Inpp4b (NM_001297591) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	E130107I17Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231405 representing NM_001297591 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAATCAAAGAGGAAGGAACATCAGAAGAAGGCCAGCATTTTCTTCCTGCAGCTCAGGCAAATGACC CTGAGGACATTCAGTTTACCAGCATCCAGAAGATTCCGAACGAGCCACAGTTGGAATTCATTCTCGCCTG CAGGGATCTTGTGGCTCCTGTCAGTGATCGGAAACTGAATACAGTAGTCCAGATCTCAGTAATCCACCCG GTGGAGCAGACCCTGACGAGGTACTCCAGCACAGAAATTGTGGAGGGAACAAAAGACCCACTGTTCCTAA CGGGTGTCACATTCCCACCGGACTACCCCATCTATGAAGAGACAAGAATAAAACTGACAGTCTACGATGT CAAGGATAAGTCTCATGATACTATTCGAACCAGTGTCCTTCCGGAACACAAGGATCCCCTGCCAGAAGTT GCGAGAAGCTTCCTGGGCTGTGCCAGTTTTAAAGTTGGGGAGCTGCTGAAGTCCAAGGAGCAGCTGCTGT CCCTGAGCCTGAGAACCTCAGATGGTGGCAAAGTGGTCGGCACCATAGAAGTCAGCCTCGTGAAGATGGG AGAGATTGAGGATGGGGACACCGACCACATCACAACAGATGTGCAGGGACAAAAGTGTGCACTAATGTAT GAATCCACAGCACCAGAAAGTCTGAGCGGGAAGGAAAACTTACCTTTTATGAATGCAGTCTTAAGGAATC CTGTGTGTAAGCTGTATAGATTCCCCACATCTGACAATAAGTGGATGCGAATCCGTGAGCAGATGTCAGA AAGCATCCTTTCTTTTCATATTCCTAAGGAATTGATTTCCCTTCACATAAAAGAAGACTTATGTAGAAAC CAGGAGTTAAAAGAACTTGGTGACCTTTCCCCACACTGGGACAACCTACGGAAAAATGTCCTTTCTCACT GTGATCAAATGGTGACTATGTACCAAGACATTCTGACAGAACTTTCCAAAGAAACAGGGTCCTCTTTCAA ATCCAGCAGCAGCAAAGGAGAGAAAACATTAGAATTTGTTCCAGTAAATCTACACTTGCAACGGATGCAG GTGCACAGCCCTCACCTGAAAGATGCTCTCTATGATGTCATCACTGTGGGGGCCCCAGCTGCCCATTTTC AGGGATTCAAGAACGGTGGTCTTCGTAAACTCCTCCATAGGTTTGAGACAGAGAGAAGGAACACCGGCTA CCAGTTTATTTACTATTCACCTGAGAACACAGCGAAGGCAAAGGAAGTTCTCAGCAGCATCAATCAGCTA CAACCTCTCATAGCAACTCATGCAGACCTGCTGCTAACCTCTGCCAGCCAGCGTTCTCCAGACAGCCTGA AGAGCTCTTTAAAGCTGCTTTCAGAAAAAACTGAGCTTTTTGTCCATGCCTTCAAGGACCAGCTGGTCAG GAGTGCTCTTTTAGCTCTCTACACTGCAAGGCCAGGAGGCATCCTTAAGAAGCCACCCTCTCCTAATGTC AGCACAGAGGAGAAAAGTACTCAGCATGATACCCCACAGCTTAGAAGACAAGACTCCATACCACATCATT CTGACTATGATGAAGAAGAGTGGGATAGGGTGTGGGCCAACGTGGGGAAGAGCCTGAACTGCATTATCGC TAAAGTAGACAAACTGATCGAGAGAGACAGTCACAACGAAGAAGGTGCTGGAGGCAGCAGTAGCAAGGAT GGAGAGGCAGACCATACCCTGGAAGATTCCATCACATCTCACCCACGTGAGGACTGGTATGAACAGCTGC ACCCACTCATCCTTACCTTGAAGGAGTGCATGGGAGAAGTGGTGAACCGAGCAAAGCAATCCCTGACATT TGTACTGCTTCAGGAACTGGCCTACAGTCTGCCCCAGTGTTTGATGCTGACTCTGAGAAGAGACATTGTC TTCAGTCAGGCGCTTGCTGGATTGGTTTGTGGTTTTATCATCAAATTACATACAAGTTTGCATGACCCTG GCTTCCTACAGCAGCTTCATACAGTGGGCTTGATAGTACAATACGAAGGACTGCTAAGTACTTACAGTGA TGAAATTGGAATGCTGGAGGACATGGCAGTCGGCATTTCAGATTTGAGGAAAGTTGCATTTAAAATAACT GAAGCCACGTCCAATGATGTCCTTCCTGTTCTCACGGGGAGACGGGAACACTATGTGGTGGAGGTCAAGC