United States
Germany
Japan
United Kingdom
China
Phf8 (NM_177201) Mouse Tagged ORF Clone
CAT＃: MR223276
- TrueORF®
Phf8 (Myc-DDK-tagged) - Mouse PHD finger protein 8 (Phf8), transcript variant 1
ORF Plasmid: tGFP
"NM_177201" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 9830141C09Rik; mKIAA1111 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR223276 representing NM_177201
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCGGTGCCTGTGTATTGCCTCTGTCGACTGCCTTATGATGTGACCCGCTTCATGATCGAGTGTG ACATGTGCCAGGACTGGTTTCACGGCAGTTGTGTTGGTGTTGAGGAGGAGAAGGCTGCTGATATTGATCT TTACCACTGCCCTAACTGTGAGGTCTTACATGGACCTTCCATTATGAAAAAACGTCGTGGATCTTCCAAA GGACATGATAATCACAAGGGGAAGCCACTGAAGACTGGAAGCTCTATGTTTATCCGAGAACTTCGGGGCA GAACTTTTGACAGCTCAGATGAAGTGATTCTGAAGCCCACTGGAAGTCAGCTGACTGTGGAATTCTTGGA AGAGAATAGCTTCAGCGTGCCTATCCTTGTCTTGAAGAAGGATGGGTTGGGGATGACATTACCCTCTCCA TCATTCACTGTGAGGGATGTGGAACACTATGTTGGTTCTGACAAAGAGATTGATGTGATTGATGTGGCCC GCCAGGCTGACTGCAAGATGAAACTCGGTGATTTTGTCAAATACTATTACAGTGGGAAGAGGGAAAAAGT CCTCAATGTCATTAGTTTGGAATTCTCCGATACCAGGCTTTCAAACCTCGTGGAAACACCCAGGATTGTT CGCAAGCTGTCATGGGTGGAGAACTTGTGGCCAGAGGAATGTGTCTTTGAGAGACCCAATGTGCAGAAGT ACTGCCTCATGAGTGTGCGGGATAGCTATACAGATTTTCACATTGACTTTGGTGGGACCTCAGTTTGGTA CCATGTGCTTAAGGGTGAGAAGATCTTCTACCTGATCCGCCCAACAAATGCTAATCTGACTCTCTTTGAG TGCTGGAGTAGCTCCTCCAATCAGAACGAGATGTTCTTTGGTGACCAAGTGGAAAAGTGCTACAAGTGTT CTGTGAAGCAAGGACAAACACTGTTTATTCCTACAGGATGGATACATGCTGTGTTAACACCCGTGGACTG CTTAGCATTCGGAGGGAACTTCTTACACAGTCTTAACATTGAAATGCAACTCAAGGCTTATGAAATTGAG AAGAGGCTGAGCACAGCAGACCTTTTCAAGTTTCCCAACTTCGAGACCATCTGTTGGTATGTGGGAAAAC ATATTCTGGACATCTTTCGAGGCTTACGAGAAAATAGAAGACACCCTGCCTCCTACCTGGTCCATGGTGG TAAAGCTCTGAACTTGGCATTTAGAGCTTGGACAAAGAAAGAAGCTTTGCCAGACCACGAGGATGAGATC CCAGAGACAGTGCGGACTGTACAGCTCATTAAAGATCTGGCTAGGGAGATCCGTCTGGTTGAATTCAACA TGAGTGGTACCAGCTTGAATGATTCAGATGATGACTCAGCAGACATGGACCTTGATGGCAGTGAGAACCC TCTGGCCCTGTTGATGGCTAATGGCAGTACGAAGAGGATGAAGAGTGTATCCAAATCTCGGAGAGCCAAA ATTGCAAAGAAGGTAGACAGTGCAAGACTGGTAGCAGAACAGGTCATGGGAGATGAATTTGACTTGGATT CAGATGATGAGCTGCAGATTGACGAGAGATTGGGAAAGGAAAAGGCGAACCTGTTAATAAGATCAAAATT TCCCCGGAAGTTGCCCCGTGCAAAACCTTGCTCTGACCCCAACCGAATTCGTGAACCTGGAGAAGTTGAG TTTGACATTGAGGAGGACTATACCACAGATGAGGACATGGTGGAAGGGGTTGAAAGCAAGCTTGGGAATG GGAGTGGAGCCGGTGGAATTCTTGATCTACTTAAGGCCAGCAGGCAGGTGGGGGGACCTGACTATGCTGC CCTCACTGAGGCCCCAGCCTCCCCCAGCACTCAAGAGGCCATCCAGGGCATGTTGTGTATGGCCAACCTG CAGTCCTCATCATCCTCACCAGCTACCTCCAGTCTGCAGGCTTGGTGGACTGGAGGGCAAGAAAGAAGCA GCGGGAGCTCCAGCAGTGGCCTGGGCACTGTGTCTAGTAGTCCTGCTTCCCAGCGCACCCCAGGGAAGCG GCCCATCAAGAGGCCAGCATACTGGAAAAACGAGAGTGAAGAGGAGGAGAATGCCAGTCTTGATGAGCAA GACAGCTTGGGAGCATGCTTCAAGGATGCTGAGTATATCTATCCATCTCTGGAGTCTGATGATGATGACC CTGCTTTGAAATCTCGACCCAAGAAAAAGAAGAATTCAGATGATGCTCCATGGAGTCCTAAAGCCCGTGT GACTCCAACCCTACCCAAGCAGGACCGTCCTGTTCGTGAGGGGACCAGAGTTGCCTCCATTGAGACAGGC TTGGCTGCAGCAGCTGCAAAGCTTGCCCAGCAGGTAAGAGTTGACACCCAGCTGGTGGCCCTGCTACTGA TGACT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR223276 representing NM_177201
