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MCM5 (NM_006739) Human Tagged ORF Clone
CAT＃: RC201668
MCM5 (Myc-DDK-tagged)-Human minichromosome maintenance complex component 5 (MCM5)
ORF Plasmid: tGFP
"NM_006739" in other vectors (7)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CDC46; MGORS8; P1-CDC46 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201668 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGGATTCGACGATCCTGGCATTTTCTACAGCGACAGCTTCGGGGGCGACGCCCAGGCCGACGAGG GGCAGGCCCGCAAATCGCAGCTGCAGAGGCGCTTCAAGGAGTTCCTGCGGCAGTACCGAGTGGGCACCGA CCGCACGGGCTTCACCTTCAAATACAGGGATGAACTCAAGCGGCATTACAACCTGGGGGAGTACTGGATT GAGGTGGAGATGGAGGATCTGGCCAGCTTTGATGAGGACCTGGCCGACTACTTGTACAAGCAGCCAGCCG AGCACCTGCAGCTGCTGGAGGAAGCTGCCAAGGAGGTAGCTGATGAGGTGACCCGGCCCCGGCCTTCTGG GGAGGAGGTGCTCCAGGACATCCAGGTCATGCTCAAGTCGGACGCCAGCCCTTCCAGCATTCGTAGCCTG AAGTCGGACATGATGTCACACCTGGTGAAGATCCCTGGCATCATCATCGCGGCCTCTGCGGTCCGTGCCA AGGCCACCCGCATCTCTATCCAGTGCCGCAGCTGCCGCAACACCCTCACCAACATTGCCATGCGCCCTGG CCTCGAGGGCTATGCCCTGCCCAGGAAGTGCAACACAGATCAGGCTGGGCGCCCCAAATGCCCATTGGAC CCGTACTTCATCATGCCCGACAAATGCAAATGCGTGGACTTCCAGACCCTGAAGCTGCAGGAGCTGCCTG ATGCAGTCCCCCACGGGGAGATGCCCAGACACATGCAGCTCTACTGCGACAGGTACCTGTGTGACAAGGT CGTCCCTGGGAACAGGGTTACCATCATGGGCATCTACTCCATCAAGAAGTTTGGCCTGACCACCAGCAGG GGCCGTGACAGGGTGGGCGTGGGCATCCGAAGCTCCTACATCCGTGTCCTGGGCATCCAGGTGGACACAG ATGGCTCTGGCCGCAGCTTTGCTGGGGCCGTGAGCCCCCAGGAGGAGGAGGAGTTCCGTCGCCTGGCTGC CCTCCCAAATGTCTATGAGGTCATCTCCAAGAGCATCGCCCCCTCCATCTTTGGGGGCACAGACATGAAG AAGGCCATTGCCTGCCTGCTCTTTGGGGGCTCCCGAAAGAGGCTCCCTGATGGACTTACTCGCCGAGGAG ACATCAACCTGCTGATGCTAGGGGACCCTGGGACAGCCAAGTCCCAGCTTCTGAAGTTTGTGGAGAAGTG TTCTCCCATTGGGGTATACACGTCTGGGAAAGGCAGCAGCGCAGCTGGACTGACAGCCTCGGTGATGAGG GACCCTTCGTCCCGGAATTTCATCATGGAGGGCGGAGCCATGGTCCTGGCCGATGGTGGGGTCGTCTGTA TTGACGAGTTTGACAAGATGCGAGAAGATGACCGTGTGGCAATCCACGAAGCCATGGAGCAGCAGACCAT CTCTATCGCCAAGGCTGGGATCACCACCACCCTGAACTCCCGCTGCTCCGTCCTGGCTGCTGCCAACTCA GTGTTCGGCCGCTGGGATGAGACGAAGGGGGAGGACAACATTGACTTCATGCCCACCATCTTGTCGCGCT TCGACATGATCTTCATCGTCAAGGATGAGCACAATGAGGAGAGGGATGTGATGCTGGCCAAGCATGTCAT CACTCTGCACGTGAGCGCACTGACACAGACACAGGCTGTGGAGGGCGAGATTGACCTGGCCAAGCTGAAG AAGTTTATTGCCTACTGCCGAGTGAAATGTGGCCCCCGGCTGTCAGCAGAGGCTGCAGAGAAACTGAAGA ACCGCTACATCATCATGCGGAGCGGGGCCCGTCAGCACGAGAGGGACAGTGACCGCCGCTCCAGCATCCC CATCACTGTGCGGCAGCTGGAGGCCATTGTGCGCATCGCGGAAGCCCTCAGCAAGATGAAGCTGCAGCCC TTCGCCACAGAGGCAGATGTGGAGGAGGCCCTGCGGCTCTTCCAAGTGTCCACGTTGGATGCTGCCTTGT CCGGTACCCTGTCAGGGGTGGAGGGCTTCACCAGCCAGGAGGACCAGGAGATGCTGAGCCGCATCGAGAA GCAGCTCAAGCGCCGCTTTGCCATTGGCTCCCAGGTGTCTGAGCACAGCATCATCAAGGACTTCACCAAG CAGAAATACCCGGAGCACGCCATCCACAAGGTGCTGCAGCTCATGCTGCGGCGCGGCGAGATCCAGCATC GCATGCAGCGCAAGGTTCTCTACCGCCTCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201668 protein sequence
