United States
Germany
Japan
United Kingdom
China
Trim33 (NM_053170) Mouse Tagged ORF Clone
CAT＃: MR227454
- TrueORF®
Trim33 (Myc-DDK-tagged) - Mouse tripartite motif-containing 33 (Trim33), transcript variant 1
ORF Plasmid: tGFP
"NM_053170" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 11,904.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 8030451N04Rik; AI413936; Ecto; Tif1g |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR227454 representing NM_053170
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAAAACAAAGGCGGCGGTGAGGCAGAGAGTGGCGGCGGGGGCAGCGGTAGCGCGCCGGTAACCG CCGGGGCCGCCGGGCCCACGGCGCAGGAGGCGGAGCCGCCTCTCGCCGCGGTGCTGGTGGAGGAGGAGGA GGAGGAAGGCGGCAGGGCTGGCGCGGAGGGCGGTGCGGCTGGGCCCGACGACGGGGGGGTGGCCGCGGCC TCCTCAAGCTCGGCCCCGGCCGCCTCGGTCCCCGCGGCCTCAGTGGGCTCCGCGGTCCCCGGAGGCGCGG CATCGACGCCGGCTCCAGCTGCTGCCCCAGCCCCGGCCCCGGCCCCGGCCCCGGCCCCGGCCCCCGCTCC CGCCCCGGCTCCTGGTTCCTCGTCGGGGCCACCTCTCGGACCACCAGCCTCGCTTCTGGACACCTGCGCG GTGTGTCAGCAGAGCTTGCAGAGCCGGCGCGAGGCGGAGCCCAAGCTGCTCCCCTGCCTGCACTCCTTCT GCCTGCGCTGTCTGCCGGAGCCGGAGCGCCAGCTCAGCGTGCCCATCCCTGGGGGCAGCAACGGTGACGT CCAGCAAGTTGGTGTAATACGGTGTCCAGTATGTCGTCAAGAATGTAGACAGATAGATCTTGTGGATAAT TATTTTGTGAAAGACACATCTGAAGCTCCTAGCAGCTCTGATGAGAAATCAGAACAGGTATGTACTAGTT GTGAAGATAATGCAAGTGCAGTTGGTTTTTGTGTAGAATGTGGAGAGTGGCTGTGTAAGACATGCATTGA AGCACATCAAAGAGTAAAATTCACTAAAGACCACCTGATCAGGAAAAAAGAAGATGTCTCAGAGTCTGTT GGAACATCTGGTCAGCGCCCAGTGTTTTGTCCTGTACATAAACAAGAGCAGTTGAAACTATTCTGTGAAA CGTGTGATAGATTGACGTGTAGAGACTGTCAACTTTTGGAGCACAAAGAACATAGGTATCAGTTTTTAGA AGAGGCTTTTCAAAATCAGAAAGGTGCAATTGAAAATCTGTTGGCTAAGCTTCTTGAAAAGAAGAATTAT GTTCATTTTGCAGCTACACAAGTGCAGAATAGGATTAAAGAAGTAAATGAGACTAACAAACGTGTAGAAC AGGAAATTAAAGTGGCCATCTTCACCCTTATCAATGAAATTAATAAGAAAGGAAAATCTCTCTTGCAACA GCTAGAGAATGTTACAAAAGAAAGACAGATGAAGTTACTACAGCAGCAGAATGATATCACAGGCCTCTCC CGGCAGGTGAAGCATGTTATGAACTTTACAAACTGGGCCATTGCAAGTGGCAGCAGCACGGCCCTACTTT ATAGCAAGCGTCTGATTACTTTTCAGTTACGTCATATTTTGAAAGCCCGATGTGATCCTGTTCCTGCTGC CAATGGAGCAATTCGTTTCCATTGTGATCCCACCTTCTGGGCCAAAAATGTAGTGAATTTAGGTAATCTA GTAATAGAGAGTAAGCCAGCTCCTGGTTATACTCCTAATGTTGTAGTTGGGCAAGTTCCTCCAGGGACAA ACCACATTAGTAAAACCCCTGGACAGATTAATTTAGCACAGCTTCGACTCCAGCACATGCAGCAACAAGT GTATGCACAGAAACATCAGCAGTTACAACAGATGAGACTGCAGCAACCACCAGCTCCTATACCAACAACA ACAGCAACAACCCAGCAGCATCCTAGGCAAGCAGCCCCACAGATGTTACAACAACAGCCTCCAAGATTGA