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USP13 (NM_003940) Human Tagged ORF Clone
CAT＃: RC202190
USP13 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 13 (isopeptidase T-3) (USP13)
ORF Plasmid: tGFP
"NM_003940" in other vectors (6)
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CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | IsoT-3; ISOT3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202190 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCGCCGGGGCGCCCTGTTCGGCATGCCGGGCGGCAGCGGAGGCAGGAAGATGGCTGCAGGAGACA TCGGCGAGCTGCTAGTGCCCCACATGCCCACGATCCGCGTGCCCAGGTCCGGCGACAGGGTCTACAAGAA CGAGTGCGCCTTCTCCTACGACTCTCCCAATTCTGAAGGTGGACTCTATGTATGCATGAATACATTTTTG GCCTTTGGAAGGGAACATGTTGAAAGACATTTTCGAAAAACTGGACAGAGTGTATACATGCACCTGAAAA GACATGTGCGAGAGAAGGTAAGAGGGGCGTCTGGTGGAGCGTTACCAAAAAGGAGGAATTCCAAGATTTT TTTAGATCTAGATACTGATGACGATTTAAATAGCGACGATTATGAATATGAAGATGAAGCCAAACTTGTT ATATTCCCAGATCACTATGAAATAGCACTACCAAATATTGAGGAGTTACCAGCCCTGGTAACAATTGCTT GTGATGCAGTTCTCAGCTCAAAATCTCCATACAGAAAGCAGGACCCAGACACGTGGGAAAATGAATTGCC AGTATCTAAATATGCCAACAACCTCACCCAGCTGGACAATGGAGTCAGGATTCCTCCAAGTGGTTGGAAG TGTGCCAGATGCGACCTGCGAGAAAACCTCTGGTTGAATCTGACTGACGGCTCTGTCCTGTGTGGAAAGT GGTTCTTTGACAGCTCTGGGGGCAACGGGCATGCGCTGGAGCATTACAGAGACATGGGCTACCCACTAGC CGTGAAACTGGGAACCATCACTCCTGACGGGGCAGATGTTTATTCTTTTCAAGAAGAAGAACCTGTTTTG GATCCTCATTTGGCCAAGCACTTAGCGCATTTTGGAATTGATATGCTTCATATGCATGGGACAGAGAATG GGCTCCAGGACAATGACATCAAGCTGAGGGTCAGTGAGTGGGAAGTGATCCAGGAGTCGGGCACGAAACT GAAGCCAATGTATGGTCCTGGCTACACGGGTCTGAAGAACCTGGGCAACAGCTGCTATCTCAGCTCTGTC ATGCAGGCCATCTTCAGCATCCCAGAATTCCAGAGAGCGTATGTAGGAAACCTTCCCAGAATATTTGACT ACTCGCCTTTAGATCCAACACAAGATTTCAACACACAGATGACTAAGTTAGGACATGGCCTTCTCTCAGG CCAGTATTCAAAGCCTCCGGTGAAATCTGAACTCATTGAACAGGTGATGAAGGAGGAGCACAAGCCACAG CAGAACGGGATCTCTCCGCGCATGTTTAAGGCCTTTGTAAGCAAGAGCCACCCGGAATTCTCCTCTAACA GGCAGCAAGATGCCCAGGAATTCTTCTTGCACCTGGTGAATCTAGTAGAGAGGAACCGCATCGGCTCAGA AAACCCAAGCGATGTTTTTCGTTTTTTGGTGGAAGAACGCATTCAGTGCTGTCAGACCCGGAAAGTCCGC TACACGGAGAGGGTGGATTACCTGATGCAGTTACCTGTGGCCATGGAGGCGGCAACCAACAAGGATGAAC TGATCGCTTATGAACTAACGAGAAGGGAAGCAGAAGCAAACAGAAGACCCCTTCCTGAGTTGGTACGTGC CAAGATACCATTTAGTGCCTGCCTTCAGGCCTTCTCTGAACCAGAAAATGTTGATGATTTCTGGAGCAGT GCCCTACAAGCAAAGTCTGCGGGTGTGAAAACATCTCGCTTTGCTTCATTCCCTGAATACTTGGTAGTGC AGATAAAGAAGTTCACTTTTGGTCTTGACTGGGTTCCCAAAAAATTTGATGTTTCTATTGATATGCCAGA CCTACTTGATATCAACCATCTCCGAGCCAGGGGGTTACAGCCAGGAGAGGAAGAACTTCCAGACATCAGC CCCCCCATAGTCATTCCTGATGACTCAAAAGATCGCCTGATGAACCAATTGATAGACCCATCAGACATCG ATGAGTCATCAGTGATGCAGCTGGCCGAGATGGGTTTCCCGCTGGAAGCATGTCGCAAGGCTGTGTACTT CACTGGAAATATGGGCGCCGAGGTGGCCTTCAACTGGATCATTGTTCACATGGAAGAGCCAGATTTTGCT GAGCCGCTGACCATGCCTGGTTATGGAGGGGCAGCTTCTGCTGGAGCCTCTGTTTTTGGTGCTTCTGGAC TGGATAACCAACCTCCAGAGGAAATCGTAGCTATCATCACCTCCATGGGATTTCAGCGAAATCAGGCTAT TCAGGCACTACGAGCAACGAATAATAACCTGGAAAGAGCACTGGATTGGATCTTTAGCCACCCTGAGTTT GAAGAAGACAGTGATTTTGTGATTGAGATGGAGAATAATGCCAATGCAAACATTATTTCTGAGGCCAAGC CCGAAGGACCTAGAGTCAAGGATGGATCTGGAACATATGAGCTATTTGCATTCATCAGTCACATGGGAAC ATCCACAATGAGTGGTCATTACATTTGCCATATCAAAAAGGAAGGAAGATGGGTGATTTACAATGACCAC AAAGTTTGTGCCTCAGAAAGGCCCCCTAAAGACCTGGGCTACATGTACTTTTACCGCAGGATACCAAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202190 protein sequence