Red=Cloning site Green=Tags(s) MSGFDDPGIFYSDSFGGDAQADEGQARKSQLQRRFKEFLRQYRVGTDRTGFTFKYRDELKRHYNLGEYWI EVEMEDLASFDEDLADYLYKQPAEHLQLLEEAAKEVADEVTRPRPSGEEVLQDIQVMLKSDASPSSIRSL KSDMMSHLVKIPGIIIAASAVRAKATRISIQCRSCRNTLTNIAMRPGLEGYALPRKCNTDQAGRPKCPLD PYFIMPDKCKCVDFQTLKLQELPDAVPHGEMPRHMQLYCDRYLCDKVVPGNRVTIMGIYSIKKFGLTTSR GRDRVGVGIRSSYIRVLGIQVDTDGSGRSFAGAVSPQEEEEFRRLAALPNVYEVISKSIAPSIFGGTDMK KAIACLLFGGSRKRLPDGLTRRGDINLLMLGDPGTAKSQLLKFVEKCSPIGVYTSGKGSSAAGLTASVMR DPSSRNFIMEGGAMVLADGGVVCIDEFDKMREDDRVAIHEAMEQQTISIAKAGITTTLNSRCSVLAAANS VFGRWDETKGEDNIDFMPTILSRFDMIFIVKDEHNEERDVMLAKHVITLHVSALTQTQAVEGEIDLAKLK KFIAYCRVKCGPRLSAEAAEKLKNRYIIMRSGARQHERDSDRRSSIPITVRQLEAIVRIAEALSKMKLQP FATEADVEEALRLFQVSTLDAALSGTLSGVEGFTSQEDQEMLSRIEKQLKRRFAIGSQVSEHSIIKDFTK QKYPEHAIHKVLQLMLRRGEIQHRMQRKVLYRLK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006739 |
| ORF Size | 2202 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006739.4 |
| RefSeq Size | 2568 bp |
| RefSeq ORF | 2205 bp |
| Locus ID | 4174 |
| UniProt ID | P33992 |
| Domains | MCM |
| Protein Families | Stem cell - Pluripotency, Transcription Factors |
| Protein Pathways | Cell cycle, DNA replication |
| MW | 82.3 kDa |
| Gene Summary | The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201668L1 | Lenti ORF clone of Human minichromosome maintenance complex component 5 (MCM5), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201668L2 | Lenti ORF clone of Human minichromosome maintenance complex component 5 (MCM5), mGFP tagged |
CNY 5,890.00 |
|
| RC201668L3 | Lenti ORF clone of Human minichromosome maintenance complex component 5 (MCM5), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201668L4 | Lenti ORF clone of Human minichromosome maintenance complex component 5 (MCM5), mGFP tagged |
CNY 5,890.00 |
|
| RG201668 | MCM5 (tGFP-tagged) - Human minichromosome maintenance complex component 5 (MCM5) |
CNY 4,370.00 |
|
| SC115899 | MCM5 (untagged)-Human minichromosome maintenance complex component 5 (MCM5) |
CNY 2,950.00 |
|
| SC323774 | MCM5 (untagged)-Human minichromosome maintenance complex component 5 (MCM5) |
CNY 2,950.00 |
