Znrf3 (NM_001290501) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Gm1167
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231191 representing NM_001290501 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGCACCCACTAGGCCTATGTAATAACAATGACGAAGAGGACTTGTATGAATATGGCTGGGTAGGAG TGGTGAAGCTTGAACAGCCAGAATTGGACCCCAAACCATGTCTCACTGTCCTGGGCAAGGCCAAGAGAGC AGTTCAGCGGGGAGCTACTGCAGTTATCTTTGATGTATCTGAAAATCCAGAAGCCATCGACCAGCTAAAC CAAGGCTCAGAAGACCCTCTTAAGAGGCCAGTGGTGTATGTGAAGGGTGCAGATGCTATCAAGCTGATGA ACATTGTCAACAAGCAGAAAGTAGCTCGAGCAAGGATCCAGCACCTCCCTCCTCGACAACCCACCGAGTA CTTTGACATGGGAATTTTCCTGGCCTTCTTCGTCGTGGTCTCCTTGGTCTGCCTCATTCTCCTTGTCAAA ATCAAGCTGAAGCAACGTCGTAGTCAGAATTCCATGAACAGGCTGGCTGTGCAGGCTCTGGAGAAGATGG AAACCAGAAAGTTCAACTCCAAGAGCAAGGGGCGCCGGGAAGGAAGCTGTGGGGCCCTGGACACACTGAG CAGCGGCTCCACATCCGACTGTGCCATCTGTCTGGAGAAGTACATTGACGGAGAGGAGCTTCGGGTCATC CCTTGTACTCATCGGTTCCACAGGAAGTGTGTGGATCCATGGCTACTGCAGCACCACACCTGCCCCCACT GTCGGCACAACATCATAGAACAAAAGGGAAATCCAGGTGCTGTTTGTGTGGAGACAAGCAACCTTACACG TGGTCGGCAGCCAAGAGTGACCCTACCGGTACATTACCCAGGCCGTGTGCATAGGACCAATGCCATCCCA GCCTACCCTACTAGGACAAGTATGGACTCCCATGGCAACCCTGTCACACTGCTAACCATGGACCGGCATG GGGAGCAGAACCTCTATTCTCCACAAACACCCACCTATGTCCGTGGCTACCCACCCCTGCACCTGGACCA CACTCTGGCCCCTCATCGCTGCAGCCTGGAACACCGGGCCTACTCACCAGCCCATCCCTTCCGCAGGCCC AAGTTCAGCAGCCGCAGCTTCTCCAAGGCAGCTTGCTTCTCCCAGTATGAGACCATGTACCAACACTACT ACTTCCAGGGTCTCAGCTACCCAGAGCAGGAGGGCCAGACAATACCCAGCGTCACACCCAGGGGCCAATC CCGTGCCTTCCCACCAAGTGGTGCCAGCAGCCTGCTCTTCCCCACAATGGTACATGTGGCCCCTCCAACC CACGTGGAGAGTGGCAGCACATCTAGCTTCAGTTGCTATCATGGGCACCGTTCTGTGTGTAGCGGCTACC TGGCTGACTGTCCTGGCAGCGATAGCAGCAGCAACAGCTCTGGCCAGTGCCGCTGCTCTTCCAGTGACTC CGTGGTGGACTGCACAGAAGTCAGCAACCAAGGTGTGTATGGGAGCTGTTCCACCTTCCGAAGCTCCCTC AGCAGCGACTATGACCCCTTCATCTACCGCAGCCGGGGGCCTGCTGTGCACCTTGAGGGCTCCCCTCCAC CAGAGGAGCTCCCAGCAGGACACAGTCAAAGTGCTGGGAGGGGCGAGCCATGGCTAGGCCCTGCCTCTCC CTCAGGGGACCAGCTGTCCACCTGCAGCCTGGAGATGAACTACAGCAGCAACTCCTCGCTGGAACCCAGA GGGCCCAACAGCTCTACCTCAGAAGTGGGGCTCGAGGTTTCTCCTGGGGCTGCCCTGGACCTCAGGAGGA CCTGGAAAGGGGGTCCAGAGGGACCATCATGTGCTTGCTGCTTCGAGCCCCAGCCCTTCCCGCCGGGCTC TGGGATAGAAACAAGTGCAGGTGGCAGCAGTTTATTCCTGGGTCCTCGGCTCCTCGAGGACTGCAACCCT