Pcsk6 (NM_001291184) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	b2b2830Clo; C86343; Pace4; SPC4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231414 representing NM_001291184 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCCGCGCGCGCCGCCAGCGCCCGGGCCCCGGCCGCCGCCCCGGGCCGCCGATGTCGCGCGGGGCG CAGGGGCCGCCGGCCGGCACGGACTCCCGCCGCTCGCGCTGCGCCCCTGGCGTTGGCTGCTTCTGCTCGC GCTGCCCGCCGCCTGCTCCGCGCTGCCGCCGCCGCGCCCCGTCTACACCAACCACTGGGCAGTGCAAGTG CTGGGCGGCCCCGGCGCGGCGGACCGCGTGGCTGCGGCGCACGGCTACCTCAACTTGGGCCAGATTGGAA ACCTGGACGATTACTATCATTTTTACCACAGCAAGACCTTCAAGAGATCGACCTTGAGCAGCAGGGGCCC CCACACCTTCCTCAGAATGGACCCACAGGTAAAATGGCTGCAACAACAGGAAGTGAAGCGCAGAGTCAAG AGACAGGCGCGAAGTGACTCTCTTTATTTCAATGATCCCATTTGGTCTAACATGTGGTACATGCATTGTA CTGATAAGAACAGTCGCTGTCGGTCAGAGATGAACGTCCAGGCGGCGTGGAAGCGTGGCTACACAGGAAA GAATGTGGTCGTCACCATCCTCGATGACGGCATAGAAAGGAATCACCCAGACCTGGCCCCCAACTACGAT TCCTATGCAAGCTACGATGTCAATGGAAATGATTATGACCCATCCCCGAGATATGACGCCAGCAACGAGA ACAAACATGGCACTCGCTGTGCGGGTGAAGTCGCTGCCTCGGCCAACAACTCCTACTGCATCGTGGGCAT CGCATATAATGCAAAGATAGGAGGCATCCGGATGCTGGACGGCGATGTGACCGATGTGGTGGAGGCCAAG TCTCTGGGCATCAGACCCAACTACATTGACATTTACAGCGCCAGTTGGGGACCCGATGATGACGGAAAGA CAGTAGATGGACCAGGCCGGCTGGCTAAACAAGCTTTCGAGTATGGCATTAAAAAGGGTCGCCAAGGTCT GGGCTCCATTTTTGTTTGGGCCTCTGGGAATGGTGGGAGAGAAGGGGACCACTGCTCCTGTGATGGCTAC ACCAACAGCATCTACACCATCTCCGTAAGCAGCACCACTGAGAACGGCCACAAACCCTGGTACCTGGAAG AATGCGCTTCCACGTTGGCTACCACCTACAGCAGCGGGGCCTTCTATGAACGGAAGATCGTCACCACAGA TCTGCGTCAGCGCTGCACCGACGGCCACACTGGGACATCAGTCTCAGCCCCCATGGTGGCTGGTATCATT GCCTTGGCTCTAGAAGCAAACAACCAGTTGACCTGGAGGGATGTGCAGCACCTGCTGGTGAAGACATCAC GGCCAGCTCATCTGAAGGCGAGCGACTGGAAAGTCAACGGAGCTGGGCATAAAGTTAGCCATCTCTATGG ATTTGGCTTGGTGGATGCAGAAGCTCTGGTCCTGGAGGCGAGGAAGTGGACGGCAGTGCCCTCCCAGCAC GTATGCGTGGCCACCGCAGACAAAAGGCCCAGGAGCATCCCCATAGTGCAGGTGCTACGGACCACAGCCC TGACCAACGCCTGTGCGGACCACTCAGACCAGCGTGTGGTGTACCTGGAGCATGTGGTTGTCCGAATCTC TATCTCACACCCACGCCGGGGCGATCTCCAGATCCACCTGATTTCTCCCTCTGGAACCAAGTCTCAACTT TTGGCAAAGAGATTGCTGGATTTTTCCAATGAGGGGTTCACAAACTGGGAATTCATGACCGTCCACTGCT GGGGAGAAAAGGCTGAAGGGGAGTGGACTCTGGAAGTGCAGGATATACCATCGCAGGTCCGAAACCCAGA GAAACAAGGAAAGCTGAAAGAATGGAGCCTCATTTTATATGGCACCGCAGAGCACCCATATCGCACCTTC AGCTCCCATCAGTCTCGCTCACGGATGCTGGAGCTCTCCGTCCCGGAGCAGGAGCCTCCCAAGGCTGCTG