Pde4dip (NM_001110163) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	4732458A06Rik; 9430063L05Rik; C87016; D3Bwg1078e; D130016K21Rik; mKIAA0454; Usmg4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR218661 representing NM_001110163 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTAATGGATATCGCACTCTGTCCCAGCACCTCAATGACTTGAAGAAGGAGAACTTCAGCCTCAAGC TGCGCATCTACTTCCTGGAGGAGCGCATGCAACAGAAGTATGAAGTCAGCCGGGAGGATGTCTACAAGCG GAACATCGAGCTGAAGGTTGAAGTGGAGAGCCTGAAACGAGAACTCCAGGACAGGAAACAGCATCTAGAT AAAACATGGGCCGATGCAGAGGATCTCAACAGCCAGAATGAGGCAGAGCTCCGGCGCCAGGTTGAAGAAC GGCAGCAGGAGACAGAACACGTTTATGAGCTCCTAGGGAACAAGATCCAGCTGCTGCAGGAGGAACCCAG GCTAGCAAAGAATGAAGCCACAGAGATGGAGACTCTGGTGGAGGCAGAGAAGAGGTGCAATCTGGAGCTC TCAGAGAGGTGGACGAATGCTGCCAAGAACAGGGAAGATGCAGCAGGAGACCAGGAGAAGCCTGACCAAT ATTCTGAGGCACTGGCTCAGAGGGACAGGAGAATTGAAGAGCTGAGGCAGAGCTTGGCTGCTCAGGAGGG GCTTGTGGAACAGCTGTCTCAAGAGAAACGACAACTGTTACATCTGCTGGAGGAGCCAGCGAGCATGGAA GTGCAGCCCGTGCCTAAAGGGTTACCCACGCAACAGAAGCCGGACTTGCATGAGACCCCTACAACCCAGC CACCTGTGTCTGAGTCCCACCTGGCAGAACTCCAGGACAAAATCCAGCAAACAGAGGCCACCAACAAGAT TCTTCAAGAGAAACTGAATGACCTGAGCTGTGAGCTGAAATCTGCACAGGAGTCATCTCAGAAGCAAGAT ACGACAATCCAGAGCCTCAAGGAAATGCTTAAAAGCAGGGAAAGTGAGACTGAGGAGCTGTACCAGGTGA TCGAAGGACAAAATGACACAATGGCAAAGCTTCGGGAAATGCTGCACCAGAGCCAGCTCGGACAGCTCCA CAGCTCAGAGGGCATTGCCCCTGCTCAGCAACAGGTAGCCCTGCTTGACCTTCAGAGCGCTCTATTCTGC AGCCAGCTTGAAATACAGAGGCTCCAGAGGCTGGTCCGGCAGAAAGAACGCCAGCTGGCGGATGGCAAGC GATGTGTGCAGTTAGTGGAGGCTGCAGCCCAGGAGAGAGAGCACCAGAAGGAAGCTGCTTGGAAACATAA CCAGGAATTACGAAAGGCTTTACAGCACCTCCAAGGAGAACTGCACAGCAAGAGCCAGCAGCTCCATGTT CTGGAGGCGGAAAAATACAATGAGATTCGAACCCAGGGACAAAACATCCAACACCTAAGTCACAGTCTGA GTCACAAAGAGCAGCTAATTCAGGAACTCCAGGAGCTCCTACAGTATCGTGACAACGCAGACAAAACTCT AGACACAAACGAAGTGTTTCTTGAGAAATTGCGGCAACGAATACAAGACCGAGCTGTTGCTCTAGAGCGG GTCATAGATGAGAAGTTCTCTGCTCTAGAAGAAAAGGACAAGGAACTGCGGCAGCTTCGCCTAGCTGTGA GGGACCGAGACCATGACTTAGAGAGACTGCGTTGTGTCCTGTCCGCCAATGAAGCTACCATGCAGAGCAT GGAGAGTCTCCTGAGGGCCAGAGGCCTGGAAGTGGAGCAGTTAACTGCCACCTGCCAAAACCTCCAGTGG CTGAAAGAAGAACTGGAAACCAAATTTGGCCATTGGCAGAAGGAACAGGAGAGCATCATTCAGCAATTGC