TCAGTGTGCAAACAATGCAAAGAGGCAACATGAACTGTGGAGCATTTCAAGCCCATCAAATGAGACTGGC TCAGAATGCTGCCAGAATACCAGGGATCCCCAGGCACAGTGCCCCTCAGTATTCCATGATGCAGCCACAT CTCCAAAGACAACATTCAAATCCAGGACATGCTGGACCGTTTCCTGTTGTATCAGCACACAACCCAATCA ACCCAACAAGTCCCACCACAGCAACTATGGCAAATGCAAATCGAGGTCCCACCAGCCCGTCTGTTACAGC AATAGAATTAATACCCTCAGTCACCAATCCAGAAAACCTTCCATCGTTGCCAGATATCCCACCTATACAG TTGGAAGATGCTGGCTCAAGTAGTTTAGATAATCTACTGAGTAGATATATCTCAGGCAGTCATCTACCCC CTCAGCCTACAAGTACCATGAATCCTTCCCCAGGACCCTCTGCGTTATCACCTGGATCTTCAGGCTTATC CAATTCTCACACACCTGTGAGACCCCCTAGCACGTCTAGTACTGGCAGTCGAGGCAGCTGCGGATCATCA GGAAGAACTGCTGAGAAAAGTGCTCATAGTTTCAAGTCTGATCAGGTGAAGGTCAAGCAGGAACCTGGGA CTGAAGAAGAGATATGCAGCTTTTCAGGAGCTGTGAAACAAGAGAAGACAGAGGATGGCAGGAGGAGTGC CTGTATGTTGAGCAGTCCTGAGAGTAGTTTGACACCACCTCTTTCAACTAACCTTCACCTAGAGAGTGAG CTGGATACATTAACAGGCCTGGAGAACCACGTGAAGACTGAACCTACAGATATCAGTGAAAGCTGCAAAC AGTCAGGACTCAGTAACCTAGTCAATGGAAAGTCTCCAATTCGAAACCTCATGCACAGGTCGGCAAGGAT TGGAGGGGATGGCAATAGCAAAGATGATGACCCAAATGAAGACTGGTGTGCCGTCTGCCAGAACGGAGGG GATCTTTTGTGTTGTGAGAAATGTCCGAAGGTCTTTCATCTTACCTGTCATGTTCCAACACTTCTCAGCT TTCCAAGTGGAGACTGGATATGCACATTTTGCAGAGATATTGGGAAGCCAGAAGTTGAATATGATTGTGA TAATATGCAGCATAGTAAGAAGGGGAAAACTGCACAAGGCTTAAGCCCTGTGGACCAAAGGAAATGTGAA CGTCTTCTGCTTTACCTCTATTGTCACGAATTAAGTATTGAATTCCAGGAGCCTGTTCCTGTTTCGATAC CAAACTACTATAAAATTATAAAGAAACCGATGGATTTATCAACGGTGAAGAAGAAGCTTCAGAAAAAACA TTCCCAGCATTACCAAATCCCAGATGACTTTGTGGCTGATGTCCGTTTGATCTTCAAGAACTGTGAAAGG TTTAATGAAATGATGAAAGTTGTTCAAGTTTATGCAGACACACAAGAGATTAATTTGAAGGGTGATTCAG AAGTAGCTAAGGCAGGGAAAGCAGTTGCATTGTACTTTGAAGATAAACTCTCAGAGATCTACTCAGACAG GACCTTCACTCCTTTGCCAGAGTTTGAGCAGGACGAAGATGATGGTGAAGTAACAGAAGACTCTGACGAA GATTTTATACAGCCTCGTAGAAAACGCCTAAAGTCAGACGAGAGACCAGTACATATAAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227454 representing NM_053170
Red=Cloning site Green=Tags(s) MAENKGGGEAESGGGGSGSAPVTAGAAGPTAQEAEPPLAAVLVEEEEEEGGRAGAEGGAAGPDDGGVAAA SSSSAPAASVPAASVGSAVPGGAASTPAPAAAPAPAPAPAPAPAPAPAPAPGSSSGPPLGPPASLLDTCA VCQQSLQSRREAEPKLLPCLHSFCLRCLPEPERQLSVPIPGGSNGDVQQVGVIRCPVCRQECRQIDLVDN YFVKDTSEAPSSSDEKSEQVCTSCEDNASAVGFCVECGEWLCKTCIEAHQRVKFTKDHLIRKKEDVSESV GTSGQRPVFCPVHKQEQLKLFCETCDRLTCRDCQLLEHKEHRYQFLEEAFQNQKGAIENLLAKLLEKKNY VHFAATQVQNRIKEVNETNKRVEQEIKVAIFTLINEINKKGKSLLQQLENVTKERQMKLLQQQNDITGLS