CCAAGTGGGGAGCCACAGCTGGGGAGCTCCCAGGGCCTGTATGGTCTTCACTCAGACCATTATCCTAGGA CAGATGGGGTGAAATATGAGGGCTTGCCCTGCTGCTTCTATGAAGAGAAGCAGGTGGCCCACAGTGCTGG CAGGGGCAATGGCTGCTACACTGAGGACTATTCAGTGAGTGTACAGTACACACTCACCGAGGAACCCCCA CCCAGCTGTTATGCAGGGCCCCGGGACCTGAGCCAGCGCATCCCCATTATTCCAGAGGATGTAGACTGTG ACTTGGGCTTGCCCCAAGACTGCCACGGGATGCACAACCACAGCCCCTGGGGTGGGGCACTGAGCTTGGA CGTTCCCCGACTCCACTGGAGTCTAGGCACAACCCGGGAAGAGGAACAGGCTCCATGTTACCAAGCTGAG GTGCAGCCTGGCTGCTCTCCAGAGGAGGCAGGTGCTTCCAGGGCTAGCCTCTCCAGTGCCCCCCAGGACA CTCAGGAGTCTCATGCCCTGGCTGCTGAGGCTTCAGGTGAGAACAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231191 representing NM_001290501 Red=Cloning site Green=Tags(s) MMHPLGLCNNNDEEDLYEYGWVGVVKLEQPELDPKPCLTVLGKAKRAVQRGATAVIFDVSENPEAIDQLN QGSEDPLKRPVVYVKGADAIKLMNIVNKQKVARARIQHLPPRQPTEYFDMGIFLAFFVVVSLVCLILLVK IKLKQRRSQNSMNRLAVQALEKMETRKFNSKSKGRREGSCGALDTLSSGSTSDCAICLEKYIDGEELRVI PCTHRFHRKCVDPWLLQHHTCPHCRHNIIEQKGNPGAVCVETSNLTRGRQPRVTLPVHYPGRVHRTNAIP AYPTRTSMDSHGNPVTLLTMDRHGEQNLYSPQTPTYVRGYPPLHLDHTLAPHRCSLEHRAYSPAHPFRRP KFSSRSFSKAACFSQYETMYQHYYFQGLSYPEQEGQTIPSVTPRGQSRAFPPSGASSLLFPTMVHVAPPT HVESGSTSSFSCYHGHRSVCSGYLADCPGSDSSSNSSGQCRCSSSDSVVDCTEVSNQGVYGSCSTFRSSL SSDYDPFIYRSRGPAVHLEGSPPPEELPAGHSQSAGRGEPWLGPASPSGDQLSTCSLEMNYSSNSSLEPR GPNSSTSEVGLEVSPGAALDLRRTWKGGPEGPSCACCFEPQPFPPGSGIETSAGGSSLFLGPRLLEDCNP PSGEPQLGSSQGLYGLHSDHYPRTDGVKYEGLPCCFYEEKQVAHSAGRGNGCYTEDYSVSVQYTLTEEPP PSCYAGPRDLSQRIPIIPEDVDCDLGLPQDCHGMHNHSPWGGALSLDVPRLHWSLGTTREEEQAPCYQAE VQPGCSPEEAGASRASLSSAPQDTQESHALAAEASGENR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290501
ORF Size	2427 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290501.1, NP_001277430.1
RefSeq Size	7199 bp
RefSeq ORF	2430 bp
Locus ID	407821
UniProt ID	Q5SSZ7
MW	88.8 kDa
Gene Summary	E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination and subsequent degradation of Wnt receptor complex components Frizzled and LRP6. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone (PubMed:22575959, PubMed:22895187). Along with RSPO2 and RNF43, constitutes a master switch that governs limb specification (By similarity).[UniProtKB/Swiss-Prot Function]

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