Red=Cloning site Green=Tags(s) MASVPVYCLCRLPYDVTRFMIECDMCQDWFHGSCVGVEEEKAADIDLYHCPNCEVLHGPSIMKKRRGSSK GHDNHKGKPLKTGSSMFIRELRGRTFDSSDEVILKPTGSQLTVEFLEENSFSVPILVLKKDGLGMTLPSP SFTVRDVEHYVGSDKEIDVIDVARQADCKMKLGDFVKYYYSGKREKVLNVISLEFSDTRLSNLVETPRIV RKLSWVENLWPEECVFERPNVQKYCLMSVRDSYTDFHIDFGGTSVWYHVLKGEKIFYLIRPTNANLTLFE CWSSSSNQNEMFFGDQVEKCYKCSVKQGQTLFIPTGWIHAVLTPVDCLAFGGNFLHSLNIEMQLKAYEIE KRLSTADLFKFPNFETICWYVGKHILDIFRGLRENRRHPASYLVHGGKALNLAFRAWTKKEALPDHEDEI PETVRTVQLIKDLAREIRLVEFNMSGTSLNDSDDDSADMDLDGSENPLALLMANGSTKRMKSVSKSRRAK IAKKVDSARLVAEQVMGDEFDLDSDDELQIDERLGKEKANLLIRSKFPRKLPRAKPCSDPNRIREPGEVE FDIEEDYTTDEDMVEGVESKLGNGSGAGGILDLLKASRQVGGPDYAALTEAPASPSTQEAIQGMLCMANL QSSSSSPATSSLQAWWTGGQERSSGSSSSGLGTVSSSPASQRTPGKRPIKRPAYWKNESEEEENASLDEQ DSLGACFKDAEYIYPSLESDDDDPALKSRPKKKKNSDDAPWSPKARVTPTLPKQDRPVREGTRVASIETG LAAAAAKLAQQVRVDTQLVALLLMT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_177201 |
| ORF Size | 2385 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_177201.5, NP_796175.1 |
| RefSeq Size | 3732 bp |
| RefSeq ORF | 2388 bp |
| Locus ID | 320595 |
| UniProt ID | Q80TJ7 |
| MW | 89.4 kDa |
| Gene Summary | Histone lysine demethylase with selectivity for the di- and monomethyl states that plays a key role cell cycle progression, rDNA transcription and brain development. Demethylates mono- and dimethylated histone H3 'Lys-9' residue (H3K9Me1 and H3K9Me2), dimethylated H3 'Lys-27' (H3K27Me2) and monomethylated histone H4 'Lys-20' residue (H4K20Me1). Acts as a transcription activator as H3K9Me1, H3K9Me2, H3K27Me2 and H4K20Me1 are epigenetic repressive marks. Involved in cell cycle progression by being required to control G1-S transition. Acts as a coactivator of rDNA transcription, by activating polymerase I (pol I) mediated transcription of rRNA genes. Required for brain development, probably by regulating expression of neuron-specific genes. Has activity toward H4K20Me1 only when nucleosome is used as a substrate and when not histone octamer is used as substrate. May also have weak activity toward dimethylated H3 'Lys-36' (H3K36Me2), however, the relevance of this result remains unsure in vivo. Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: has weak activity toward H3K9Me2 in absence of H3K4me3, while it has high activity toward H3K9me2 when binding H3K4me3.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221742 | Phf8 (untagged) - Mouse PHD finger protein 8 (Phf8), transcript variant 1, (10ug) |
CNY 10,260.00 |
|
| MG223276 | Phf8 (tGFP-tagged) - Mouse PHD finger protein 8 (Phf8) transcript variant 1, (10ug) |
CNY 6,370.00 |
|
| MR223276L3 | Lenti ORF clone of Phf8 (Myc-DDK-tagged) - Mouse PHD finger protein 8 (Phf8), transcript variant 1 |
CNY 7,700.00 |
|
| MR223276L4 | Lenti ORF clone of Phf8 (mGFP-tagged) - Mouse PHD finger protein 8 (Phf8), transcript variant 1 |
CNY 7,700.00 |