AGACATCTCTACACGACAGGAACAAAGAAGTAGAGGATCTCAGCGCAACTCTGCTCTGTAAACTTGGACC GGGTCAGAGTGAAGTAGCTGAGGAACTGTGCCAGCGCTTGCAGCGAAAGGAACGGATGCTGCAGGACCTT CTGAGCGATCGGAACAAACAAGCCGTGGAGCACGAGATGGAGATCCAGGGGCTGCTCCAGTCGATGGGCA CCAGGGAGCAGGAAAGACAGGCTGCTGCAGAAAAAATGGTCCAGGCCTTCATGGAAAGGAACTCAGAACT GCAGGCCCTGCGCCAGTATTTAGGGGGGAAGGAACTAATGACATCGTCTCAGACGTTCATCTCTAACCAG CCAGCTGGAGTGACGTCCATCGGGCCTCACCACGGAGAGCAAACCGATCAAGGTTCTATGCAGATGCCCT CTCGAGATGATAGCACCTCACTGACTGCTAGAGAGGAGGCCAGCATACCCCGGTCCACATTAGGAGACTC GGACACAGTTGCAGGGCTGGAGAAAGAACTGAGCAACGCCAAGGAGGAGCTCGAGCTCATGGCCAAAAAA GAAAGAGAAAGCCAGATGGAACTGTCTGCCCTGCAGTCCATGATGGCCATGCAAGAGGAAGAGCTGCAGG TGCAGGCTGCTGACTTGGAGTCCCTGACCAGGAATGTGCAGATAAAAGAAGATCTCATAAAGGACCTGCA GATGCAACTGGTCGACCCTGAAGATATACCAGCCATGGAGCGTCTTACCCAAGAGGTCTTACTTCTTCGG GAAAAAGTTGCTTCCGTGGAACCCCAGGGTCAGGAAGTATCAGGGAACAAGAGACAGCAGTTGCTGCTGA TGTTAGAAGGACTAGTGGATGAACGGAGTCGGCTCAACGAGGCCCTGCAAGCTGAGAGGCAACTCTACAG CAGCCTGGTCAAGTTCCATGCCCAGCCAGAGAACTCTGAGAGAGACGGAACTCTGCAGGTGGAACTGGAA GGGGCCCAGGTGTTACGCACTCGACTAGAAGAAGTTCTTGGAAGAAGCCTGGAGCGTTTAAGCAGGCTGG AGAGCCTGGCCGCCATTGGAGGTGCTACTGCAGGCAATGAGACTGAAGATACAAGCACGGAGTTCACAGA CAGCATTGAGGAGGAGGCTGCACACACCAGCCACCAGCAACTCATCAAGGTGGCTTTGGAGAAAAGCCTG GCAACCATGGAGACCCAGAACATATGTCTTCAGCCCCCTTCCCCAGTAGGAGAGGACAGTAACAGGTGTC TTCAGGAGGAAATGCTCCACCTGAGGGCTGAAATCCACCAGCACTTAGAGGAGAAGAGAAAAGCTGAGGT GGAACTCAAGGAGCTAAAGGCTCAAATTGAGGAAGCAGGATTCTCCTCTGTGTCCCACATCAGGAACACC ATGCTGAGCCTTTGCCTTGAGAACGCAGAGCTGAAGGAGCAGATGGGAGAAGCAATGTCTGATGGATGGG AGGTCGAGGAGGACAAGGAGAAGGGCGAGGTGATGCTGGAGACAGTGGTCGCCAAAGGGTGTCTGAATGA GAACAGCCTTCAGGCTGAGTTCAGGAAAGTCCAGGGGAAACTCAAGAGTGCCTACAACATCATCAACCTC CTCAAAGAGCAGCTGCTCCTGAGGAGCTCGGAAGGGAACAGTAAAGAGATGCCAGAGCTCCTTGTGCGCC TGGCCAGGGAGGTGGACAGAATGAACACGGGTCTGCCTTCCCTGGGGAAGCATCAGCACCAAGAGCAGGA GAATACGACCACCGCAAGGCCTGGCTCGAGACCCCAGAGCCTCCCGCTTGGGGCAGCCCTCTCAGTGGAT GGCTACCAACTGGAGAACAAGTCTCAGGCCCAAGACTCTGGACACCAGCCAGAATTTAGCCTCCCGGGCT CCACCAAACACCTGCGTTCCCAGCTGGCTCAGTGCAGACAACGATACCAAGATCTCCAGGAGAAGTTGCT CATCTCAGAAGCTACCGTGTTTGCCCAGGCAAACCAGCTGGAGAAGTACAGAGCCGTATTCAGTGAATCC