Msx2 (NM_013601) Mouse Tagged ORF Clone
CAT#: MR223217
- TrueORF®
Msx2 (Myc-DDK-tagged) - Mouse homeobox, msh-like 2 (Msx2)
ORF Plasmid: tGFP
"NM_013601" in other vectors (4)
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CNY 3,600.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | BB122635; Hox-8; Hox8; Hox8.1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR223217 representing NM_013601
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCTCCGACTAAAGGCGGTGACTTGTTTTCGTCGGATGAGGAGGGCCCCGCGGTACTGGCCGGCC CGGGTCCCGGGCCTGGAGGAGCCGAGGGCAGCGCAGAGGAACGCAGGGTCAAGGTCTCCAGCCTGCCCTT CAGCGTGGAGGCGCTCATGTCCGACAAGAAGCCGCCCAAGGAATCGCCCGCGGTGCCACCCGACTGCGCC TCGGCTGGCGCTGTCCTGCGGCCGCTGCTGCTGCCGGGACACGGCGTCCGGGACGCTCACAGTCCCGGGC CTCTCGTCAAGCCCTTCGAGACCGCCTCGGTCAAGTCGGAAAATTCCGAAGACGGAGCACCGTGGATACA GGAGCCCGGCAGATACTCCCCGCCGCCCAGACATATGAGCCCCACCACCTGCACCCTGAGGAAACACAAG ACCAACCGGAAGCCACGCACACCCTTCACCACATCCCAGCTTCTAGCCTTGGAGCGCAAGTTCCGCCAGA AACAGTACCTGTCCATAGCAGAGCGGGCCGAGTTCTCCAGCTCTCTGAACCTTACAGAGACCCAGGTCAA AATCTGGTTCCAGAACCGAAGGGCTAAGGCGAAAAGACTGCAAGAGGCGGAACTGGAAAAGCTGAAAATG GCTGCCAAGCCTATGCTGCCCTCAGGCTTCAGTCTGCCCTTCCCTATCAACTCACCCCTGCAAGCAGCAT CCATATACGGCGCATCCTACCCCTTCCATAGACCTGTGCTCCCCATCCCGCCTGTTGGACTCTATGCCAC GCCGGTTGGATATGGCATGTACCATCTATCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR223217 representing NM_013601
Red=Cloning site Green=Tags(s) MASPTKGGDLFSSDEEGPAVLAGPGPGPGGAEGSAEERRVKVSSLPFSVEALMSDKKPPKESPAVPPDCA SAGAVLRPLLLPGHGVRDAHSPGPLVKPFETASVKSENSEDGAPWIQEPGRYSPPPRHMSPTTCTLRKHK TNRKPRTPFTTSQLLALERKFRQKQYLSIAERAEFSSSLNLTETQVKIWFQNRRAKAKRLQEAELEKLKM AAKPMLPSGFSLPFPINSPLQAASIYGASYPFHRPVLPIPPVGLYATPVGYGMYHLS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_013601 |
ORF Size | 801 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_013601.2, NP_038629.2 |
RefSeq Size | 2162 bp |
RefSeq ORF | 804 bp |
Locus ID | 17702 |
UniProt ID | Q03358 |
MW | 29.4 kDa |
Gene Summary | Acts as a transcriptional regulator in bone development. Represses the ALPL promoter activity and antagonizes the stimulatory effect of DLX5 on ALPL expression during osteoblast differentiation. Probable morphogenetic role. May play a role in limb-pattern formation. In osteoblasts, suppresses transcription driven by the osteocalcin FGF response element (OCFRE). Binds to the homeodomain-response element of the ALPL promoter.[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Direct Reprogramming of Mouse Fibroblasts into Functional Osteoblasts
,Zhu, H;Swami, S;Yang, P;Shapiro, F;Wu, JY;,
J. Bone Miner. Res.
,PubMed ID 31793059
[MSX2]
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Documents
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC208983 | Msx2 (untagged) - Mouse homeobox, msh-like 2 (Msx2), (10ug) |
CNY 3,600.00 |
|
MG223217 | Msx2 (tGFP-tagged) - Mouse homeobox msh-like 2 (Msx2), (10ug) |
CNY 4,370.00 |
|
MR223217L3 | Lenti ORF clone of Msx2 (Myc-DDK-tagged) - Mouse homeobox, msh-like 2 (Msx2) |
CNY 5,890.00 |
|
MR223217L4 | Lenti ORF clone of Msx2 (mGFP-tagged) - Mouse homeobox, msh-like 2 (Msx2) |
CNY 5,890.00 |