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Gpat2 (NM_001081089) Mouse Tagged ORF Clone
CAT＃: MR218359
- TrueORF®
Gpat2 (Myc-DDK-tagged) - Mouse glycerol-3-phosphate acyltransferase 2, mitochondrial (Gpat2), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_001081089" in other vectors (4)
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CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | A530057A03Rik; Gm116; xGPAT1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR218359 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGAAATCCAACCCCCAAACCCAGCAGAGGAGTAACCACAATGGTCAAGAGACCAGCCTTTGGTCCT CAAGCTTTGGGATGAAGATGGAGGCCATCACTCCATTCCTGGGAAAGTATCGCCCCTTTATGGGCCGCTG CTGCCAGACCTGTACTCCTAAGAGCTGGGAGTCCCTCTTCCACAGAAGCATAATGGACCTGGGCTTCTGC AACGTGATCCTTGTGAAGGAGGAGAACACCAGGTTCCGGGGCTGGCTGGTTCGCAGGCTCTGCTATTTCC TGTGGTCCCTGGAGCAGCACATACCCACCAGTTTTGATGCCTCCCAGAAGATCATGGAAAACACTGGGGT GCAGAACCTCCTCTCGGGGAGGGTTCCGGGAGCGGCTGGAGAAGGCCAGGCGCCTGAATTAGTGAAGAAA GAGGTACAGCGTATCCTGAGCCACATTCAGACCACACCCCGCCCCTTCTTGCTCAGGCTGTTCAGCTGGG CACTGCTGTGGTTCCTGAACCGCCTCTTCCTGAATGTACAACTTCACAAGGGACAGATGAAGATGGTTCA GAAGGCTGTGCAGGAGGGCTCTCCACTTGTCTTCCTTTCTACACACAAGTCCCTCTTGGATGGATTCCTG CTGCCTTTTGTACTGTTCTCCCAAGGCCTCGGTGTGGTCCGTGTGGCTTTAGACTCCCGCACATGTTCCC CTGCTCTCAGAGCTCTATTGAGGAAACTTGGGGGACTTTTCCTGCCCCCAGAGGTCAACCTCTCCTTGGA CAACTCAGAGGGCATCCTTGCAAGGGCTGTGGTCCATGCAACTGTGGAGGAGCTGCTTACTAGTGGTCAG CCTCTGCTCATCTTCCTGGAAGAGCCCCCTGGGTCCCCAGGGCCCAGGCTTTCTGCCCTGGGCCAGGCCT GGCTAGGAGTGGTAATCCAGGCGGTCCAGGCAGGCATTATCTCAGATGCCACACTGGTACCAGTTGCCAT TGCCTATGACCTGGTTCCAGATGCACCATGTAACATGAACCATGATTTGGCTCCCCTGGGGCTATGGACA GGAGCCTTGGCTGTCTTTCGGAGACTGTGTAACTGCTGGGGCTGCAACCGTAGAGTCTGTGTCCGGGTGC ACCTTGCCCAGCCATTTTCCCTCCAGGAATACACCATCAATGCTAGAAGTTGCTGGGATAGCAGGCAGAC CCTGGAACACTTGTTGCAGCCCATTGTGCTAGGTGAATGTAGTGTTGTTCCGGACACTGAGAAGGAGCAG GAGTGGACCCCACCAACTGGCCTCCTCCTGGCCCTTAAAGAGGAGGACCAGCTCCTGGTCAGAAGACTGA GCCGGCATGTTCTGAGTGCCAGTGTGGCCAGCTCCGCAGTGATGAGCACAGCCATCATGGCCACACTGCT TTTGCTGAAGCACCAAAAGGGTGTGGTCCTGTCACAGCTCCTGGGAGAATTCTCCTGGCTGACAGAGGAG ACACTGCTCCGAGGTTTTGATGTGGGCTTCTCAGGGCAGCTGCGGTGCCTGGCCCAACATACACTGAGCC TATTGAGGGCACATGTGGTTCTGCTGCGTGTCCACCAAGGGGACTTGGTGGTGGTACCTCGGCCTGGCCC AGGCCTCACACACCTGGCACGCTTGAGTATGGAATTGCTGCCAACCTTCCTGAGTGAGGCTGTGGGTGCC TGTGCAGTGCGGGGGTTGCTGGCAGGCAGAGTACCACCTGAGGGGCCCTGGGAGCTGCAGGGCATAGAGC TACTGAGTCAGAATGAACTGTACCGCCAGATCCTACTGCTGCTGCACCTGCTACCTCAAGACCTACTGCT GCCCCAGCCCTGCCAGTCTTCCTACTGCTACTGTCAGGAAGTGCTAGACCGGCTCATCCAGTGTGGACTC CTAGTTGCTGAGGAGACTCCAGGCTCCCGGCCAGCCTGTGACACAGGAAGACAGCATCTGAGTGCAAAGC TGCTGTGGAAGCCGAGTGGGGACTTTACTGACAGTGAGAGTGATGACTTTGAGGAGCCAGGAGGCCGCTG CTTCAGGCTTAGCCAGCAATCCCGCTGCCCTGACTTCTTCCTCTTCCTCTGCCGCTTGCTCAGTCCCATA