TTCCAGCTACAGTATTTGAGTCGCTCCCACTGCAGATTAAAGAAGGCCAGTTGCTTCATGTGTATCCCGT TCTGTTTAATGTCGGAATCAATGAGCAGCAGACTCTGGCTGAGAGGTTTGGAGATGTTTCTTTGCAAGAA AGTATTAATCAAGAAAATTTTGAACTTGTACAAGAATATTATTCAATATTTATGGAAAAGATGCCTCCTG ATTATATTTCACATTTTCAAGAACAAACTGATTTAAAAGGACTGTTGGACAATCTCCACCAAAATATTCA AGCCAAAAAAAGAAAGAATGTAGAAATCATGTGGCTGGCAGCAACGATTTGTCGGAAACTCAATGGCATT CGTTTTACCTGCTGTAAAAGTGCCAAAGACAGGACATCGATGTCGGTGACACTGGAACAGTGCTCCATCC TGAGAGACGAGCATCAACTGCACAAAGACTTTTTCATCAGAGCTTTAGATTGCATGAGAAGCAGACAAAC CCAGGGAGCCTTGAATGAATCCGATGATCCTGAGACAGGCTGCCTAACTGATAACAAGCCAACTTCTCGA CACTTCTATCCTGTCGCCTTGCTTCTTGTCAGCTCACACCTGCTAGTTGTGTGGCTTATTTTAAGTCTTG CATTACTCTTAGCCAAATACCAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231405 representing NM_001297591 Red=Cloning site Green=Tags(s) MEIKEEGTSEEGQHFLPAAQANDPEDIQFTSIQKIPNEPQLEFILACRDLVAPVSDRKLNTVVQISVIHP VEQTLTRYSSTEIVEGTKDPLFLTGVTFPPDYPIYEETRIKLTVYDVKDKSHDTIRTSVLPEHKDPLPEV ARSFLGCASFKVGELLKSKEQLLSLSLRTSDGGKVVGTIEVSLVKMGEIEDGDTDHITTDVQGQKCALMY ESTAPESLSGKENLPFMNAVLRNPVCKLYRFPTSDNKWMRIREQMSESILSFHIPKELISLHIKEDLCRN QELKELGDLSPHWDNLRKNVLSHCDQMVTMYQDILTELSKETGSSFKSSSSKGEKTLEFVPVNLHLQRMQ VHSPHLKDALYDVITVGAPAAHFQGFKNGGLRKLLHRFETERRNTGYQFIYYSPENTAKAKEVLSSINQL QPLIATHADLLLTSASQRSPDSLKSSLKLLSEKTELFVHAFKDQLVRSALLALYTARPGGILKKPPSPNV STEEKSTQHDTPQLRRQDSIPHHSDYDEEEWDRVWANVGKSLNCIIAKVDKLIERDSHNEEGAGGSSSKD GEADHTLEDSITSHPREDWYEQLHPLILTLKECMGEVVNRAKQSLTFVLLQELAYSLPQCLMLTLRRDIV FSQALAGLVCGFIIKLHTSLHDPGFLQQLHTVGLIVQYEGLLSTYSDEIGMLEDMAVGISDLRKVAFKIT EATSNDVLPVLTGRREHYVVEVKLPATVFESLPLQIKEGQLLHVYPVLFNVGINEQQTLAERFGDVSLQE SINQENFELVQEYYSIFMEKMPPDYISHFQEQTDLKGLLDNLHQNIQAKKRKNVEIMWLAATICRKLNGI RFTCCKSAKDRTSMSVTLEQCSILRDEHQLHKDFFIRALDCMRSRQTQGALNESDDPETGCLTDNKPTSR HFYPVALLLVSSHLLVVWLILSLALLLAKYQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001297591
ORF Size	2823 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001297591.1, NP_001284520.1
RefSeq Size	4406 bp
RefSeq ORF	2826 bp
Locus ID	234515
MW	106.9 kDa
Gene Summary	Catalyzes the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate and inositol 3,4-bisphosphate (By similarity). Plays a role in the late stages of macropinocytosis by dephosphorylating phosphatidylinositol 3,4-bisphosphate in membrane ruffles (By similarity). Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival (By similarity).[UniProtKB/Swiss-Prot Function]

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