CTGGTGAAACAGGACAGCAAGCAGATCCAGGTGGACCTTCAAGACCTGGGCTACGAGACTTGTGGCCGAA GTGAGAATGAAGCTGAACGTGAGGAGACCACCAGCCCCGAGTGTGAGGAACACAATAACCTGAGGCCCGT GGTGCTCATGGAGGGGCTGTGCTCTGAGCAGGGGTACCTGGACCCTGTCTTGGTCAGCCCACCTGCGAAG AAGCCCTTGGAGAACAAGCCGGGAAAGCAAGAGGAGTTCCGTGCACATGGAACTCCGGACGACAGCTCTC TCCTGAGGAAGGACATCCGAGACCTGAAAGCCCAGCTACAGAATGCCAACAAGGTCATTCAGAACCTGAG GAGCCGGGTCCGGTCCCTGTCTGCCACAAGCGACTACTCATCAAGTCTGGAGAGACCCCGCAAGCTGAGA GCCGTGGCGACCCTTGAGGGGGCTTCACCCCACAGCGTGACTGATGAAGATGAGGGGTGGCTGTCAGATG GCACTGGGGCTTTTTACCCTCCAGGACTCCAGGCCAAAAAAGATCTAGAGAGTCTCATCCAGCGAGTATC CCAACTGGAGGCCCAGCTCCCCAAAACTGGACTAGAAGGGAAGCTGGCCGAGGAGCTGAGGTGTGCCTCG TGGCCTGGAAAATATGATTCCTTGATTCAGGATCAGGCCCGAGAACTATCATATCTGCGTCAAAAGATAC GAGAAGGGAGGGGGATATGTTATCTTCTCACCCAACATGCAAAAGATACTGTAAAATCTTTTGAGGACCT CCTTAGGAGCAACGACATTGACTACTACCTGGGCCAGAGCTTCCGGGAGCAACTAGCCCAGGGAGGTCAG CTGACGGAGAGGCTCACCAGCAAACTCAGCACAAAGGATCATAAGAGTGAAAAGGAAGAAGCTGGGCTTG AGCCACTGGCCCTCAGGCTCAGCAGGGAACTACAGGAGAAAGAGAAAGTAATTGAGGTCCTGCAGGCCAA GCTGGATACCCGGTCTCTCTCACCCCCAAGCAGCCACGCCGTGTCTGACTCCCACCGCTCCGCCAGCACC ACATCCTTCCTGTCGGATGACATAGAAGCCTGCTCTGACATGGACGTAGCCAGCGAGTACACACACTACG ACGAGAAGAAACCCTCACCCAGTCACTCAGCAGCCAGTGCATCTCAGGGGCTTAAGGGCGAGTCCAGCAG CAGCCCCATCAGCTTGCCAACTCCCCAGAATCCCCCTAAGGAGGCCAGCCAGGCCCATCCAGGCTTTCAC TTTCACTCCATACCCAAGCCGGCTAGCCTTTCCCAGACACCAATGCACTCCGCTCTGCCCAGCTTTGTGC CTTTCAGCCCCTCCGGGCCTCCCCTTCTGGGTTGCTGTGAGACACCGATGGTGTCCTTGGCTGAGGCTCA ACAAGAGCTGCAGATGCTGCAGAAGCAGCTGGGAGAAAGTGTTAGCATTGCCCCTCCCGCCTCCACATCC ACGCTGCTCAGCAACCAGACTGAAGCTAGCTCTCCCCACTACATCAACCCTGCCCAGCCCCACACTCCGA CAAGGAGCACCATAGAGCTGGGACGAATCCTGGAGCCTGGATACCTGGGAAGCAGCGGCCAGTGGGACAT GATGAGGCCCCAGAAAGGGAGCGTCTCTGGGGAGCTGTCCTCAGGCTCCTCGATGTACCAGCTTAACTCC AAGCCCACTGGCGCTGACCTGTTGGAAGAGCATTTAGGTGAGATCCGGAACCTGCGCCAGCGCCTGGAGG AGTCCATATGTGTCAATGACCGGCTGCGGGAGCAGCTGCAGCACAGGCTCAGCTCCACTGCCCGAGAAAA CGGTTCCACCTCTCACTTCTACAGTCAGGGCCTGGAGTCCATGCCTCAGCTCTACAATGAGAACAGAGCC CTAAGGGAAGAAAACCAAAGCCTGCAGACACGGCTCAGCCATGCTTCCAGAGGACACTCCCAGGAAGTGG ATCACCTGCGGGAGGCTCTGCTCTCCTCCAGATCCCAGCTTCAGGAGCTGGAGAAGGAGCTGGAGCAGCA