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RPRM (NM_019845) Human Tagged ORF Clone
CAT＃: RC202634
RPRM (Myc-DDK-tagged)-Human reprimo, TP53 dependent G2 arrest mediator candidate (RPRM)
ORF Plasmid: tGFP
"NM_019845" in other vectors (6)
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CNY 1,200.00
CNY 3,705.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | REPRIMO |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202634 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATCCGGCCCTAGGCAACCAGACGGACGTGGCGGGCCTGTTCCTGGCCAACAGCAGCGAGGCGCTGG AGCGAGCCGTGCGCTGCTGCACCCAGGCGTCCGTGGTGACCGACGACGGCTTCGCGGAGGGAGGCCCGGA CGAGCGTAGCCTGTACATAATGCGCGTGGTGCAGATCGCGGTCATGTGCGTGCTCTCACTCACCGTGGTC TTCGGCATCTTCTTCCTCGGCTGCAATCTGCTCATCAAGTCCGAGGGCATGATCAACTTCCTCGTGAAGG ACCGGAGGCCGTCTAAGGAGGTGGAGGCGGTGGTCGTGGGGCCCTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202634 protein sequence
Red=Cloning site Green=Tags(s) MNPALGNQTDVAGLFLANSSEALERAVRCCTQASVVTDDGFAEGGPDERSLYIMRVVQIAVMCVLSLTVV FGIFFLGCNLLIKSEGMINFLVKDRRPSKEVEAVVVGPY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019845 |
| ORF Size | 327 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019845.3 |
| RefSeq Size | 1496 bp |
| RefSeq ORF | 330 bp |
| Locus ID | 56475 |
| UniProt ID | Q9NS64 |
| Protein Families | Transmembrane |
| Protein Pathways | p53 signaling pathway |
| MW | 11.8 kDa |
| Gene Summary | May be involved in the regulation of p53-dependent G2 arrest of the cell cycle. Seems to induce cell cycle arrest by inhibiting CDK1 activity and nuclear translocation of the CDC2 cyclin B1 complex (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Loss of Expression of Reprimo, a p53-induced Cell Cycle Arrest Gene, Correlates with Invasive Stage of Tumor Progression and p73 Expression in Gastric Cancer
,Saavedra, K;Valbuena, J;Olivares, W;Marchant, MJ;Rodríguez, A;Torres-Estay, V;Carrasco-Avino, G;Guzmán, L;Aguayo, F;Roa, JC;Corvalán, AH;,
PLoS ONE
,PubMed ID 25954972
[RPRM]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202634L1 | Lenti ORF clone of Human reprimo, TP53 dependent G2 arrest mediator candidate (RPRM), Myc-DDK-tagged |
CNY 3,600.00 |
|
| RC202634L2 | Lenti ORF clone of Human reprimo, TP53 dependent G2 arrest mediator candidate (RPRM), mGFP tagged |
CNY 5,890.00 |
|
| RC202634L3 | Lenti ORF clone of Human reprimo, TP53 dependent G2 arrest mediator candidate (RPRM), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202634L4 | Lenti ORF clone of Human reprimo, TP53 dependent G2 arrest mediator candidate (RPRM), mGFP tagged |
CNY 5,890.00 |
|
| RG202634 | RPRM (tGFP-tagged) - Human reprimo, TP53 dependent G2 arrest mediator candidate (RPRM) |
CNY 2,800.00 |
|
| SC108659 | RPRM (untagged)-Human reprimo, TP53 dependent G2 arrest mediator candidate (RPRM) |
CNY 1,200.00 |
