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TFIIF (GTF2F2) (NM_004128) Human Tagged ORF Clone
CAT＃: RC200361
GTF2F2 (Myc-DDK-tagged)-Human general transcription factor IIF, polypeptide 2, 30kDa (GTF2F2)
ORF Plasmid: tGFP
"NM_004128" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BTF4; RAP30; TF2F2; TFIIF |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200361 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGAGCGCGGGGAACTCGACTTGACCGGCGCCAAACAGAACACAGGAGTGTGGCTAGTCAAGGTTC CTAAATATTTGTCACAGCAATGGGCTAAAGCTTCTGGAAGAGGTGAAGTTGGGAAACTGCGGATTGCCAA GACTCAAGGAAGGACTGAGGTGTCATTTACTTTGAATGAGGATCTTGCAAATATTCATGATATTGGTGGA AAACCAGCTTCAGTCAGTGCTCCTAGAGAACATCCATTTGTCTTGCAAAGTGTTGGAGGACAGACATTAA CAGTATTTACTGAGAGCTCATCAGATAAGCTGTCATTGGAAGGAATAGTGGTACAAAGAGCTGAATGCCG ACCAGCTGCCAGTGAAAACTACATGCGATTAAAAAGATTGCAAATAGAAGAGTCTTCCAAACCAGTGAGG CTATCACAACAGCTGGACAAAGTTGTAACAACCAATTACAAACCTGTTGCTAATCATCAATACAATATCG AATATGAAAGGAAAAAGAAAGAAGACGGAAAGCGAGCTCGAGCTGATAAACAACATGTTTTAGACATGCT ATTTTCAGCCTTTGAGAAACATCAATACTATAATCTTAAGGACTTGGTGGACATCACAAAACAACCTGTG GTGTACCTGAAGGAAATCTTAAAAGAAATTGGTGTTCAGAATGTAAAAGGGATCCACAAAAACACATGGG AGCTGAAGCCAGAGTACAGACACTATCAAGGAGAAGAAAAGAGTGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200361 protein sequence
Red=Cloning site Green=Tags(s) MAERGELDLTGAKQNTGVWLVKVPKYLSQQWAKASGRGEVGKLRIAKTQGRTEVSFTLNEDLANIHDIGG KPASVSAPREHPFVLQSVGGQTLTVFTESSSDKLSLEGIVVQRAECRPAASENYMRLKRLQIEESSKPVR LSQQLDKVVTTNYKPVANHQYNIEYERKKKEDGKRARADKQHVLDMLFSAFEKHQYYNLKDLVDITKQPV VYLKEILKEIGVQNVKGIHKNTWELKPEYRHYQGEEKSD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004128 |
| ORF Size | 747 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004128.3 |
| RefSeq Size | 1469 bp |
| RefSeq ORF | 750 bp |
| Locus ID | 2963 |
| UniProt ID | P13984 |
| Protein Families | Transcription Factors |
| Protein Pathways | Basal transcription factors |
| MW | 28.4 kDa |
| Gene Summary | TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. This subunit shows ATP-dependent DNA-helicase activity.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200361L3 | Lenti ORF clone of Human general transcription factor IIF, polypeptide 2, 30kDa (GTF2F2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200361L4 | Lenti ORF clone of Human general transcription factor IIF, polypeptide 2, 30kDa (GTF2F2), mGFP tagged |
CNY 5,890.00 |
|
| RG200361 | GTF2F2 (tGFP-tagged) - Human general transcription factor IIF, polypeptide 2, 30kDa (GTF2F2) |
CNY 4,000.00 |
|
| SC321338 | GTF2F2 (untagged)-Human general transcription factor IIF, polypeptide 2, 30kDa (GTF2F2) |
CNY 2,400.00 |