CTCAAGGCCTTCGCACAGGCGGCCACCTTCCTCCACTTAGGGCAGCTGCCAGATTCAGAGGTGGCCTACT CAGAGAAGTTGTTCCAGTTTTTACAGGCCTGTGCCCAAGAAGAAGGGATCTTTGAGTGTGCAGACCCAAA CCTTGCTATCAGTGCAGTCTGGACGTTCAAAGATCTGGGGGTACTTCAGGAGATGCCTAGCCCTACAGGA CCCCAGCTCCACCTGTCCCCTACATTTGCCACCCGGGACAATCAGGACAAGCTGGAACAATTTATCCGAC AGTTCATCTGCAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR218359 protein sequence
Red=Cloning site Green=Tags(s) MLKSNPQTQQRSNHNGQETSLWSSSFGMKMEAITPFLGKYRPFMGRCCQTCTPKSWESLFHRSIMDLGFC NVILVKEENTRFRGWLVRRLCYFLWSLEQHIPTSFDASQKIMENTGVQNLLSGRVPGAAGEGQAPELVKK EVQRILSHIQTTPRPFLLRLFSWALLWFLNRLFLNVQLHKGQMKMVQKAVQEGSPLVFLSTHKSLLDGFL LPFVLFSQGLGVVRVALDSRTCSPALRALLRKLGGLFLPPEVNLSLDNSEGILARAVVHATVEELLTSGQ PLLIFLEEPPGSPGPRLSALGQAWLGVVIQAVQAGIISDATLVPVAIAYDLVPDAPCNMNHDLAPLGLWT GALAVFRRLCNCWGCNRRVCVRVHLAQPFSLQEYTINARSCWDSRQTLEHLLQPIVLGECSVVPDTEKEQ EWTPPTGLLLALKEEDQLLVRRLSRHVLSASVASSAVMSTAIMATLLLLKHQKGVVLSQLLGEFSWLTEE TLLRGFDVGFSGQLRCLAQHTLSLLRAHVVLLRVHQGDLVVVPRPGPGLTHLARLSMELLPTFLSEAVGA CAVRGLLAGRVPPEGPWELQGIELLSQNELYRQILLLLHLLPQDLLLPQPCQSSYCYCQEVLDRLIQCGL LVAEETPGSRPACDTGRQHLSAKLLWKPSGDFTDSESDDFEEPGGRCFRLSQQSRCPDFFLFLCRLLSPI LKAFAQAATFLHLGQLPDSEVAYSEKLFQFLQACAQEEGIFECADPNLAISAVWTFKDLGVLQEMPSPTG PQLHLSPTFATRDNQDKLEQFIRQFICS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001081089 |
| ORF Size | 2397 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001081089.1, NM_001081089.2, NP_001074558.1, NP_001074558.2 |
| RefSeq Size | 2636 bp |
| RefSeq ORF | 2406 bp |
| Locus ID | 215456 |
| UniProt ID | Q14DK4 |
| MW | 88.8 kDa |
| Gene Summary | Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis (PubMed:17013544, PubMed:17689486). Required for primary processing step during piRNA biosynthesis (PubMed:23611983). Molecular mechanisms by which it promotes piRNA biosynthesis are unclear and do not involve its acyltransferase activity (PubMed:23611983).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221731 | Gpat2 (untagged) - Mouse glycerol-3-phosphate acyltransferase 2, mitochondrial (Gpat2), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 10,260.00 |
|
| MG218359 | Gpat2 (tGFP-tagged) - Mouse glycerol-3-phosphate acyltransferase 2 mitochondrial (Gpat2) nuclear gene encoding mitochondrial protein, (10ug) |
CNY 6,370.00 |
|
| MR218359L3 | Lenti ORF clone of Gpat2 (Myc-DDK-tagged) - Mouse glycerol-3-phosphate acyltransferase 2, mitochondrial (Gpat2), nuclear gene encoding mitochondrial protein |
CNY 7,700.00 |
|
| MR218359L4 | Lenti ORF clone of Gpat2 (mGFP-tagged) - Mouse glycerol-3-phosphate acyltransferase 2, mitochondrial (Gpat2), nuclear gene encoding mitochondrial protein |
CNY 7,700.00 |