RQVKHVMNFTNWAIASGSSTALLYSKRLITFQLRHILKARCDPVPAANGAIRFHCDPTFWAKNVVNLGNL VIESKPAPGYTPNVVVGQVPPGTNHISKTPGQINLAQLRLQHMQQQVYAQKHQQLQQMRLQQPPAPIPTT TATTQQHPRQAAPQMLQQQPPRLISVQTMQRGNMNCGAFQAHQMRLAQNAARIPGIPRHSAPQYSMMQPH LQRQHSNPGHAGPFPVVSAHNPINPTSPTTATMANANRGPTSPSVTAIELIPSVTNPENLPSLPDIPPIQ LEDAGSSSLDNLLSRYISGSHLPPQPTSTMNPSPGPSALSPGSSGLSNSHTPVRPPSTSSTGSRGSCGSS GRTAEKSAHSFKSDQVKVKQEPGTEEEICSFSGAVKQEKTEDGRRSACMLSSPESSLTPPLSTNLHLESE LDTLTGLENHVKTEPTDISESCKQSGLSNLVNGKSPIRNLMHRSARIGGDGNSKDDDPNEDWCAVCQNGG DLLCCEKCPKVFHLTCHVPTLLSFPSGDWICTFCRDIGKPEVEYDCDNMQHSKKGKTAQGLSPVDQRKCE RLLLYLYCHELSIEFQEPVPVSIPNYYKIIKKPMDLSTVKKKLQKKHSQHYQIPDDFVADVRLIFKNCER FNEMMKVVQVYADTQEINLKGDSEVAKAGKAVALYFEDKLSEIYSDRTFTPLPEFEQDEDDGEVTEDSDE DFIQPRRKRLKSDERPVHIK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_053170 |
| ORF Size | 3420 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_053170.3, NP_444400.2 |
| RefSeq Size | 8868 bp |
| RefSeq ORF | 3423 bp |
| Locus ID | 94093 |
| UniProt ID | Q99PP7 |
| MW | 124.1 kDa |
| Gene Summary | Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway (By similarity). May act as a transcriptional repressor (By similarity). Inhibits the transcriptional response to TGF-beta/BMP signaling cascade (By similarity). Plays a role in the control of cell proliferation (By similarity). Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor. Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade) (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Trim33 is required for appropriate development of pre-cardiogenic mesoderm
,Rajderkar, S;Mann, JM;Panaretos, C;Yumoto, K;Li, HD;Mishina, Y;Ralston, B;Kaartinen, V;,
Dev. Biol.
,PubMed ID 30940539
[TRIM33]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223715 | Trim33 (untagged) - Mouse tripartite motif-containing 33 (Trim33), transcript variant 1, (10ug) |
CNY 14,730.00 |
|
| MG227454 | Trim33 (tGFP-tagged) - Mouse tripartite motif-containing 33 (Trim33) transcript variant 1, (10ug) |
CNY 13,504.00 |
|
| MR227454L3 | Lenti ORF clone of Trim33 (Myc-DDK-tagged) - Mouse tripartite motif-containing 33 (Trim33), transcript variant 1 |
CNY 13,300.00 |
|
| MR227454L4 | Lenti ORF clone of Trim33 (mGFP-tagged) - Mouse tripartite motif-containing 33 (Trim33), transcript variant 1 |
CNY 14,304.00 |
