Trim3 (NM_001285871) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	BERP1; HAC1; Rnf22
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231039 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAAGAGGGAGGACAGCCCTGGCCCGGAGGTGCAGCCAATGGACAAGCAGTTTCTGGTATGCAGTA TCTGTCTGGATCGGTACCGGTGCCCCAAAGTTCTGCCTTGTCTACATACCTTCTGTGAAAGATGCCTCCA GAACTACATCCCTCCTCAGAGCCTGACACTGTCCTGTCCAGTGTGCCGGCAGACATCCATCCTCCCAGAA CAGGGCGTCTCAGCCCTACAAAACAACTTCTTCATCAGCAGCCTCATGGAGGCCATGCAGCAGGCACCTG AGGGGGCTCATGACCCTGAAGACCCCCACCCCCTCAGCGCAGTGGCTGGCCGCCCTCTCTCCTGCCCCAA CCATGAAGGCAAGACAATGGAGTTTTACTGTGAGGCCTGTGAGACTGCCATGTGTGGTGAGTGCCGCGCA GGGGAGCACCGTGAACACGGCACAGTGCTGTTGCGGGATGTGGTGGAGCAGCACAAGGCAGCTCTGCAGC GCCAGCTTGAGGCTGTGCGTGGCCGATTGCCACAGCTGTCTGCAGCTATCGCCTTGGTTGGGGGTATCAG CCAGCAGCTGCAAGAGCGCAAGGCAGAGGCCCTGGCTCAGATAAGTGCTGCCTTTGAGGACCTGGAGCAA GCTCTGCAGCAGCGCAAGCAGGCTCTGGTCAGCGACTTGGAGAGCATTTGTGGGGCCAAACAGAAGGTGT TGCAGACACAGTTAGACACACTGCGCCAGGGCCAGGAGCACATTGGCAGTAGCTGCAGCTTTGCAGAGCA GGCACTGAGACTGGGCTCTGCCCCTGAGGTTTTGCTAGTAAGAAAGCATATGCGAGAGAGGCTGGCTGCT CTGGCAGCTCAGGCCTTCCCAGAAAGGCCACATGAGAATGCCCAGCTGGAACTGGTCCTTGAAGTGGACG GGTTGCGGAGATCGGTGCTCAACCTGGGGGCACTGCTCACCACCAGTGCTACAGCCCATGAGACAGTGGC CACGGGCGAGGGCCTGCGCCAGGCACTAGTTGGCCAGCCTGCTTCTCTCACTGTCACCACCAAAGACAAA GATGGGCGGCTGGTGCGCACAGGCAGCGCAGAGCTGTGTGCAGAGATCACTGGCCCCGATGGTGTGCGCC TTGCAGTACCGGTGGTGGACCACAAAAATGGCACATACGAGCTGGTGTACACTGCACGCACAGAAGGTGA CCTGCTCCTCTCAGTGCTGCTCTATGGACAGCCGGTGCGTGGCAGCCCTTTTCGTGTGCGTGCCCTTCGA CCTGGGGACCTGCCACCTTCCCCAGATGATGTGAAGCGCCGGGTCAAGTCTCCCGGTGGTCCTGGCAGTC ATGTGCGCCAGAAGGCAGTTCGTAGGCCGAGCTCCATGTACAGCACTGGTGGCAAACGGAAGGACAATCC AATTGAAGACGAACTCGTCTTTCGTGTTGGTAGTCGTGGAAGGGAGAAGGGTGAATTCACCAATTTACAA GGTGTGTCCGCTGCTAGCAGTGGCCGCATCGTGGTAGCAGACAGCAACAACCAATGTATTCAGGTTTTCT CCAATGAGGGCCAGTTCAAGTTCCGATTTGGGGTCCGTGGGCGCTCACCTGGGCAACTACAGCGTCCTAC AGGTGTGGCAGTGGACACCAATGGAGACATTATTGTGGCAGACTATGACAACCGTTGGGTCAGCATCTTC TCTCCTGAAGGCAAGTTCAAGACCAAGATTGGAGCTGGCCGCCTCATGGGACCCAAGGGAGTGGCTGTGG ACCGAAATGGACATATCATTGTGGTGGATAACAAATCTTGCTGTGTCTTCACCTTCCAGCCCAATGGCAA GCTTGTTGGGCGTTTTGGAGGCCGTGGGGCCACTGACCGCCACTTTGCAGGGCCCCACTTTGTGGCTGTG AACAACAAGAATGAGATTGTAGTAACGGATTTCCATAACCATTCAGTGAAGGTGTACAGTGCTGACGGAG AGTTCCTCTTCAAATTTGGCTCGCATGGCGAGGGCAACGGACAGTTCAATGCCCCCACGGGAGTAGCTGT GGATTCCAATGGGAACATCATCGTGGCCGACTGGGGCAACAGCCGCATACAGGTATTCGACAGCTCTGGT TCCTTCCTGTCCTATATCAACACGTCTGCAGAGCCACTATATGGCCCACAGGGCTTGGCATTGACCTCGG ATGGCCACGTGGTAGTGGCTGATGCTGGCAACCACTGCTTTAAGGCCTATCGATACCTCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231039 protein sequence Red=Cloning site Green=Tags(s) MAKREDSPGPEVQPMDKQFLVCSICLDRYRCPKVLPCLHTFCERCLQNYIPPQSLTLSCPVCRQTSILPE QGVSALQNNFFISSLMEAMQQAPEGAHDPEDPHPLSAVAGRPLSCPNHEGKTMEFYCEACETAMCGECRA GEHREHGTVLLRDVVEQHKAALQRQLEAVRGRLPQLSAAIALVGGISQQLQERKAEALAQISAAFEDLEQ ALQQRKQALVSDLESICGAKQKVLQTQLDTLRQGQEHIGSSCSFAEQALRLGSAPEVLLVRKHMRERLAA LAAQAFPERPHENAQLELVLEVDGLRRSVLNLGALLTTSATAHETVATGEGLRQALVGQPASLTVTTKDK DGRLVRTGSAELCAEITGPDGVRLAVPVVDHKNGTYELVYTARTEGDLLLSVLLYGQPVRGSPFRVRALR PGDLPPSPDDVKRRVKSPGGPGSHVRQKAVRRPSSMYSTGGKRKDNPIEDELVFRVGSRGREKGEFTNLQ GVSAASSGRIVVADSNNQCIQVFSNEGQFKFRFGVRGRSPGQLQRPTGVAVDTNGDIIVADYDNRWVSIF SPEGKFKTKIGAGRLMGPKGVAVDRNGHIIVVDNKSCCVFTFQPNGKLVGRFGGRGATDRHFAGPHFVAV NNKNEIVVTDFHNHSVKVYSADGEFLFKFGSHGEGNGQFNAPTGVAVDSNGNIIVADWGNSRIQVFDSSG SFLSYINTSAEPLYGPQGLALTSDGHVVVADAGNHCFKAYRYLQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001285871
ORF Size	2232 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001285871.1, NP_001272800.1
RefSeq Size	3077 bp
RefSeq ORF	2235 bp
Locus ID	55992
UniProt ID	Q9R1R2
MW	80.8 kDa
Gene Summary	Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (By similarity). Positively regulates motility of microtubule-dependent motor protein KIF21B (PubMed:24086586).[UniProtKB/Swiss-Prot Function]

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