GACAACCACCCCAGGCAGAGACTCCAGAAGACGAGGAAGAGTACACAGGTGTGTGCCATCCCGAGTGTGG TGACAAAGGCTGTGATGGTCCCAATGCAGACCAGTGCTTGAACTGTGTCCACTTCAGCCTGGGGAACTCC AAGACAAACAGGAAGTGTGTGAGCGAGTGCCCCTTGGGCTACTTTGGGGATGCAGCTGCAAGACGCTGCC GTCGCTGCCATAAGGGATGTGAGACGTGCACGGGCAGGAGCCCAGCACAGTGCCTGTCTTGTCGCCGTGG GTTCTATCACCACCAGGAGACGAACACGTGTGTGACCCTCTGTCCTGCCGGACTTTATGCTGATGAAAGT CAGAGACTCTGCCTCAGGTGCCACCCGAGCTGTCAGAAGTGCGTGGATGAACCTGAGAAGTGCACTGTGT GTAAGGAGGGATTCAGCCTGGCACGGGGCAGCTGCATTCCGGACTGTGAACCGGGTACCTACTTCGATTC AGAGCTCGTCAAATGTGGGGAATGCCATCACACCTGCCGGACCTGTGTGGGGCCCAGCAGAGAAGAGTGT ATTCACTGTGCAAAAAGCTTCCACTTCCAAGACTGGAAATGTGTGCCGGCCTGCGGTGAGGGCTTCTACC CGGAGGAGATGCCTGGCTTACCCCACAAAGTGTGTCGAAGATGTGAGGAGAACTGCCTGAGCTGCGAGGG CTCCAGTAGGAACTGCAGCAGATGTAAAGCCGGCTTCACACAGCTGGGAACCTCCTGCATCACCAACCAC ACGTGCAGCAATGCCGATGAGACCTTCTGCGAGATGGTGAAGTCCAATCGGCTCTGCGAACGGAAGCTCT TCATCCAGTTTTGCTGCCGCACCTGCCTCCTGGCTGGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231414 representing NM_001291184 Red=Cloning site Green=Tags(s) MPPRAPPAPGPRPPPRAADVARGAGAAGRHGLPPLALRPWRWLLLLALPAACSALPPPRPVYTNHWAVQV LGGPGAADRVAAAHGYLNLGQIGNLDDYYHFYHSKTFKRSTLSSRGPHTFLRMDPQVKWLQQQEVKRRVK RQARSDSLYFNDPIWSNMWYMHCTDKNSRCRSEMNVQAAWKRGYTGKNVVVTILDDGIERNHPDLAPNYD SYASYDVNGNDYDPSPRYDASNENKHGTRCAGEVAASANNSYCIVGIAYNAKIGGIRMLDGDVTDVVEAK SLGIRPNYIDIYSASWGPDDDGKTVDGPGRLAKQAFEYGIKKGRQGLGSIFVWASGNGGREGDHCSCDGY TNSIYTISVSSTTENGHKPWYLEECASTLATTYSSGAFYERKIVTTDLRQRCTDGHTGTSVSAPMVAGII ALALEANNQLTWRDVQHLLVKTSRPAHLKASDWKVNGAGHKVSHLYGFGLVDAEALVLEARKWTAVPSQH VCVATADKRPRSIPIVQVLRTTALTNACADHSDQRVVYLEHVVVRISISHPRRGDLQIHLISPSGTKSQL LAKRLLDFSNEGFTNWEFMTVHCWGEKAEGEWTLEVQDIPSQVRNPEKQGKLKEWSLILYGTAEHPYRTF SSHQSRSRMLELSVPEQEPPKAAGQPPQAETPEDEEEYTGVCHPECGDKGCDGPNADQCLNCVHFSLGNS KTNRKCVSECPLGYFGDAAARRCRRCHKGCETCTGRSPAQCLSCRRGFYHHQETNTCVTLCPAGLYADES QRLCLRCHPSCQKCVDEPEKCTVCKEGFSLARGSCIPDCEPGTYFDSELVKCGECHHTCRTCVGPSREEC IHCAKSFHFQDWKCVPACGEGFYPEEMPGLPHKVCRRCEENCLSCEGSSRNCSRCKAGFTQLGTSCITNH TCSNADETFCEMVKSNRLCERKLFIQFCCRTCLLAG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001291184
ORF Size	2838 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291184.1, NP_001278113.1
RefSeq Size	4259 bp
RefSeq ORF	2841 bp
Locus ID	18553
MW	105.2 kDa

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