DUSP12 (NM_007240) Human Tagged ORF Clone
CAT#: RC202411
DUSP12 (Myc-DDK-tagged)-Human dual specificity phosphatase 12 (DUSP12)
ORF Plasmid: tGFP
"NM_007240" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | DUSP1; YVH1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC202411 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGGAGGCTCCGGGCCCGAGTGATGGCTGCGAGCTCAGCAACCCCAGCGCCAGCAGAGTCAGCTGTG CCGGGCAGATGCTGGAAGTGCAGCCAGGATTGTATTTCGGTGGGGCCGCGGCCGTCGCGGAGCCAGATCA CCTGAGGGAAGCGGGCATCACGGCCGTGCTAACAGTGGACTCGGAGGAGCCCAGCTTCAAGGCGGGGCCT GGGGTCGAGGATCTATGGCGCCTCTTCGTGCCAGCGCTGGACAAACCCGAGACGGACCTACTCAGCCATC TGGACCGGTGCGTGGCCTTCATCGGTCAGGCCCGCGCTGAGGGCCGTGCGGTGTTGGTGCACTGTCATGC AGGAGTCAGTCGAAGTGTGGCCATAATAACTGCTTTTCTCATGAAGACTGACCAACTTCCCTTTGAAAAA GCCTATGAAAAGCTCCAGATTCTCAAACCAGAGGCTAAGATGAATGAGGGGTTTGAGTGGCAACTGAAAT TATACCAGGCAATGGGATATGAAGTGGATACCTCTAGTGCAATTTATAAGCAATATCGTTTACAAAAGGT TACAGAGAAGTATCCAGAATTGCAGAATTTACCTCAAGAACTCTTTGCTGTTGACCCAACTACCGTTTCA CAAGGATTGAAAGATGAGGTTCTCTACAAGTGTAGAAAGTGCAGGCGATCATTATTTCGAAGTTCTAGTA TTCTGGATCACCGTGAAGGAAGTGGACCTATAGCCTTTGCCCACAAGAGAATGACACCATCTTCCATGCT TACCACAGGGAGGCAAGCTCAATGTACATCTTATTTCATTGAACCTGTACAGTGGATGGAATCTGCTTTG TTGGGAGTGATGGATGGACAGCTTCTTTGCCCAAAATGCAGTGCCAAGTTGGGTTCCTTCAACTGGTATG GTGAACAGTGCTCTTGTGGTAGGTGGATAACACCTGCTTTTCAAATACATAAGAATAGAGTGGATGAAAT GAAAATATTGCCTGTTTTGGGATCACAAACAGGAAAAATA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202411 protein sequence
Red=Cloning site Green=Tags(s) MLEAPGPSDGCELSNPSASRVSCAGQMLEVQPGLYFGGAAAVAEPDHLREAGITAVLTVDSEEPSFKAGP GVEDLWRLFVPALDKPETDLLSHLDRCVAFIGQARAEGRAVLVHCHAGVSRSVAIITAFLMKTDQLPFEK AYEKLQILKPEAKMNEGFEWQLKLYQAMGYEVDTSSAIYKQYRLQKVTEKYPELQNLPQELFAVDPTTVS QGLKDEVLYKCRKCRRSLFRSSSILDHREGSGPIAFAHKRMTPSSMLTTGRQAQCTSYFIEPVQWMESAL LGVMDGQLLCPKCSAKLGSFNWYGEQCSCGRWITPAFQIHKNRVDEMKILPVLGSQTGKI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_007240 |
ORF Size | 1020 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007240.1, NP_009171.1 |
RefSeq Size | 1271 bp |
RefSeq ORF | 1023 bp |
Locus ID | 11266 |
UniProt ID | Q9UNI6 |
Domains | DSPc |
Protein Families | Druggable Genome, Phosphatase |
MW | 37.7 kDa |
Gene Summary | The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product is the human ortholog of the Saccharomyces cerevisiae YVH1 protein tyrosine phosphatase. It is localized predominantly in the nucleus, and is novel in that it contains, and is regulated by a zinc finger domain. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC202411L1 | Lenti ORF clone of Human dual specificity phosphatase 12 (DUSP12), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC202411L2 | Lenti ORF clone of Human dual specificity phosphatase 12 (DUSP12), mGFP tagged |
CNY 5,890.00 |
|
RC202411L3 | Lenti ORF clone of Human dual specificity phosphatase 12 (DUSP12), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC202411L4 | Lenti ORF clone of Human dual specificity phosphatase 12 (DUSP12), mGFP tagged |
CNY 5,890.00 |
|
RG202411 | DUSP12 (tGFP-tagged) - Human dual specificity phosphatase 12 (DUSP12) |
CNY 4,370.00 |
|
SC319165 | DUSP12 (untagged)-Human dual specificity phosphatase 12 (DUSP12) |
CNY 2,950.00 |