTGGTGATCTGCCGACCAGAGGCCTTAAACTCTGGCATCGAGTACTACTGG GACCAGCTAAGTGAAACAGTCTTCACTGTCCACTCAAGTAGCAGGAGCAGCGAGCGGCCTGGAACCAGCA GAGCCACGTGGCGGACAGACAGAGACATGGGTTTGATGAATGCAATTGGGCTGCAGCCTCGGAACCCAAC CACCTCTGTGACATCTCAGGGCACCCAGACTCTGGCCCTCCAGTTGCAGAACGCTGAAACACAGACTGAG AGGGAGGAAGAGGAACCAGGGGCAGCATCTTCAGGTCCTGGTGAAGGCGAGGGCTCAGAATATGGTGGCA GTGGGGAAGATGCCCTCAGCAGGATCCAGAGGCTGATGGCCGAGGGTGGCATGACAGCTGTGGTACAACG GGAACAGAGCACCACCATGGCCTCCATGGGTGGCTTTGGCAACAACATCATCGTTAGCCACCGCATTCAC CGCAGCTCCCAGACAGGCACAGAGTCAGGGGCTGCTCGTACCTCCTCACCCCAGCCCTCCACCTCTCGAG GACTGCCATCAGAACCTGGGCAACTGGCAGAGCGAGCGCTAAGCCCCCGGACAGCCTCCTGGGACCAGCC TAGTACCTCTGGGAGGGAACTACCCCAGCCAGCCCTGTCCTCTTCCTCCCCTGTCCCCATTCCAGTCCCC CTTGCCAGCAATGAGGGACCAACCATGCACTGCAATGTGACCAATAACAGTCACCTTCCTGAGGGTGATG GTAGCAACAGGGGGGAAGCTGCAGGCCCTAGTGGAGAGCCACAGAACCGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR215908 representing NM_172669
Red=Cloning site Green=Tags(s) MKVVPEKNAVRILWGRERGTRAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLAS THVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAI ASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDE PEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDSATPSPPPPPPQPSTE RPRTSAYIRLRQRVSYPTTVECCQHPGILCLCSRCAGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPF HPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHRELAPGLTGSEW TRTVLTLNSRSEVESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPSSGLATESDGGNGSSQ NNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGETPQTQQAQEMLNNNIESERPGPSHLPTPHSSENNSN LSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWHVSTTFEGMPPSGNQLPPLERTEGQMPSS SRLELSSSASSQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRRLLESSLI SLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSV EGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVL AGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIP SSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVF RLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGIEYYW DQLSETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTE REEEEPGAASSGPGEGEGSEYGGSGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIH RSSQTGTESGAARTSSPQPSTSRGLPSEPGQLAERALSPRTASWDQPSTSGRELPQPALSSSSPVPIPVP LASNEGPTMHCNVTNNSHLPEGDGSNRGEAAGPSGEPQNR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_172669 |
| ORF Size | 3900 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_172669.3, NP_766257.3 |
| RefSeq Size | 5329 bp |
| RefSeq ORF | 3903 bp |
| Locus ID | 228361 |
| UniProt ID | A2AH22 |
| MW | 142.9 kDa |
| Gene Summary | Regulates autophagy and development of the nervous system. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224192 | Ambra1 (untagged) - Mouse autophagy/beclin 1 regulator 1 (Ambra1), transcript variant 1, (10ug) |
CNY 13,584.00 |
|
| MG215908 | Ambra1 (tGFP-tagged) - Mouse autophagy/beclin 1 regulator 1 (Ambra1) transcript variant 1, (10ug) |
CNY 14,350.00 |
|
| MR215908L1 | Lenti ORF clone of Ambra1 (Myc-DDK-tagged) - Mouse autophagy/beclin 1 regulator 1 (Ambra1), transcript variant 1 |
CNY 14,920.00 |
|
| MR215908L2 | Lenti ORF clone of Ambra1 (mGFP-tagged) - Mouse autophagy/beclin 1 regulator 1 (Ambra1), transcript variant 1 |
CNY 15,976.00 |
|
| MR215908L3 | Lenti ORF clone of Ambra1 (Myc-DDK-tagged) - Mouse autophagy/beclin 1 regulator 1 (Ambra1), transcript variant 1 |
CNY 14,920.00 |
|
| MR215908L4 | Lenti ORF clone of Ambra1 (mGFP-tagged) - Mouse autophagy/beclin 1 regulator 1 (Ambra1), transcript variant 1 |
CNY 14,920.00 |