GAAGGCTGAGCGGCAGCAGCTCCTGGAAGACTTGCAGGAGAAGCAGGATGAGATCGTGCAGTTCCGAGAG GAGAGGCTGTCGCTCCAGGAAAACAACTCCAGGCTGCAGCACAAGCTGGCCCTCCTGCAACAACAGTGCG AAGAGAAACAGCAGCTCTCCCTGTCCCTGCAGTCAGAGCTCCAGATCTACGAGTCCCTCTGTGAAAATCC CAAGAAGGCCTTGAAAGCTTTTAGCCTAGATTCCTGTCACCAAGTCCCGGGTGAGTTAAGCTGCCTGGTG GCAGAGATTCGAGCTCTGAGAGGACAGTTGGAGCAGAGCATTGAAGTGAACAACCGTCTTCGGCTACAGC TGGAACAGCAGATGGATCGTGGTGCTGGCAAAGCCAGTCTCGGCCCCATCGCTGTTGGCCAGAGCTTCCC AGACAAGGCAGAGCCAGCAAACCTGCACCAAGGTTCCGCTGCTTCCCCTCCAGTGCGGGACGTTGGCTTG AATTCCCCAGCCATGGTCCTCCCCAACTCTTCTTGCTCCGCTCCTGGCTCAGACCATGCCATTGTCACCA GGACAAACAACGAGCTAAGTTCAGATGATTCTGCAGCAATGAAGAACCCTCCCAAGCTGGAGGTCGATGC TACCGATGGCCCATTTGCCAACAAACACGGAAGACACGTCATCGGCCATGTTGATGACTACGATGCTCTG CAACAGCAGATTGGGGAAGGCAAGCTGCTGATTCAAAAGATACTGTCTCTCATGAGGTCAGCACGCAGCA TCCCTGGGCAAGAAGCTCAGGACACAGAGGCACCAGGTAACATAAGTGCCCATGAGCTTCGGAGCAGCGC CAAGGCTCTGAACCACGCCCTAGAAGAGTCAACATCCCTACTCAACATGTTCTGGAGAGCAGCCTTGCCA AACACTCATGGTCCTGTACTGGTAGGCAAAGAGGGACAACTGATGGAGAAAGAACTCTTGGACCTGCGAG CCCAAGTATCCCAACAGGAACAGATCCTTCAGAACACTGCTGCACGTCTGAAGAGGGCCAACCAGAGGAA GAAAAGCATGGAACAATTCATCGTGAGCCATTTGACTAGGACCCATGATGTCTTGAAGAAAGCGCGGACT AATTTAGAGATGAAATCCTTCAGGGCTCTGACGTGCACTCCGGCCTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR218661 representing NM_001110163 Red=Cloning site Green=Tags(s) MSNGYRTLSQHLNDLKKENFSLKLRIYFLEERMQQKYEVSREDVYKRNIELKVEVESLKRELQDRKQHLD KTWADAEDLNSQNEAELRRQVEERQQETEHVYELLGNKIQLLQEEPRLAKNEATEMETLVEAEKRCNLEL SERWTNAAKNREDAAGDQEKPDQYSEALAQRDRRIEELRQSLAAQEGLVEQLSQEKRQLLHLLEEPASME VQPVPKGLPTQQKPDLHETPTTQPPVSESHLAELQDKIQQTEATNKILQEKLNDLSCELKSAQESSQKQD TTIQSLKEMLKSRESETEELYQVIEGQNDTMAKLREMLHQSQLGQLHSSEGIAPAQQQVALLDLQSALFC SQLEIQRLQRLVRQKERQLADGKRCVQLVEAAAQEREHQKEAAWKHNQELRKALQHLQGELHSKSQQLHV LEAEKYNEIRTQGQNIQHLSHSLSHKEQLIQELQELLQYRDNADKTLDTNEVFLEKLRQRIQDRAVALER VIDEKFSALEEKDKELRQLRLAVRDRDHDLERLRCVLSANEATMQSMESLLRARGLEVEQLTATCQNLQW LKEELETKFGHWQKEQESIIQQLQTSLHDRNKEVEDLSATLLCKLGPGQSEVAEELCQRLQRKERMLQDL LSDRNKQAVEHEMEIQGLLQSMGTREQERQAAAEKMVQAFMERNSELQALRQYLGGKELMTSSQTFISNQ PAGVTSIGPHHGEQTDQGSMQMPSRDDSTSLTAREEASIPRSTLGDSDTVAGLEKELSNAKEELELMAKK