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HCE (RNGTT) (NM_003800) Human Tagged ORF Clone
CAT＃: RC202229
RNGTT (Myc-DDK-tagged)-Human RNA guanylyltransferase and 5'-phosphatase (RNGTT)
ORF Plasmid: tGFP
"NM_003800" in other vectors (6)
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CNY 3,990.00
Product images
CNY 300.00
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CAP1A; hCAP; HCE; HCE1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202229 representing NM_003800
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCACAACAAGATCCCGCCGCGGTGGCTGAACTGTCCCCGGCGCGGCCAGCCGGTGGCAGGAAGAT TCTTACCTCTGAAGACAATGTTAGGACCAAGATATGATAGTCAAGTTGCTGAAGAAAATCGGTTCCATCC CAGCATGCTCTCAAATTACCTAAAGAGCCTAAAGGTTAAAATGGGCTTGTTGGTGGACCTGACAAATACT TCAAGGTTCTATGACCGAAATGACATAGAAAAAGAAGGAATCAAATATATAAAACTTCAGTGTAAAGGAC ATGGTGAGTGCCCTACCACTGAGAATACTGAGACCTTTATTCGTCTGTGTGAGCGGTTTAATGAAAGAAA TCCACCTGAACTTATAGGTGTTCATTGTACTCATGGCTTCAATCGCACTGGTTTCCTCATATGTGCCTTT TTGGTGGAGAAAATGGATTGGAGTATCGAAGCAGCAGTTGCTACTTTTGCCCAAGCCAGACCACCAGGAA TCTACAAGGGTGATTATTTGAAGGAACTTTTTCGTCGGTATGGTGACATAGAGGAAGCACCACCCCCACC TCTATTGCCAGATTGGTGTTTTGAGGATGATGAAGACGAAGATGAGGATGAGGATGGAAAGAAGGAATCA GAACCCGGGTCAAGTGCTTCTTTTGGCAAAAGGAGAAAAGAACGGTTAAAACTGGGCGCTATTTTCTTGG AAGGTGTTACTGTTAAAGGTGTAACTCAAGTAACAACACAACCAAAGTTAGGAGAGGTACAGCAGAAGTG TCATCAATTCTGTGGCTGGGAAGGGTCTGGATTCCCTGGAGCACAGCCTGTTTCCATGGACAAGCAAAAT ATTAAACTTTTAGACCTGAAGCCATACAAAGTAAGCTGGAAAGCAGATGGTACTCGGTACATGATGTTGA TTGATGGCACAAATGAAGTTTTTATGATTGATAGAGACAATTCAGTATTTCATGTTTCAAATCTGGAATT TCCATTTCGTAAAGATCTTCGTATGCATTTATCAAATACTCTCTTGGATGGCGAGATGATTATTGACAGA GTAAATGGACAGGCTGTTCCTAGATATTTGATATATGACATAATTAAATTCAATTCACAGCCCGTTGGAG ATTGTGATTTTAATGTTCGTCTGCAGTGTATAGAACGAGAAATTATAAGTCCTCGACACGAAAAAATGAA GACTGGGCTCATTGACAAAACACAGGAACCATTTAGCGTCAGAAATAAGCCGTTTTTTGACATCTGTACT TCAAGAAAGCTACTTGAAGGAAATTTTGCCAAAGAAGTGAGCCATGAAATGGATGGACTTATTTTTCAGC CTACTGGAAAATACAAACCTGGTCGATGTGATGATATTTTGAAATGGAAGCCTCCCAGTCTGAATTCTGT GGATTTTCGTCTAAAAATAACAAGAATGGGAGGAGAAGGGTTACTTCCTCAGAATGTTGGCCTCCTGTAT GTTGGAGGTTATGAAAGACCCTTTGCACAAATCAAGGTGACAAAAGAGCTGAAACAGTATGACAACAAAA TTATAGAATGCAAATTTGAGAACAACAGCTGGGTCTTCATGAGACAGAGAACAGACAAAAGTTTTCCTAA TGCCTACAACACTGCCATGGCTGTGTGTAACAGCATCTCAAACCCTGTCACCAAGGAGATGCTGTTTGAG TTCATCGACAGATGTACTGCAGCTTCTCAAGGACAGAAGCGAAAACATCATCTGGACCCTGACACGGAGC TCATGCCACCACCACCTCCCAAAAGACCACACCCTTTAACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202229 representing NM_003800
Red=Cloning site Green=Tags(s) MAHNKIPPRWLNCPRRGQPVAGRFLPLKTMLGPRYDSQVAEENRFHPSMLSNYLKSLKVKMGLLVDLTNT SRFYDRNDIEKEGIKYIKLQCKGHGECPTTENTETFIRLCERFNERNPPELIGVHCTHGFNRTGFLICAF LVEKMDWSIEAAVATFAQARPPGIYKGDYLKELFRRYGDIEEAPPPPLLPDWCFEDDEDEDEDEDGKKES EPGSSASFGKRRKERLKLGAIFLEGVTVKGVTQVTTQPKLGEVQQKCHQFCGWEGSGFPGAQPVSMDKQN IKLLDLKPYKVSWKADGTRYMMLIDGTNEVFMIDRDNSVFHVSNLEFPFRKDLRMHLSNTLLDGEMIIDR VNGQAVPRYLIYDIIKFNSQPVGDCDFNVRLQCIEREIISPRHEKMKTGLIDKTQEPFSVRNKPFFDICT SRKLLEGNFAKEVSHEMDGLIFQPTGKYKPGRCDDILKWKPPSLNSVDFRLKITRMGGEGLLPQNVGLLY VGGYERPFAQIKVTKELKQYDNKIIECKFENNSWVFMRQRTDKSFPNAYNTAMAVCNSISNPVTKEMLFE FIDRCTAASQGQKRKHHLDPDTELMPPPPPKRPHPLT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003800 |
| ORF Size | 1791 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003800.5 |
| RefSeq Size | 4460 bp |
| RefSeq ORF | 1794 bp |
| Locus ID | 8732 |
| UniProt ID | O60942 |
| Domains | mRNA_cap_C |
| Protein Families | Druggable Genome, Phosphatase |
| MW | 68.4 kDa |
| Gene Summary | Bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202229L1 | Lenti ORF clone of Human RNA guanylyltransferase and 5'-phosphatase (RNGTT), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202229L2 | Lenti ORF clone of Human RNA guanylyltransferase and 5'-phosphatase (RNGTT), mGFP tagged |
CNY 5,890.00 |
|
| RC202229L3 | Lenti ORF clone of Human RNA guanylyltransferase and 5'-phosphatase (RNGTT), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202229L4 | Lenti ORF clone of Human RNA guanylyltransferase and 5'-phosphatase (RNGTT), mGFP tagged |
CNY 5,890.00 |
|
| RG202229 | RNGTT (tGFP-tagged) - Human RNA guanylyltransferase and 5'-phosphatase (RNGTT) |
CNY 4,370.00 |
|
| SC117771 | RNGTT (untagged)-Human RNA guanylyltransferase and 5'-phosphatase (RNGTT) |
CNY 2,950.00 |