Red=Cloning site Green=Tags(s) MQRRGALFGMPGGSGGRKMAAGDIGELLVPHMPTIRVPRSGDRVYKNECAFSYDSPNSEGGLYVCMNTFL AFGREHVERHFRKTGQSVYMHLKRHVREKVRGASGGALPKRRNSKIFLDLDTDDDLNSDDYEYEDEAKLV IFPDHYEIALPNIEELPALVTIACDAVLSSKSPYRKQDPDTWENELPVSKYANNLTQLDNGVRIPPSGWK CARCDLRENLWLNLTDGSVLCGKWFFDSSGGNGHALEHYRDMGYPLAVKLGTITPDGADVYSFQEEEPVL DPHLAKHLAHFGIDMLHMHGTENGLQDNDIKLRVSEWEVIQESGTKLKPMYGPGYTGLKNLGNSCYLSSV MQAIFSIPEFQRAYVGNLPRIFDYSPLDPTQDFNTQMTKLGHGLLSGQYSKPPVKSELIEQVMKEEHKPQ QNGISPRMFKAFVSKSHPEFSSNRQQDAQEFFLHLVNLVERNRIGSENPSDVFRFLVEERIQCCQTRKVR YTERVDYLMQLPVAMEAATNKDELIAYELTRREAEANRRPLPELVRAKIPFSACLQAFSEPENVDDFWSS ALQAKSAGVKTSRFASFPEYLVVQIKKFTFGLDWVPKKFDVSIDMPDLLDINHLRARGLQPGEEELPDIS PPIVIPDDSKDRLMNQLIDPSDIDESSVMQLAEMGFPLEACRKAVYFTGNMGAEVAFNWIIVHMEEPDFA EPLTMPGYGGAASAGASVFGASGLDNQPPEEIVAIITSMGFQRNQAIQALRATNNNLERALDWIFSHPEF EEDSDFVIEMENNANANIISEAKPEGPRVKDGSGTYELFAFISHMGTSTMSGHYICHIKKEGRWVIYNDH KVCASERPPKDLGYMYFYRRIPS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003940 |
| ORF Size | 2589 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003940.3 |
| RefSeq Size | 7933 bp |
| RefSeq ORF | 2592 bp |
| Locus ID | 8975 |
| UniProt ID | Q92995 |
| Domains | UBA, UCH, zf-UBP |
| Protein Families | Druggable Genome, Protease |
| MW | 97.3 kDa |
| Gene Summary | Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum-associated degradation (ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34-containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Also regulates ERAD through the deubiquitination of UBL4A a component of the BAG6/BAT3 complex. Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro; however, additional experiments are required to confirm such data.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
USP13 Enzyme Regulates Siah2 Ligase Stability and Activity via Noncatalytic Ubiquitin-binding Domains
,Marzia Scortegagna, Tony Subtil, Jianfei Qi, Hyungsoo Kim, Wenhui Zhao, Wei Gu, Harriet Kluger, and Ze'ev A. Ronai,
J. Biol. Chem., Aug 2011; 286: 27333 - 27341.
[USP13]
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Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202190L1 | Lenti ORF clone of Human ubiquitin specific peptidase 13 (isopeptidase T-3) (USP13), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202190L2 | Lenti ORF clone of Human ubiquitin specific peptidase 13 (isopeptidase T-3) (USP13), mGFP tagged |
CNY 5,890.00 |
|
| RC202190L3 | Lenti ORF clone of Human ubiquitin specific peptidase 13 (isopeptidase T-3) (USP13), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202190L4 | Lenti ORF clone of Human ubiquitin specific peptidase 13 (isopeptidase T-3) (USP13), mGFP tagged |
CNY 5,890.00 |
|
| RG202190 | USP13 (tGFP-tagged) - Human ubiquitin specific peptidase 13 (isopeptidase T-3) (USP13) |
CNY 4,370.00 |
|
| SC117705 | USP13 (untagged)-Human ubiquitin specific peptidase 13 (isopeptidase T-3) (USP13) |
CNY 2,950.00 |