ERESQMELSALQSMMAMQEEELQVQAADLESLTRNVQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLR EKVASVEPQGQEVSGNKRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAQPENSERDGTLQVELE GAQVLRTRLEEVLGRSLERLSRLESLAAIGGATAGNETEDTSTEFTDSIEEEAAHTSHQQLIKVALEKSL ATMETQNICLQPPSPVGEDSNRCLQEEMLHLRAEIHQHLEEKRKAEVELKELKAQIEEAGFSSVSHIRNT MLSLCLENAELKEQMGEAMSDGWEVEEDKEKGEVMLETVVAKGCLNENSLQAEFRKVQGKLKSAYNIINL LKEQLLLRSSEGNSKEMPELLVRLAREVDRMNTGLPSLGKHQHQEQENTTTARPGSRPQSLPLGAALSVD GYQLENKSQAQDSGHQPEFSLPGSTKHLRSQLAQCRQRYQDLQEKLLISEATVFAQANQLEKYRAVFSES LVKQDSKQIQVDLQDLGYETCGRSENEAEREETTSPECEEHNNLRPVVLMEGLCSEQGYLDPVLVSPPAK KPLENKPGKQEEFRAHGTPDDSSLLRKDIRDLKAQLQNANKVIQNLRSRVRSLSATSDYSSSLERPRKLR AVATLEGASPHSVTDEDEGWLSDGTGAFYPPGLQAKKDLESLIQRVSQLEAQLPKTGLEGKLAEELRCAS WPGKYDSLIQDQARELSYLRQKIREGRGICYLLTQHAKDTVKSFEDLLRSNDIDYYLGQSFREQLAQGGQ LTERLTSKLSTKDHKSEKEEAGLEPLALRLSRELQEKEKVIEVLQAKLDTRSLSPPSSHAVSDSHRSAST TSFLSDDIEACSDMDVASEYTHYDEKKPSPSHSAASASQGLKGESSSSPISLPTPQNPPKEASQAHPGFH FHSIPKPASLSQTPMHSALPSFVPFSPSGPPLLGCCETPMVSLAEAQQELQMLQKQLGESVSIAPPASTS TLLSNQTEASSPHYINPAQPHTPTRSTIELGRILEPGYLGSSGQWDMMRPQKGSVSGELSSGSSMYQLNS KPTGADLLEEHLGEIRNLRQRLEESICVNDRLREQLQHRLSSTARENGSTSHFYSQGLESMPQLYNENRA LREENQSLQTRLSHASRGHSQEVDHLREALLSSRSQLQELEKELEQQKAERQQLLEDLQEKQDEIVQFRE ERLSLQENNSRLQHKLALLQQQCEEKQQLSLSLQSELQIYESLCENPKKALKAFSLDSCHQVPGELSCLV AEIRALRGQLEQSIEVNNRLRLQLEQQMDRGAGKASLGPIAVGQSFPDKAEPANLHQGSAASPPVRDVGL NSPAMVLPNSSCSAPGSDHAIVTRTNNELSSDDSAAMKNPPKLEVDATDGPFANKHGRHVIGHVDDYDAL QQQIGEGKLLIQKILSLMRSARSIPGQEAQDTEAPGNISAHELRSSAKALNHALEESTSLLNMFWRAALP NTHGPVLVGKEGQLMEKELLDLRAQVSQQEQILQNTAARLKRANQRKKSMEQFIVSHLTRTHDVLKKART NLEMKSFRALTCTPAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001110163
ORF Size	6978 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001110163.1, NP_001103633.1
RefSeq Size	8289 bp
RefSeq ORF	6981 bp
Locus ID	83679
MW	262.8 kDa
Gene Summary	Functions as an anchor sequestering components of the cAMP-dependent pathway to Golgi and/or centrosomes (By similarity).[UniProtKB/Swiss-Prot Function]

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